Molecular characterization of Listeria monocytogenes isolated from animal products in a city of northern Brazil
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2013-08
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Resumo
Listeria monocytogenes, a foodborne pathogen
causes listeriosis, a fatal disease in about 30% of cases that affects
mainly immunocompromised persons. The aim of this research was
to characterize L. monocytogenes pulsed-fi eld gel electrophoresis
(PFGE) types isolated from meat products collected at public
markets in Araguaina city, TO. Sixty samples of raw ground beef
and frescal sausage were analyzed during the second half of 2008.
Five out of 30 samples (16.7%) of raw ground beef tested positive
for L. monocytogenes, three of which were classifi ed as serotype
1/2b and two as serotype 4b. Among the 30 samples of sausage
collected, two strains of L. monocytogenes were isolated (6.7%),
one of them belonging to serotype 1/2a and the other belonging
to serotype 1/2b. The restriction enzymes used were ApaI and
SmaI. Similarities among the strains were determined by Dice
coeffi cient. The macro restriction profi le obtained by using SmaI
enzyme allowed the distribution of seven strains in two clusters,
two pulsotypes and two subtypes. The result indicates that L.
monocytogenes isolates, belonging to serotype 4b, 1/2a and 1/2b,
are strongly correlated within the same serotype group, and in
some cases among different serotypes, suggesting that they have
a common source.
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Palavras-chave
Pulsed field gel electrophoresis, L. monocytogenes, Ground beef, Frescal sausage
Citação
MONTEIRO, Lilyan Rosmery Luizaga de; MESQUITA, Albenones José de; ANDRÉ, Maria Cláudia Dantas Porfírio Borges; CARDOSO, Juliana Lamaro. Molecular characterization of Listeria monocytogenes isolated from animal products in a city of northern region of Brazil. Ciência Rural, Santa Maria, v. 43, n. 8, p. 1443-1448, Aug. 2013.