Navegando por Autor "André, Maria Cláudia Dantas Porfirio Borges"
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Item Antimicrobial activity of copaifera spp. against bacteria isolated from milk of cows with mastitis(Universidade Federal de Goiás, 2017-03-03) Souza, Maria Juíva Marques de Faria; Silva, Carla Afonso da; Paula, José Realino de; André, Maria Cláudia Dantas Porfirio Borges; Vaz, Boniek Gontijo; Carvalho, Thays Colletes De; Romão, Wanderson; Costa, Helber Barcellos da; Conceição, Edemilson Cardoso daThe antimicrobial activities of the oleoresin (OR) and the essential oil (EO) of Copaifera spp. were checked against microorganisms isolated from milk samples of cows diagnosed with grade III subclinical mastitis. The OR had good antimicrobial activity (MIC ≤ 100 µg/mL) against samples of coagulase-positive Staphylococcus, coagulase-negative Staphylococcus, Streptococcus of groups C, F, and G, and Corynebacterium spp. Meanwhile, the EO had good antimicrobial activity (MIC≤100 µg/mL) against coagulase-negative Staphylococcus and Corynebacterium spp. The OR and the EO were inactive and weak to inactive, respectively, against Escherichia coli. Overall, the OR had better antimicrobial activity than the essential oil against the 55 bacterial isolates (p<0.0001). The GC/MS analysis identified sesquiterpenes in EO and by the ESI FT-ICR MS method, the identification of diterpenic acids in OR was possible. Therefore, this raw plant material is promising for the development of phytotherapeutic drugs against bovine mastitis.Item Hygienic, sanitary, physical, and functional conditions of Brazilian public school food services(2014-06) Almeida, Kénia Machado de; André, Maria Cláudia Dantas Porfirio Borges; Campos, Maria Raquel Hidalgo; Piscoya Diaz, Mario ErnestoObjective To verify the physical, functional, hygienic, and sanitary conditions of the food services of municipal schools located in the Brazilian Midwest region. Methods This is a cross-sectional study of 296 school food services conducted from February to June 2012. The food services were assessed by a semi-structured check list divided into the following sections: physical conditions, available equipment, food handlers’ conduct, and food service cleaning processes and procedures. The study variables were classified as compliant or noncompliant with the regulations passed by the National Sanitary Surveillance Agency. Results Noncompliances were found in all study food services, especially with respect to food service conditions, and the wiring and plumbing in the food preparation area. In this section, 62.7 to 95.9% of the food services did not comply with nine out of the thirteen study items. The main problems were: poorly cleaned external areas, deteriorated walls, floors, ceilings, roofs, drains, and roof gutters; and unscreened doors and windows, allowing the entrance of insects; among others. The main noncompliance regarding processes and procedures was the uncontrolled temperature of the ready-to-eat foods.Item Identificação e teste de susceptibilidade a drogas de cepas de Mycobacterium tuberculosis isoladas de pacientes provenientes de Hospital Público de Referência, Goiânia/GO(2003) Rodrigues, Márcia Alves Vasconcelos; André, Maria Cláudia Dantas Porfirio Borges; Alves, Suely Lemes de; Souza, Marieta Pereira M.; Kipnis, André; Serafini, Álvaro BisolTo identify and conduct susceptibility test of Mycobacterium tuberculosis strains, 206 clinical samples were isolated from pulmonary and extra-pulmonary forms, originating from 144 in- and out-patients of the Tropical Diseases Hospital from the Goiás State, by undergo Ziehl-Neelsen stain, cultures performed by the Lowenstein-Jensen and double phase system. The identification was based on growth in the presence of inhibiting agents, pigment production, growth time and biochemical testing, followed by the determination of susceptibility to anti-mycobacterial drugs. Thirteen samples (6.3%) were isolated, which 12 (92.3%) were identified as M. tuberculosis and one (7.7%) as M. avium Complex (MAC). From the isolated samples, 12 were sensitive to isoniazid, ethanbutol, rifampicin, streptomycin, pirazinamid and ethionamid. Only one