Efeito do aumento de temperatura na diversidade filogenética da microbiota planctônica acessada por metabarcode

Abstract

There are estimates of an increase of up to 2.5°C in the average global temperature by the end of this century, making the study through experimentation an important tool for observing the behavior of organisms in the face of warming. Using Metabarcoding techniques, we can extract relevant information from the data resulting from experiments, analyzing the phylogenetic diversity of species. Our experiment used 20 aquariums with heaters to simulate warming scenarios, with 4 treatments: Control treatment; Intermediate treatment with warming of 2°C above the average; pessimistic treatment with warming of 4°C above the average; and pessimistic-pessimistic treatment with an increase of up to 8°C. To observe the differences in phylogenetic diversity between treatments, the phylogeny of organisms was constructed, the phylogenetic indices were estimated and an anova was performed. There were significant differences for less, only in the MNPD index (average distance of the nearest neighbor), of the Intermediate-Control treatments; being general eukaryotes with a P value = 0.02 and for classified eukaryotes with a P value = 0.04. The results demonstrate little change in the composition of species in the face of warming. The diversity metrics used here can contribute to species conservation planning by revealing information about their phylogenetic characteristics