EVZ - Artigos publicados em periódicos

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Suspected glyphosate poisoning in a dog
(2025) Souza, Marcelino Benvindo de; Silva, Rayanne Henrique Santana da; Almeida, Sara Sueli Ferreira de; Leopoldino, Cilho Gabriel Rodrigues; Leopoldino, Ana Gabriela Rodrigues; Passos, Pedro Henrique Miranda; Paula, Luiza Gabriella Ferreira de; Silva, Daniela de Melo e; Martins, Danieli Brolo; Botelho, Ana Flávia Machado
Glyphosate is a widely used pesticide that has been associated with poisoning in both humans and animals. Its toxicity remains a topic of controversy, particularly regarding its carcinogenic potential. This study aimed to investigate a suspected case of glyphosate poisoning in a dog, providing a detailed account of the case history, clinical evolution, laboratory findings, and genotoxic assessment. A seven-month-old dog was referred to the Veterinary Hospital, presenting with mental confusion and ataxia. The pet guardian reported altered consciousness and incoordination twelve days after Roundup® was applied to the farm’s grass. A complete blood count revealed normocytic normochromic anemia, a packed cell volume of 20%, thrombocytopenia (50,000/µL), and severe leukocytosis (447,200/µL), with 95% of the white blood cells consisting of blasts of unidentified origin and 5% segmented neutrophils. Myelogram analysis showed a moderately cellular bone marrow sample with a predominance of primitive cells of undetermined origin (>95%), suggesting a diagnosis of leukemia. To further investigate the potential link between leukemia and glyphosate exposure, whole blood was collected in a heparinized tube for a comet assay. The test indicated moderate DNA damage. Chemotherapy was recommended; however, treatment was not pursued for personal reasons, and the patient died five days after the consultation. A necropsy was not authorized. This report contributes to the understanding of glyphosate poisoning and its potential health consequences, particularly its association with hematologic malignancies in canines.
Experimental assessment of beauvericin toxicity in tadpoles of Dendropsophus minutus (Anura: Hylidae)
(2025) Vicente, Monica Chacon de; Paula, Luiza Gabriella Ferreira de; Freitas, Renata Maria Pereira de; Almeida, Adieli dos Anjos; Lima, Diego da Silva; Gabriel, Rebeca dos Santos; Nogueira, Nathany Geraldino; Batista, Nádya Raquel dos Santos; Barros, Alexandre Có Mangoni; Souza, Marcelino Benvindo de; Bastos, Rogerio Pereira; Brito, Pedro Vale de Azevedo; Silva, Daniela de Melo e; Botelho, Ana Flávia Machado
Beauvericin (BEA) is an emerging mycotoxin frequently detected in food and feed, raising concerns about its toxicological risks for One Health. This study evaluated the acute toxicity of BEA in tadpoles of Dendropsophus minutus, a bioindicator species of environmental toxicity, after a 96-h exposure. Tadpoles were exposed to three concentrations of BEA, with vehicle (DMSO), positive (cyclophosphamide), and negative (water) controls. Outcomes included survival, genotoxicity (comet assay), mutagenicity (micronucleus test), leukocyte profiles, redox status markers, and liver histopathology. Exposure to BEA did not induce genotoxicity; however, it resulted in mutagenic effects, with an increase in reniform nuclei and a decrease in anucleated erythrocytes, and hepatotoxicity characterized by sinusoidal congestion, reduced hepatocyte cytoplasmic volume, and increased connective tissue. Elevated levels of nitric oxide and carbonyl proteins were observed, indicating oxidative stress, while antioxidant enzymes and acetylcholinesterase activity remained unchanged. These findings demonstrate that BEA induces mutagenic, cytotoxic, and hepatotoxic effects in amphibians, highlighting its potential ecological impact and relevance for One Health risk assessment.
