Navegando por Autor "Lima, Ana Beatriz Mori"
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Item Bastonetes Gram-negativos em úlceras venosas e implicações para o atendimento de enfermagem na atenção primária(Marcelo Medeiros, 2014-06-30) Santos, Silvana de Lima Vieira dos; Martins, Marlene Andrade; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Lima, Ana Beatriz Mori; Malaquias, Suelen Gomes; Bachion, Maria MárciaA descriptive cross-sectional study in patients with hard-to-heal venous ulcers, whose objective is to identify the prevalence of Gram-negative rods (GNR) in venous ulcers presenting clinical signs of infection; analyze the susceptibility profile of such ulcers and detect ESBL, AmpC and metallo-β-lactamase production. We identified 69 individuals. The samples were gathered by means of an interview, clinical exam, photographic record and a swab of the lesions. Specimens were submitted to laboratorial evaluation for microbiological analysis. A total of 98 lesions were evaluated, of which BGN presented in 74.5%. There was a prevalence of P. aeruginosa and Escherichia coli. The mircoorganisms were sensitive to most antimicrobials. In terms of resistance, tetracycline and cefoxitine were discarded. ESBL was not detected; AmpC was verified in 37.5% of the CESP group and metalo-β-lactamase in 8.0% of P. aeruginosa. Our study points to the need for microbiological vigilance of patients with hard-to-heal venous ulcers.Item Detecção de metalo-beta-lactamase em Pseudomonas aeruginosa isoladas de pacientes hospitalizados em Goiânia, Estado de Goiás(2009-08) Gonçalves, Diana Christina Pereira Santos; Lima, Ana Beatriz Mori; Leão, Lara Stefania Netto de Oliveira; Carmo Filho, José Rodrigues do; Pimenta, Fabiana Cristina; Vieira, José Daniel GonçalvesPseudomonas aeruginosa is a bacterium frequently isolated from hospital environments. This study had the aims of evaluating the susceptibility profile of Pseudomonas aeruginosa previously isolated from patients in a hospital in Goiânia (Goiás, Brazil), performing phenotypic screening for metallo-beta-lactamase production and detecting its genes using the polymerase chain reaction technique. Seventy-five 75 Pseudomonas aeruginosa isolates were evaluated between January 2005 and January 2007. Biochemical identification was performed using the API 20E® system and an antibiogram was produced using the Kirby-Bauer method. Among the 62 isolates that were resistant to imipenem and ceftazidime, 35 (56.4%) produced metallo-beta-lactamase, while 26 (74.3%) showed the blaSPM-1 gene. The frequency of Pseudomonas aeruginosa that produces metallo-beta-lactamase suggests that greater control over the dissemination of resistance in hospital environments is needed.Item Enterobacteriaceae isolates from the oral cavity of workers in a brazilian oncology hospital(2015-04) Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Lima, Ana Beatriz Mori; Costa, Dayane de Melo; Vilefort, Larissa Oliveira Rocha; Oliveira, Ana Claúdia Alves de; Gonçalves, Nádia Ferreira; Vieira, José Daniel Gonçalves; Prado, Marinésia Aparecida doThe evaluation of workers as potential reservoirs and disseminators of pathogenic bacteria has been described as a strategy for the prevention and control of healthcare-associated infections (HAIs). The aim of this study was to evaluate the presence of Enterobacteriaceae in the oral cavity of workers at an oncology hospital in the Midwest region of Brazil, as well as to characterize the phenotypic profile of the isolates. Saliva samples of 294 workers from the hospital’s healthcare and support teams were collected. Microbiological procedures were performed according to standard techniques. Among the participants, 55 (18.7%) were colonized by Enterobacteriaceae in the oral cavity. A total of 64 bacteria were isolated, including potentially pathogenic species. The most prevalent species was Enterobacter gergoviae (17.2%). The highest rates of resistance were observed for β-lactams, and 48.4% of the isolates were considered multiresistant. Regarding the enterobacteria isolated, the production of ESBL and KPC was negative. Nevertheless, among the 43 isolates of the CESP group, 51.2% were considered AmpC β-lactamase producers by induction, and 