Análise do secretoma do fungo Trichoderma harzianum crescido em presença de glicose ou parede celular de Fusarium solani
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2010-03-31
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Universidade Federal de Goiás
Resumo
Trichoderma harzianum is a saprophytic fungus, known for its potential as
a biological control agent of different phytopathogens that causes losses in
crops. Its action is based on different mechanisms like volatile and non-volatile
antibiotics production, competition for nutrient and space, production of
hydrolytic enzymes and mycoparasitism. Fusarium solani is the agent of bean
dry root rot, and is responsible for significant losses. This work sought to
analyze and identify secreted proteins from T. harzianum grown in the presence
of F. solani cell wall, in an attempt to obtain new insights on biocontrol.In the
dual culture test, T. harzianum proved to be a potent antagonist of F. solani.
Some differences in the secreted proteins profile and final medium pH were
observed when T. harzianum was grown in TLE medium containing glucose
and in TLE and Minimum medium containing the cell wall of the phytopathogen.
33 proteins from 18 different genes were identified. The protein Sm1 was
identified and secreted in all growth conditions. Many hydrolytic enzymes
related to mycoparasitism, like endochitinases, β-1,3, β-1,6-glucanases,
proteases and α-1,3-glicanases, were identified and among the proteases, a
hypothetical metalocarboxipeptidase and a serine subtilisin-like never described
for T. harzianum. Some enzymes with unkown roles in the mycoparasitism,
such as β-1,3-glucanosyltransferase, secreted only in MM growth medium
supernatant, were also identified.
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RAMADA, Marcelo Henrique Soller. Analysis of the secretome of Trichoderma harzianum grown in the presence of glucose or cell walls of Fusarium solani. 2010. 104 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2010.