The Enterobacteriaceae family is represented by Gram negative bacillus bacteria, which can
contaminate toothbrushes. Objective: The research aimed to identify enterobacteria and to
analyze and check its plasmid resistance profile. Material and methods: The brushes were
stored in three different ways: 1/3 in a plastic brush holder, 1/3 in an open cardboard brush
holder and 1/3 in an open cardboard brush door sprayed with 1% sodium hypochlorite.
Results: After microbiological characterization, it was observed that the open cardboard
toothbrush holder had the highest number of colonies countless. Of 45 samples, seven
showed the presence of plasmid. Conclusions: This study showed that among the three forms
of packaging, the plastic and cardboard brush holders sprayed with 1% sodium hypochlorite
can be used to reduce toothbrush contamination.