Cienciometria em Entomogenética e recuperação de DNA Humano do sistema gastrointestinal de Diptera

Abstract

Data presented by the Brazilian Yearbook of Public Security (2024), points to the increasing cases of rape in Brazil. Scientific research has developed tools and techniques with high investigative potential, which have helped the justice system materialize evidence. Animal models are widely used in forensics, such as estimating the post-mortem interval. Entomogenetics, which is a sub-area of forensic entomology, has a high potential in cases of DNA recovery from the immature insects pappus of flies for the elucidation of crimes. Molecular biology tools have been applied both in the recovery of victims in advanced stages of decomposition and in the identification of aggressors in cases of sexual crimes followed by death. This thesis was structured in two chapters, the first chapter aimed to recover and isolate human DNA from the contents present in the gastrointestinal region of immature insects of a fly species from the Sarcophagidae family. Thus, conventional PCR and qPCR techniques were used. In addition, the Barcode DNA technique was used to identify the species of fly. The qPCR technique proved effective in isolating and detecting human DNA and male genetic material from the gastric system of the larvae of flies from the Sarcophagidae family. This approach was innovative and was applied for the first time with the aim of detecting human target DNA in samples from the contents stored in the stomach of larvae and pupae of flies from the family studied. Using the DNA Barcode technique, the Peckia resona species was identified, providing new perspectives for the fields of taxonomy and entomology. The second chapter, write as article, aimed to carry out a scientometric analysis of all scientific productions in the field of entomogenetics. This study offered insights into the evolution of entomogenetics-related publications over the years and provided a comprehensive understanding of the use of molecular biology methodologies to recover DNA from the digestive system of insects, the number of articles, the pioneering authors who contributed to this field, and the existing gaps in knowledge. The data revealed a scarcity of research on entomogenetics, highlighting the need to promote investigations in this area by conducting studies in different countries.