48.8% were hyper-producing mutants. The significant prevalence of carriers of Enterobacteriaceae and the phenotypic profile of the isolates represents a concern, especially due to the multiresistance and production of AmpC β-lactamases.Item Methicillin-resistant Staphylococcus sp. colonizing health care workers of a cancer hospital(2014-09) Costa, Dayane de Melo; Kipnis, André; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Vilefort, Larissa Oliveira Rocha; Teles, Sheila Araujo; André, Maria Cláudia Dantas Porfirio Borges; Tipple, Anaclara Ferreira Veiga; Lima, Ana Beatriz Mori; Ribeiro, Nádia Ferreira Gonçalves; Pereira, Mayara Regina; Prado, Marinésia Aparecida doThe aim of the study was to analyze epidemiological and microbiological aspects of oral colonization by methicillin-resistant Staphylococcus of health care workers in a cancer hospital. Interview and sa- liva sampling were performed with 149 health care workers. Antimicrobial resistance was deter- mined by disk diffusion and minimum inhibitory concentration. Polymerase Chain Reaction, Internal Transcribed Spacer-Polymerase Chain Reaction and Pulsed Field Gel Electrophoresis were per- formed for genotypic characterization of methicillin-resistant Staphylococcus. Risk factors were de- termined by logistic regression. Methicillin-resistant Staphylococcus colonization prevalence was 19.5%, denture wearing (p = 0.03), habit of nail biting (p = 0.04) and preparation and administration of antimicrobial (p = 0.04) were risk factors identified. All methicillin-resistant Staphylococcus were S. epidermidis, 94.4% of them had mecA gene. Closely related and indistinguishable methicillin- resistant S. epidermidis were detected. These results highlight that HCWs which have contact with patient at high risk for developing infections were identified as colonized by MRSE in the oral cavity, reinforcing this cavity as a reservoir of these bacteria and the risk to themselves and patients safety, because these microorganisms may be spread by coughing and talking.Item Perfil dos trabalhadores de um hospital oncológico colonizados na cavidade bucal por enterobacteriaceae(Ruy de Souza Lino Junior, 2014-08-13) Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Lima, Ana Beatriz Mori; Costa, Dayane de Melo; Rocha-Vilefort, Larissa Oliveira; Oliveira, Ana Cláudia Alves de; Vieira, José Daniel Gonçalves; Prado, Marinésia Aparecida do,The aim of this study was to characterize the profile of workers of the oncology hospital colonized by Enterobacteriaceae in the oral cavity. Along with bacterial colonization, socio-demographic, professional, disease/ infection and behavioral variables were investigated. The collection of saliva and microbiological analyses were performed using standard techniques. Data collection was conducted through a questionnaire. Among the 55 professionals colonized by Enterobacteriaceae, 56.4% (31/55) also harbored Staphylococcus and/or Pseudomonas in the oral cavity. The most commonly infected professionals were nursing technicians. Episodes of disease/infection were reported among carriers. The data revealed a worrying situation in the context of healthcare. It is hoped that these results will contribute to the prevention and control of this infection, as knowledge of carrier status reduces the risk of microorganism transmission.Item Prevalência de bastonetes Gram-negativos isolados da saliva de trabalhadores da saúde(Marcelo Medeiros, 2011-12) Prado-Palos, Marinésia Aparecida; Gir, Elucir; Lima, Ana Beatriz Mori; Leão, Lara Stefânia Netto de Oliveira; Pimenta, Fabiana CristinaO estudo objetivou determinar a prevalência de bastonetes Gram-negativos (BGN) isolados da saliva de trabalhadores de saúde de um hospital universitário em Goiânia-Goiás e caracterizar o perfil dos colonizados. De agosto⁄2005 a julho⁄2006, participaram do estudo 278 trabalhadores de saúde responsáveis pelos cuidados assistenciais aos clientes atendidos na instituição. A taxa de portadores foi de 69,4%, destes 44% foram considerados portadores persistentes e 49,2% multicolonizados. Foram isolados 319 BGN, sendo 208 (65,2%) enterobactérias e 111 (34,8%) não-fermentadores. As principais espécies identificadas foram: Enterobacter aerogenes (17,9%), Klebsiella pneumoniae (16,3%), Enterobacter agglomerans (8,8%). No grupo dos BGN não-fermentadores, foram isoladas 25 (7,8%) Pseudomonas aeruginosa. Elevada prevalência de trabalhadores colonizados por BGN foi observada. Os resultados obtidos neste estudo são de grande relevância, pois BGN assumem fundamental importância na etiologia e patogênese de infecções relacionadas à assistência à saúde de pacientes críticos, submetidos a procedimentos invasivos e com permanência prolongada no ambiente nosocomial. _______________________________________________________________________________________________________________________ ABSTRACT _______________________________________________________________________________________________________________________ The aim of this study was to determine the prevalence of Gram-negative (GNB) isolated from the saliva of health workers in a school hospital in Goiânia-Goiás, and to characterize the profile of the colonized individuals. From August/2005 to July/2006, 278 health care workers participated of the study. The GNB carrier rate was 69.4%, being 44% of them persistent carriers, and 49.2% multicolonized carriers. 319 BGN were isolated, of which 208 (65.2%) were Enterobacteriaceae and 111 (34.8%) non-fermenters. The main species identified were Enterobacter aerogenes (17.9%), Klebsiella pneumoniae (16.3%), and Enterobacter agglomerans (8.8%). In the group of non-fermenting GNB were isolated 25 (7.8%) Pseudomonas aeruginosa. High prevalence of colonization by GNB was observed. The results of this study are of great importance because BGN are important in the etiology and pathogenesis of infections associated with health care of critically ill patients, undergoing invasive procedures and prolonged stay in the nosocomial environment. _______________________________________________________________________________________________________________________ ABSTRACT _______________________________________________________________________________________________________________________ El objetivo del estudio fue determinar la prevalencia de bacterias Gram-negativas (BGN) aislado de la saliva de trabajadores de salud en un hospital universitario en Goiânia-Goiás y caracterizar el perfil de los colonizados. Participaron del estudio realizado entre Agosto/2005 a Julio/2006, 278 profesionales 278 responsables por asistencia sanitaria a los clientes. La tasa de portadores fue 69,4%, siendo de estos 44% persistente y 49,2% multicolónias. 319 BGN fueron aislados, 208 (65,2%) enterobacterias y 111 (34,8%) no fermentadores. Principales especies identificadas fueron Enterobacter aerogenes (17,9%), Klebsiella pneumoniae (16,3%), Enterobacter agglomerans (8,8%). En el grupo de los no fermentadores fueron aisladas 25 (7,8%) Pseudomonas aeruginosa. Alta prevalencia de colonización por los trabajadores GNB se observó. Los resultados de este estudio son de gran pertinencia, porque BGN son de fundamental importancia en la etiología y patogénesis de las infecciones relacionadas con el cuidado de salud de los pacientes enfermos, sometidos a procedimientos invasivos y estancia prolongada en ambiente hospitalario.Item Pseudomonas spp. isolated from the oral cavity of healthcare workers from an oncology hospital in midwestern Brazil(2015-12) Lima, Ana Beatriz Mori; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Costa, Dayane de Melo; Vilefort, Larissa Oliveira Rocha; André, Maria Cláudia Dantas Porfirio Borges; Barbosa, Maria Alves; Prado, Marinésia Aparecida doThis cross-sectional study, performed in an oncology hospital in Goiania, aimed to characterize the prevalence of oral colonization and antimicrobial susceptibility of Pseudomonas spp. isolated from the saliva of healthcare workers. Microorganisms were subjected to biochemical tests, susceptibility profile, and phenotypic detection. Of 76 participants colonized with Gram negative bacilli, 12 (15.8%) harbored Pseudomonas spp. Of all isolates, P. aeruginosa (75.0%), P. stutzeri (16.7%), and P. fluorescens (8.3%), were resistant to cefoxitin, and therefore likely to be AmpC producers. The results are clinically relevant and emphasize the importance of surveillance to minimize bacterial dissemination and multiresistance.