Estudo da formação de biofilmes bacterianos em endopróteses (“stent”) e inibição da formação de biofilme por peptídeos antimicrobianos de venenos de artrópodes
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2019-11-04
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Universidade Federal de Goiás
Resumo
The use of synthetic materials as temporary or permanent insertion in the
body can result in infections associated with the colonization of these
materials. The colonization of these materials can result in bacterial biofilms
formation. Acinetobacter baumannii infections are difficult to treat due to
biofilm formation and resistance to multiple drugs. Staphylococcus strains
are able to form biofilm and to resist against antibiotics, in addition of being
associated with endocarditis. Thus, in this thesis we hypothesize that hospital
acquired bacteria were able to form biofilm in vascular stent. In this study,
the biofilm formation in polystyrene plates and on coronary stents of three
isolates of multidrug-resistant A. baumannii (MDR) AB 02, AB 53 and AB 72
were evaluated. The biofilm formation in coronary stents were analyzed by
scanning electron microscopy. The antimicrobial peptides from wasps
(Agelaia-MPI, Polydia-MPII, Polydim-I) and scorpion (Con10 and NDBP 5.8)
were used to determine the minimum inhibitory concentration (MIC) and
biofilm eradication (MBEC) of A. baumannii on polystyrene plates.
Additionally, methicillin resistant Staphylococcus spp was also used to
evaluate the ability of peptides to inhibit biofilm formation. A. baumannii
MDR in contact with the vascular stent adhered to the biomaterial and
initiated the formation of bacterial biofilm. The MIC of the wasp peptides
against strains of A. baumannii MDR ranged from 3.12 to 6.25 μM and those
of scorpion from 6.25 to 25 μM. The MBEC of Agelaia-MPI and Polybia-MPII
was 6.25 μM. Con10 presented a 6.25 μM MBEC for the AB 72 strain and
12.5 μM for the AB 02 and AB 53 strains and the NBDP 5.8 inhibited at the
25 μM concentration. Agelaia-MPI inhibited the biofilm dispersion of AB 02
and AB 53 at 6.25 μM concentration and of AB 72 at 12.5 μM. Polybia-MPII
inhibited at the concentration 6.25 μM. Agelaia-MPI and Polybia-MPII
inhibited mature biofilms at 6.25 μM. Because Agelaia and Polybia peptides
presented the best inhibitory performance they were tested against
Staphylococcus ssp. Polybia-MPII and Agelaia-MPI showed MIC and MBEC of
12.5 μM. We conclude that A. baumannii forms biofilm in both plates and on
vascular stents. The AMPs from wasp venoms (Agelaia-MPI and Polydia-MPII)
prevent the biofilm formation of both A. baumannii and Staphylococcus
epidermidis. These peptides were also able to reduce the bacterial load of
biofilm-containing biomaterials. Therefore, we suggest that the Agelaia-MPI
and Polybia-MPII antimicrobial peptides may be modified to coat biomaterials
and prevent biofilm formation as well as for the treatment of individuals
afflicted with contaminated biomaterials.
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NEVES, R. C. Estudo da formação de biofilmes bacterianos em endopróteses (“stent”) e inibição da formação de biofilme por peptídeos antimicrobianos de venenos de artrópodes. 2019. 78 f. Tese (Doutorado em Biotecnologia e Biodiversidade em Rede Pró-Centro-Oeste) - Universidade Federal de Goiás, Goiânia, 2019.