In vitro evaluation of Bacillus licheniformis protease on the nematodes Panagrellus sp., Meloidogyne incognita, Haemonchus spp. and Trichostrongylus spp

Abstract

The aim of this study was to evaluate the in vitro action of the protease (HPF) from Bacillus licheniformis on the nematodes Panagrellus sp., Meloidogyne incognita, Haemonchus spp., and Trichostrongylus spp. Eight experimental groups were formed, one control group (distilled water), and seven groups treated with approximately 60 ne- matodes + HPF. There was a significant reduction ( p < 0.01) in Panagrellus sp. and M. incognita for all tested concentrations (0.100, 0.147, 0.215, 0.316, 0.467, 0.681 and 1.00 % w/v). However, for gastrointestinal nema- todes (GINs), reduction was observed only at concentrations of 0.464 % (w/v), 0.681 %(w/v), and 1.00 %(w/v). The reduction percentages reached 100 ± 0.0 % for M. incognita , 76 ± 1.6 % for Panagrellus sp., and 54 % ± 2.9 for the GINs.