Influence of temperature and storage time on the stability of biochemical parameters in broilers
(2025) Cifuentes Ruiz, Katalina; Qualhato, Andriele Ferreira; Albuquerque, Luis Fernando Duarte; Trentin, Thays de Campos; Santos, Ronan Rodrigues dos; Barragán Ruiz, Carmen Elena; Marín Oviedo, Gonzalo; Café, Marcos Barcellos; Botelho, Ana Flávia Machado; Martins, Danieli Brolo
Poultry farming is a strategic sector of Brazilian agribusiness, requiring high health standards to ensure productivity and competitiveness. Biochemical tests are essential for monitoring poultry health, but pre-analytical variables can compromise their results. This study evaluated the influence of storage time and temperature on the stability of 14 serum analytes in COBB 500 broilers, both males and females, aged 35 and 40 days. Samples were analyzed under different conditions: room temperature (27 ± 2°C), refrigeration (6 ± 2°C), freezing (−20 ± 5°C), and ultra-freezing (−80 ± 5°C), over periods of up to 365 days. Analyte stability varied according to the analyte and storage condition. Lactate was the only parameter that remained stable under all tested condition. It is concluded that the preservation of biochemical analytes depends directly on storage conditions, which must be carefully considered to ensure the reliability of results in healthy broilers.
Correlation between the ultrasonographic measurement of intestinal thickening and laboratory parameters in asymptomatic dogs
(2025) Oliveira, Iago Martins; Almeida, Sara Sueli Ferreira de; Sprícigo, Carolle Vieira Muterlle; Silva, Wanessa Patrícia Rodrigues da; Silva, Marco Augusto Machado; Botelho, Ana Flávia Machado; Martins, Danieli Brolo; Borges, Naida Cristina
Background: Intestinal ultrasound is a valuable diagnostic tool for gastrointestinal diseases in dogs. Despite its usefulness, the clinical significance of intestinal thickening in asymptomatic animals remains unclear. Aim: This study aimed to correlate the thickness of the intestinal layers with hematological and biochemical parameters in asymptomatic dogs. Methods: Thirty healthy dogs were assessed and divided into two groups: G1 (n = 15), with no ultrasound alterations, and G2 (n = 15), with different intestinal thicknesses. Pearson’s correlation test was used to assess possible associations between laboratory data and intestinal thickening. Results: The results showed no statistically significant correlation between intestinal thickness and keratometric parameters, leukometry, proteinogram, and serum biochemistry (r ranging from -0.004 to 0.29 in hematology and from 0.008 to 0.2 in biochemistry, with p > 0.05). Conclusion: Therefore, identifying early markers for the diagnosis of enteropathies in asymptomatic dogs was not possible. The lack of associations limits the clinical usefulness of intestinal ultrasound as a predictor of laboratory alterations or as a tool for diagnosing enteropathies in the early stages. Expanding the sample, using additional biomarkers, conducting prospective studies, and correlating ultrasound findings with intestinal histology are recommended. These steps could help explore new clinical and laboratory markers to better understand the clinical significance of gastrointestinal tract thickening in dogs.
Evaluation of Scalibor® deltamethrin collar chronic exposure in dogs
(2025) Rodrigues, Larissa T. P.; Qualhato, Andriele Ferreira; Sprícigo, Carolle Vieira Muterlle; Arantes, Kelly Carolina Assis; Silva, Rayanne Henrique Santana da; Barros, Alexandre Có Mangoni; Vicente, Monica Chacon de; Souza, Murilo R.; Paula, Luiza Gabriella Ferreira de; Campos, Danilo Roman; Botelho, Ana Flávia Machado
Deltamethrin is a pesticide used worldwide with several applications in the control of nuisance arthropods in homes, agriculture, against ectoparasites in dogs and livestock. Recent studies indicate that deltamethrin can induce cardiotoxicity. Therefore, this study aimed to evaluate the toxicity of deltamethrin in dogs through exposure to commercial collars over 8 months. Twenty healthy dogs were selected, 9 males and 11 females, weighing between 10 and 15 kg and aged between 1 and 8 years, with no history of intentional exposure to pyrethroids. Clinical, cardiological, and laboratory evaluations were performed monthly. The results demonstrate that exposure to deltamethrin 4 % via the dermal route, through a commercial collar, was safe for 8 months, with preserved cardiac electrocontractile function, assessed by electrocardiogram and echocardiogram. In addition, tracking of plasma biochemical markers did not indicate signs of cardiac, renal, or hepatic injury. Although deltamethrin 4 % collars demonstrated an acceptable safety profile regarding cardiovascular and laboratory evaluations, the occurrence of dermatological reactions in 25 % of the animals, which is a significant proportion, highlights the need for careful monitoring of dermatological reactions. Future investigations with different concentrations and longer periods are recommended to achieve a more comprehensive understanding of the long-term effects of deltamethrin, improving its safe application in veterinary medicine and promoting animal and human health.
