Determinação de escopolamina e butilescopolamina em amostras de bebidas, urina e comprimidos de Buscopan® usando microssistemas eletroforéticos
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2023-08-08
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Universidade Federal de Goiás
Resumo
The number of cases in which scopolamine (SCO) was used for both
recreational and predatory purposes has increased dramatically in recent
decades. Linked to this, there is a concern about obtaining SCO through
thermal degradation of butylscopolamine (BSCO) – an active ingredient of
Buscopan® – a drug sold without a medical prescription. In this study, mixtures
containing SCO and BSCO were separated and detected on a microchip
electrophoresis (ME) device with integrated capacitively coupled contactless
conductivity detection (C4D) using a running buffer composed of 40 mmol L-1
of butyric acid and 25 mmol L-1 of sodium hydroxide (pH 5.0). The separation
was performed within ca. 115 s with a resolution of 1.3 and separation
efficiency of 1.4x105 and 1.5x105
theoretical plates m-1
for SCO and BSCO,
respectively. The limit of detection was 1.1 μmol L-1
for both species and the
developed method revealed satisfactory repeatability with relative standard
deviation (RSD) values for forty-eight injections between 4.8 and 9.4% for peak
areas and lower than 3.3% for migration times. Furthermore, inter-day
precision was evaluated for sixteen injections (a sequence of four injections
performed over four days), and RSD values were less than 6.6% for peak
areas and 2.2% for migration times. Satisfactory recovery values (95 – 114 %)
were obtained for all evaluated beverage samples (cachaça, vodka, whiskey,
beer, coke soft drink, and grape juice) as well as for artificial urine samples (95
– 107 %). Finally, the conversion of BSCO into SCO was observed after simple
heating procedure of Buscopan® sample (not subject to medical prescription),
which was successfully confirmed through analysis by capillary
electrophoresis coupled to the mass spectrometry (CE-MS). Based on the
reported results, the use of ME-C4D devices has demonstrated a huge
potential for applications in the forensic chemistry field.
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Citação
SANTOS, H. I. Determinação de escopolamina e butilescopolamina em amostras de bebidas, urina e comprimidos de Buscopan® usando microssistemas eletroforéticos. 2023. 88 f. Dissertação (Mestrado em Química) - Instituto de Química, Universidade Federal de Goiás, 2023.