sample was resistant to ioniazid, rifampicin, pi-razinamid and ethionamid.Item Methicillin-resistant Staphylococcus aureus nasal carriage in neonates and children attending a pediatric outpatient clinics in Brazil(2014-02) Vieira, Maria Aparecida; Minamisava, Ruth; Pessoa Junior, Vicente Porfirio; Cardoso, Juliana Lamaro; Ternes, Yves Mauro Fernandes; André, Maria Cláudia Dantas Porfirio Borges; Andrade, Sabrina Sgambatti de; Kipnis, André; Andrade, Ana Lúcia Sampaio Sgambatti deBackground: In Latin America, few studies have been carried out on methicillin-resistant Staphylococcus aureus carriage in the pediatric population. We conducted a survey of nasal S. aureus carriage in neonates and in children attending the pediatric outpatient clinics in a large Brazilian city with high antimicrobial consumption. Methods: Pernasal swabs of neonates were collected upon admission and at discharge in four neonatal intensive care units and of children less than five years of age during outpatient visits. Methicillin-resistant S. aureus isolates were characterized for antibiotic susceptibility, mec gene presence, pulsed-field gel electrophoresis, spatype, SCCmec-type, multilocus sequence type, and presence of Panton-Valentine leukocidin genes. Results: S. aureus was carried by 9.1% and 20.1% of the 701 neonates and of 2034 children attending the outpatient clinics, respectively; methicillin-resistant S. aureus carriage was detected in 0.6% and 0.2%, of the these populations, respectively. Healthcare-associated methicillin-resistant S. aureus strains found in neonates from neonatal intensive care units and outpatients were genetically related to the Brazilian (SCCmec-III, ST239) and to the Pediatric (SCCmec-IV, ST5) clones. Community-associated methicillin-resistant S. aureus was only detected in outpatients. None of the methicillin-resistant S. aureus strains contained the Panton-Valentine leukocidin gene. Methicillin-resistant S. aureus strains related to the Brazilian clone showed multidrug resistance pattern. Conclusions: Despite the high antibiotic pressure in our area, and the cross transmission of the healthcare-associated methicillin-resistant S. aureus clones between neonatal intensive care units and outpatients, the prevalence of methicillin-resistant S. aureus carriage is still low in our setting.Item Methicillin-resistant Staphylococcus sp. colonizing health care workers of a cancer hospital(2014-09) Costa, Dayane de Melo; Kipnis, André; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Vilefort, Larissa Oliveira Rocha; Teles, Sheila Araujo; André, Maria Cláudia Dantas Porfirio Borges; Tipple, Anaclara Ferreira Veiga; Lima, Ana Beatriz Mori; Ribeiro, Nádia Ferreira Gonçalves; Pereira, Mayara Regina; Prado, Marinésia Aparecida doThe aim of the study was to analyze epidemiological and microbiological aspects of oral colonization by methicillin-resistant Staphylococcus of health care workers in a cancer hospital. Interview and sa- liva sampling were performed with 149 health care workers. Antimicrobial resistance was deter- mined by disk diffusion and minimum inhibitory concentration. Polymerase Chain Reaction, Internal Transcribed Spacer-Polymerase Chain Reaction and Pulsed Field Gel Electrophoresis were per- formed for genotypic characterization of methicillin-resistant Staphylococcus. Risk factors were de- termined by logistic regression. Methicillin-resistant Staphylococcus colonization prevalence was 19.5%, denture wearing (p = 0.03), habit of nail biting (p = 0.04) and preparation and administration of antimicrobial (p = 0.04) were risk factors identified. All methicillin-resistant Staphylococcus were S. epidermidis, 94.4% of them had mecA gene. Closely related and indistinguishable methicillin- resistant S. epidermidis were detected. These results highlight that HCWs which have contact with patient at high risk for developing infections were identified as colonized by MRSE in the oral cavity, reinforcing this cavity as a reservoir of these bacteria and the risk to themselves and patients safety, because these microorganisms may be spread by coughing and talking.Item Molecular characterization of Listeria monocytogenes isolated from