Análise proteômica comparativa do processo de diferenciação celular do fungo patogênico Paracoccidioides brasiliensis
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2014-07-31
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Universidade Federal de Goiás
Resumo
Paracoccidioides spp. is the etiological agent of paracoccidioidomycosis, the most important
endemic systemic mycosis in Latin America. Paracoccidioides spp. is a dimorphic fungus;
mycelia is found in soil at temperatures below 25ºC, while in host tissues, and temperature
around 36-37ºC the fungus takes the yeast form. Infection begins with the inhalation of
conidia or mycelia propagules that upon reaching the host lungs differentiate into yeast,
establishing disease. The morphological transition from mycelia-to-yeast is involved in the
virulence of this pathogen and this aspect of morphogenesis deserves special attention due to
its relevance to the fungal virulence. In the present study, we employed proteomic strategies
using liquid chromatography coupled to mass spectrometry to evaluate the differential
proteomic profile of cells ongoing transition from mycelia-to-yeast after 22 h of temperature
shift from 22ºC to 36ºC (P. brasiliensis Pb-18 phylogenetic lineage S1). Nine hundred and
ninety-one proteins were identified (350 in the mycelia, 288 in the transition and 353 in the
yeast), and 251 were differentially regulated. The analysis of the functional categories to
which those proteins belong provided us a comprehension on the metabolic reprogramming
that occurs during the cell differentiation process, providing putative virulence factors.
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VAZ, A. F. Análise proteômica comparativa do processo de diferenciação celular do fungo patogênico Paracoccidioides brasiliensis. 2014. 124 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2014.