Efeito da fonte de carbono e nitrogênio na produção de β 1,3 glucanases por Trichoderma asperellum
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2008-05-29
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Universidade Federal de Goiás
Resumo
The β-1.3-glucanases have several functions in the cell as fungal metabolic in
hydrolysis of polysaccharides for possible cellular assimilation; morphogenetic
function in the hydrolysis or modifications of the cell wall, which includes growth and
extension of the wall, altering the structure and composition of the wall and autolysis.
In addition to presenting ecological importance, since these enzymes are involved in
biological control, through the process of mycoparasitism. The gender Trichoderma
comprises a group of filamentous fungi, saprophytic soil, found on decomposing
organic matter in the rhizosphere, and some plants. Considering the importance of
β-1.3 glucanases proposing in this study to evaluate the production of
β-1.3 - glucanases by T. asperellum using different sources of carbon and nitrogen.
The enzyme was produced using cell wall of Rizoctonia solani (PCRS), chitin,
chitosan, starch, cellulose, sucrose, maltose, lactose, celobiose and glucose. The
best production of enzymes was obtained in the middle containing PCRS, with two
bands of activity of polyacrylamide gel were found. Furthermore, the effect of varying
concentrations of ammonium sulfate (2, 4, 6, 8, 20, 40 and 60 mM) in the production
of β-1.3-glucanase was evaluated during the growth of the fungus in PCRS. A great
increase in activity of the enzyme was detected in the presence of nitrogen source.
We can see that there was a considerable increase in the expression of the enzyme
of low molecular weight at this culture conditions.
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SILVA, Regiane Christine da. Effect of source of carbon and nitrogen in the production of β-1 ,3-glucanase by Trichoderma asperellum. 2008. 48 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2008.