Avaliação do potencial do peptídeo indolicidina na descontaminação de sêmen bovino
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2021-03-04
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Universidade Federal de Goiás
Resumo
The growing interest in artificial insemination in cattle has led researchers to study techniques
that make it possible to preserve semen without compromising its ability to fertilize. This
project evaluated the antiviral and antibacterial activity of the peptide indolicidin and its
potential use as an antiviral agent for a product of high commercial value, which is the semen
of selected cattle. Firstly, the antibacterial activity of the indolicidin peptide was evaluated
against the bacterium E. coli at the concentrations described in the literature. Based on
Escherichia coli, the antibacterial activity of the peptide was evaluated at concentrations of 10
μM, 20 μM and 30 μM against Leptospira interrogans. Subsequently, the peptide was tested at
concentrations of 10 μM, 20 μM and 30 μM on cultured cells (MDBK), in which no changes
in cell morphology or viability were observed. The antiviral activity of the peptide was then
analyzed in MDBK cells artificially infected with bovine alphaherpesvirus type-1 (BoHV-1),
in which the peptide was incubated at concentrations of 4 μM, 6 μM, 8 μM, 10 μM, 20 μM and
30 μM, and the safety of the peptide against bovine semen was evaluated according to quality
parameters. The indolicidin peptide was safe against semen and cultured cells. In bacteria, the
best concentration identified was 30 μM, while in BoHV-1 the best concentration was 8 μM.
To date, no methodology has been described for the efficient inactivation of BoHV-1, nor even
for minimizing the viral concentration in bovine semen, which makes this project interesting
for the commercialization of semen on the national and international markets.
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SANTOS, G. H. Avaliação do potencial do peptídeo indolicidina na descontaminação de sêmen bovino. 2021. 45 f. Dissertação (Mestrado em Ciência Animal) - Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, 2021.