Determinação da imunogenicidade de proteínas de Mycobacterium bovis isolados de bovinos naturalmente infectados no Estado de Goiás

Abstract

The purpose of this work is to contribute to the research in new diagnostic tests for bovine tuberculosis. To this end a study was performed to identify Mycobacterium bovis immunogenic proteins isolated from bovines in the field. Total extracted protein, were initially fractioned by electrophoresis in a poliacrylamide gel and then identified by Western Blotting, using serum from reagent and non-reagent animals to the purified protein derivate (PPD) intradermal diagnostic test. Protein immunogenicity was assessed according to the molecular weight profile of the positive bands. 93 serum samples were tested from PPD positive and PPD negative animals from Goiás, Distrito Federal and Minas Gerais, as well as tuberculosis free regions of the USA. By means of the Western Blotting technique it was possible to identify the 26kDa protein as the most immunogenic among the positive PPD tested animals.