Freund’s adjuvant-induced inflammation: clinical findings and its effect on hepcidin mRNA expression in horses
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2014-01
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Resumo
Hypoferremia observed during systemic inflammatory disorders is regulated by hepcidin.
Hepcidin up-regulation is particularly important during acute inflammation, as it restricts the
availability of iron, which is necessary for pathogenic microorganism growth before adaptive
immunity occurs. The aim of this study was to evaluate the clinical findings and hepatic
hepcidin mRNA expression in horses using a Freund’s complete adjuvant (FCA) model of inflammation.
The expression of hepcidin mRNA in the liver was determined in healthy horses
following two intramuscular injections of FCA at 0 h and 12 h. Plasma iron and fibrinogen
concentrations were measured at multiple time points between 0 h and 240 h post-FCA injection
(PI). Hepcidin mRNA expression was determined by RT-qPCR using liver biopsy samples
performed at 0 h (control), 6 h and 18 h PI. The mean plasma fibrinogen level was significantly
different from the control values only between 120 and 216 h PI. The mean plasma iron
level was significantly lower than the control between 16 and 72 h PI, reaching the lowest
levels at 30 h PI (33 % of the initial value), and returned to the reference value from 96 h PI to
the end of the experiment. Hepcidin mRNA expression increased at 6 h PI and remained high
at 18 h PI. The iron plasma concentration was an earlier indicator of inflammatory processes
in horses when compared with fibrinogen and might be useful for the early detection of
inflammation in the horse. FCA administration caused the rapid onset of hypoferremia, and
this effect was likely the result of up-regulated hepatic hepcidin gene expression. This study
emphasizes the importance of hepcidin and iron metabolism during inflammation in horses.
Descrição
Palavras-chave
Hepcidina, Adjuvante completo de Freund, Reagente de fase aguda, Inflamação, Metabolismo do ferro, Cavalo, Hepcidin, Freund’s adjuvant, Acute-phase reactants, Inflammation, Iron metabolism, Horses
Citação
OLIVEIRA FILHO, José P.; BADIAL, Peres R.; CUNHA, Paulo Henrique J.; BORDON, Ana Paula; ARAÚJO JÚNIOR, João P.; DIVERS, Thomas J.; WINAND, Nena J.; BORGES, Alexandre S. Freund’s adjuvant-induced inflammation: clinical findings and its effect on hepcidin mRNA expression in horses. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 35, n. 1, p. 51-56, Jan. 2014.