animal products in a city of northern Brazil(2013-08) Monteiro, Lilyan Rosmery Luizaga de; Mesquita, Albenones José de; André, Maria Cláudia Dantas Porfirio Borges; Cardoso, Juliana LamaroListeria monocytogenes, a foodborne pathogen causes listeriosis, a fatal disease in about 30% of cases that affects mainly immunocompromised persons. The aim of this research was to characterize L. monocytogenes pulsed-fi eld gel electrophoresis (PFGE) types isolated from meat products collected at public markets in Araguaina city, TO. Sixty samples of raw ground beef and frescal sausage were analyzed during the second half of 2008. Five out of 30 samples (16.7%) of raw ground beef tested positive for L. monocytogenes, three of which were classifi ed as serotype 1/2b and two as serotype 4b. Among the 30 samples of sausage collected, two strains of L. monocytogenes were isolated (6.7%), one of them belonging to serotype 1/2a and the other belonging to serotype 1/2b. The restriction enzymes used were ApaI and SmaI. Similarities among the strains were determined by Dice coeffi cient. The macro restriction profi le obtained by using SmaI enzyme allowed the distribution of seven strains in two clusters, two pulsotypes and two subtypes. The result indicates that L. monocytogenes isolates, belonging to serotype 4b, 1/2a and 1/2b, are strongly correlated within the same serotype group, and in some cases among different serotypes, suggesting that they have a common source.Item Pseudomonas spp. isolated from the oral cavity of healthcare workers from an oncology hospital in midwestern Brazil(2015-12) Lima, Ana Beatriz Mori; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Costa, Dayane de Melo; Vilefort, Larissa Oliveira Rocha; André, Maria Cláudia Dantas Porfirio Borges; Barbosa, Maria Alves; Prado, Marinésia Aparecida doThis cross-sectional study, performed in an oncology hospital in Goiania, aimed to characterize the prevalence of oral colonization and antimicrobial susceptibility of Pseudomonas spp. isolated from the saliva of healthcare workers. Microorganisms were subjected to biochemical tests, susceptibility profile, and phenotypic detection. Of 76 participants colonized with Gram negative bacilli, 12 (15.8%) harbored Pseudomonas spp. Of all isolates, P. aeruginosa (75.0%), P. stutzeri (16.7%), and P. fluorescens (8.3%), were resistant to cefoxitin, and therefore likely to be AmpC producers. The results are clinically relevant and emphasize the importance of surveillance to minimize bacterial dissemination and multiresistance.Item Utilização do antibiograma como ferramenta de tipagem fenotípica de Staphylococcus aureus isolados de manipuladores, leite cru e queijo minas frescal em laticínio de Goiás, Brasil(2006) André, Maria Cláudia Dantas Porfirio Borges; Santos, Patrícia Pimentel; Campos, Maria Raquel Hidalgo; Borges, Liana Jayme; Serafini, Álvaro BisolDuring a year (March/2004 to February/2005) 140 samples of raw milk, dairy staff, and Minas Frescal cheese were analyzed for the presence of S. aureus in a dairy plant in Goiás, Brazil. Strains were isolated from 75% of the personnel investigated resulting in 31 (33.7%) isolates from 92 collected samples (46 from hands and 46 from nasopharynx). The 24 raw milk samples resulted in 18 (75.0%) positive samples and 26 isolates, with mean number: 1.1 x 10 5 CFU/ml. Among 24 cheese samples 17 (70.8%) were positive resulting in 20 isolates, with mean number: 3.8 x 104 CFU/g. Thirteen samples (54.2%) were above the limit established by the brazilian legislation (10 3 CFU/g). The differentiation of strains was performed by antimicrobial susceptibility test. All isolates were susceptible to ciprofloxacin and gentamicin. Resistance was observed in five (6.5%) isolates for erythromycin, 19 (24.7%) for tetracycline, one (1.3%) for vancomycin, four (5.2%) for oxacillin and 53 (68.8%) isolates for penicillin. Eleven isolates (14.3%) were susceptible to all antibiotics tested and 18 (23.4%) were resistant to more than one antibiotic. The antibiotic susceptibility allowed the discrimination of isolates in 12 different profiles (A-L), but the phenotypic typing failed to determine the source of bacteria for the cheese.