Ação citotóxica e antioxidante da beta lapachona em células endoteliais
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2017-12-18
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Universidade Federal de Goiás
Resumo
Oxidative stress is the overproduction of reactive oxygen species that cause cell damage and
even the death. It is a mechanism related to ischemia and reperfusion, a process that affects
several organs and is involved in neurodegenerative diseases, cardiac and/or renal
insufficiencies, hepatic dysfunction, among others. The discovery of new antioxidant substances
derived from natural sources, is important for the prevention and treatment of these diseases.
Beta lapachone is a substance with antioxidant action obtained from sawdust of Ipe wood, a
symbolic tree from the Brazilian cerrado. This study aimed to analyze the cytotoxic and
antioxidant action of beta lapachone in endothelial cells, in vitro. EA.hy 926 lineage cells were
subcultured, treated with different concentrations of beta lapachone and subjected to ischemia
and reperfusion. Cytotoxicity and antioxidant action were assessed through the cellular viability
analysis by the tetrazolium reduction method. The averages were verified by the Tukey test (5%
of significance) and by variance analysis. There was a dose-dependent progression of
cytotoxicity in the non-ischemia/reperfusion (control) group and cell viability increase in the
ischemia/reperfusion group. However, there was no statistical difference between the
concentrations and between groups. Considering the settings of this experiment, Beta
lapachone did not present cytotoxic or antioxidant action on endothelial cells.
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GONÇALVES, P. A. M. Ação citotóxica e antioxidante da beta lapachona em células endoteliais. 2017. 51 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017.