Análise de populações de Sclerotinia sclerotiorum em cultura de feijoeiro através de marcadores SSR.
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2009-09-30
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Universidade Federal de Goiás
Resumo
79 Sclerotinia sclerotiorum isolates were collected under central pivot irrigations
sistems, from Brazilian fields, divided in 4 populations (S2, S3, A and M) and analyzed
to determine the genetic variability among and between populations using molecular
markers based on microsatelittes. 10 primers were used and the amplification products
were separate in poliacrilamida gels and the bands were silver stained. A total of 102
different haplotypes were identified, and the amount of haplotypes varied from 6 to 18
for locus. The genotypic diversity ranged from 65% to 91%. Analyses based on genetic
diversity and fixation indices which indicate the variability between populations was
28.79% (FST = 28793) and the variability among populations was 71.21%. The Jaccard
similarity index indicated that the populations S2 and A is genetically closer. The
population S3 presented a similarity index of 0.44 compared with the populations S2 and
A. The population M, originated from several collection sites, was considered
genetically more distant showing a index of 0.46 compared with the others populations .
The high variability between and among populations can indicate that, besides the
possible introduction of new genotypes in the analyzed fields, could be having clonal
and sexual reproduction in isolated of S. sclerotiorum from Brazilian cerrado.
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Citação
GOMES, Eriston Vieira. Analysis of populations of Sclerotinia sclerotiorum in bean cultivation by
SSR markers.. 2009. 56 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2009.