Inferência de micrornas candidatos a influenciar a expressão do gene imunosupressor HLA-G
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Data
2014-02-19
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Universidade Federal de Goiás
Resumo
MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional
expression regulation by inducing mRNA degradation or translation inhibition. Some
miRNAs are known to regulate HLA-G expression, an important immunemodulatory
molecule that inhibits both Natural Killer and cytotoxic T cells through interaction with
inhibitory receptors. The HLA-G is associated with maternal-fetal tolerance, tissue
acceptance in transplants and the progression of tumors. The mechanisms
underlying HLA-G expression control are not completely understood, however, its
3’untranslated region (3’UTR) is reported to play an important role on gene regulation
influencing mRNA stability and interacting with miRNAs such as miR-148a-3p. In this
study, we performed a systematic analysis of all miRNAs that are good candidates to
act as HLA-G regulators. In order to determine the miRNAs with the highest potential
to influence HLA-G expression, we compared the outputs of three distinct algorithms
- miRanda, RNAhybrid and Pita. For this purpose, a method of miRNA inference was
developed using Perl scripts to compare and filter results and a scoring system was
created in order to evaluate both the binding stability of the miRNA/mRNA interaction
and the miRNA specificity to its target sequence. Then, a panel of miRNAs with great
potential of controlling HLA-G expression was generated.
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PORTO, I. O. P. Inferência de micrornas candidatos a influenciar a expressão do gene imunosupressor HLA-G. 2014. 55 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2014.