Avaliação da atividade genotóxica e antigenotóxica de Celtis iguanaea (Jacq.) Sargent em bactérias e camundongos
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2010-02-24
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Universidade Federal de Goiás
Resumo
Celtis iguanaea (Jacq.) Sargent is a member of the Cannabaceae family and
popularly known as esporão de galo. Ethnobotanical surveys of cerrado native
plants show that the tea leaves of C. iguanaea is used in traditional medicine for
body aches, asthma, cramps, poor digestion, urinary infections, kidney
disfunctions, as a stimulant or as a diuretic.The purpose of the present work
was to evaluate the possible cytotoxic, genotoxic and antigenotoxic effects of
the leaves aqueous extract of C. iguanaea by the prophage λ induction test
(SOS Inductest) and the micronucleous test in mice bone marrow. The SOS
Inductest was performed according to Moreau (1976), using lysogenic WP2s(λ)
and indicator RJF013 strains derived from Escherichia coli. WP2s(λ) cultures
were treated with different doses of the extract (0.5, 1, 2.5, and 5 mg)
(evaluation of genotoxicity) or co-treated with a single dose of MMC (0,5 mg)
and different doses of extract (assessment of antigenotoxicity). Then, the
treated culture was added to the indicator strain (RJF013) and both were
poured on plates in LB(1/2) medium. For the evaluation of cytotoxicity, cultures of
WP2s(λ) treated with different doses of extract were diluted in M9 buffer and
plated on LB. The micronucleus test was carried out according to Schmid
(1975). To assess the genotoxic activity, animals were treated with Celtis
iguanaea extract (100, 300 and 500 mg/kg concentrations). To evaluate the
antigenotoxic activity, the same doses of extract were treated simultaneously
with 4mg/Kg of MMC. The frequencies of micronucleated polychromatic
erythrocytes (MNPCE) were evaluated at 24h and 48h exposure period except
the negative control (24h). The cytotoxicity was assessed by the polychromatic
and normochromatic erythrocytes ratio (PCE/NCE). In both tests the obtained
results demonstrated anticytotoxic activity and absence of genotoxicity and
cytotoxicity of the Celtis iguanaea leaves extract. The extract also showed
partial antigenotoxic activity in bacteria and antigenotoxic activity in mice.
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BORGES, Flávio Fernandes Veloso. Avaliação da atividade genotóxica e antigenotóxica de Celtis iguanaea (Jacq.) Sargent em bactérias e camundongos. 2010. 133 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Goiás, Goiânia, 2010.