Cultivo de embriões bovinos em estágio de blastocisto em meio sequencial, após maturação e fertilização de oócitos in vitro, de novilhas e vacas velhas: comparação entre métodos de congelamento lento, computadorizado e ultra-rápido
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2004-03-01
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Universidade Federal de Goiás
Resumo
Studied in this work maturation in vitro oocytes collected from ovaries obtained from a slaughterhouse, without any hormonal stimulation and, comparing these data at different stages of maturation between heifers and old cows. The culture medium used for maturation was the In Vitro Fertilization (Scandinavian IVF Science AB. Sweden), where similar results were observed for the two age, presenting a significant correlation (r = 0.9991 and p <0.0001) for stages of maturation, despite the maturing rate for new cows have better 50.5%, and for the old cows of 31.3%, there was no statistical difference. The same is repeated for the fertilization of young cows to 98.1% and 86% for old cows. For the blastocyst formation rate, 17.14% to 22.58% and old cows to young cows. We tested two types of cryopreservation for embryos in the blastocyst stage, the ultra-fast freezing, using dimethyl sulfoxide-cryoprotector (DMSO), and the computerized slow freezing with cryo chambers - Australian, using glycerol as cryoprotector. Embryos at the new cows DMSO showed a 14% survival rate and 73% glycerol, and the old cows were 31% DMSO and 63% glycerol. The rehydration rate of re-expansion of blastocysts after thawing was 15% with 15% DMSO and 38% glycerol for old cows and 14% DMSO and 55% glycerol for new cows. Spontaneous Hatching was observed only for the embryos thawed freezing with glycerol for young cows. In the statistical calculation was no significance between the results of survival, to total frozen and thawed embryos, between the slow freezing methods and the ultra-fast slow cryopreservation method using glycerol as a cryoprotector.
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Criopreservação , Espermatozoides , Fecundação , Ovários , Reprodução , Cryopreservation , Sperm , Fertilization , Ovary , Reproduction
Citação
ROCHA, J. P. Cultivo de embriões bovinos em estágio de blastocisto em meio sequencial, após maturação e fertilização de oócitos in vitro, de novilhas e vacas velhas: comparação entre métodos de congelamento lento, computadorizado e ultra-rápido. 2004. 30 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2004.