Spray de quitosana na cicatrização de feridas cutâneas em ratas diabéticas
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Data
2016-08-02
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Universidade Federal de Goiás
Resumo
The healing is a complex and dynamic event aimed at the restoration of tissue integrity.
However, some factors, such as diabetes, can harm this physiological event. Chitosan, a
derivative of chitin, has been considered as a versatile and effective option for the treatment
of wounds. This study aimed to evaluate and compare the effect of chitosan 2% gel and
chitosan spray based on secondary intention healing of skin wounds in diabetic rats. 54 rats
were used, divided randomly into three groups: control group (CG), chitosan 2% gel group
(GG), and Chitosan spray group (GS) with 18 animals each. The rats in GC, GG, and GS had
their wounds treated daily with saline solution 0.9%, chitosan 2% gel, and chitosan spray,
respectively. The groups were divided into three subgroups (n = 6) according to the times of
histological analysis of wound (third, seventh, and 14th day). The induction of diabetes in the
rats took place by intraperitoneal application of alloxan (120 mg/kg). After confirming the
state of diabetes, the animals were anesthetized and the wounds made with the aid of a punch
9.0mm in the dorsal region. Macroscopic and microscopic analyzes were performed.
Macroscopic analysis showed that after 14 days, the chitosan 2% gel and chitosan spray gave
a contraction rate of 100% and 99%, respectively. At the histological level, there was the
anticipated recruitment of mononuclear cells, fibroblasia, and deposition of dense
fibrovascular tissue and marked fibrosis in the wounds of GG and GS, emphasizing the ability
of this biopolymer to optimize the healing of diabetics. It can therefore be a therapeutic option
for the development of other formulations that provide wound repair in diabetic patients.
Descrição
Palavras-chave
Curativo , Diabetes , Hidrogel , Polissacarídeo , Quitina , Curative , Diabetes , Hydrogel , Polysaccharide , Chitin
Citação
SANTOS, T. C. C. Spray de quitosana na cicatrização de feridas cutâneas em ratas diabéticas. 2016. 49 f.
Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.