Breaking antibiotic resistance: porphyrin-driven photoinactivation and priming effect on mastitis-related MDR bacteria
(2025) Pires, Luiz Henrique Barbosa; Oliveira, Taise Maria dos Anjos; Desordi, Jaqueline Cristine; Ducas, Eli Silveira Alves; Souza, Lucas Soares; Moraes, Edynara Cruz de; Borsanelli, Ana Carolina; Batista, Alzir Azevedo; Gonçalves, Pablo José; Souza, Guilherme Rocha Lino de
Bovine mastitis is a major infectious disease in dairy herds worldwide, with increasing prevalence of multidrug-resistant (MDR) pathogens affecting the efficacy of conventional antibiotic therapies. In this study, we explore antimicrobial photodynamic inactivation (PDI) as an innovative and sustainable strategy for controlling mastitis-associated bacteria. The photoinactivation potential was evaluated for four photosensitizers (PS) - palladium(II)/diphosphine-coordinated meso-tetrapyridyl porphyrins - Porf@DPPE, Porf@DPPP, Porf@DPPB, and Porf@DPPF - against six MDR bacterial strains isolated from bovine mastitis cases. Porf@DPPE, Porf@DPPP, and Porf@DPPB showed high triplet (ΦT > 0.72) and singlet oxygen (ΦΔ > 0.62) quantum yields, while Porf@DPPF, despite lower values (ΦT = 0.46; ΦΔ = 0.42), exhibited higher lipophilicity. All compounds induced significant photoinactivation, with Porf@DPPE achieving the lowest effective concentration (6.25 μM) and the broadest antimicrobial spectrum. Gram-negative isolates, such as Escherichia coli and Pseudomonas aeruginosa, exhibited reduced susceptibility, requiring higher PS concentrations. DNA integrity assays indicated a potential PS mechanism of action on pathogens. Furthermore, combined treatment with Porf@DPPE or Porf@DPPF and sulfonamides restored antibiotic susceptibility in MDR E. coli, highlighting a synergistic interaction. These findings underscore the therapeutic potential of metalloporphyrin-based photosensitizers for mastitis control and support the integration of PDI with conventional antibiotics as a feasible approach to overcome multidrug-resistant bacteria in veterinary settings.
Epidemiological survey on the oral health of cattle slaughtered in Goiás, Brazil
(2025) Caetano, Geovanna Domingues da Cunha; Martins, Andressa da Silva; Cardoso, Maria Vitoria Soares; Paula, Warley Vieira de Freitas; Saraiva, Júlia Rebecca; Borges, Naida Cristina; Schweitzer, Christiane Marie; Jardim Júnior, Elerson Gaetti; Dutra, Iveraldo dos Santos; Borsanelli, Ana Carolina
Background Good oral health is essential for the performance and welfare of cattle, as it directly affects their feed intake and feed efficiency. However, little is currently known about the epidemiology of dental disease in cattle. Methods This study investigated the prevalence of lesions in the dental arcades of 1006 slaughtered cattle. The periodontal evaluation was performed by the same person, using a periodontal probe and flashlight as the heads passed down the slaughter line, and the results were recorded on an odontogram. Results Of the 1006 slaughtered animals evaluated, 115 (11.4%) were male and 891 (88.6%) were female; 735 (73.1%) were beef cattle and 271 (26.9%) were dairy cattle. Gingival recession was more frequent in incisors, while periodontal pockets were observed mainly in incisors and molars. Dairy cattle showed a higher prevalence of gingival recession and periodontal pockets than beef cattle. In beef cattle, an association was observed between age and gingival recession, while in dairy cattle, age was linked to tooth wear. No significant differences were observed between males and females. Limitations As the examinations were carried out postmortem, it was not possible to evaluate the prevalence of gingivitis. Conclusion The findings of this study contribute to our understanding of the complex relationship between sex, breed type, age and oral health in cattle, highlighting the need for further research to clarify these associations and develop targeted management strategies to promote animal health and welfare.
Anti-Brucella abortus antibodies in equids in the state of Goiás, Brazil
(2025) Martins, Andressa da Silva; Romanowski, Tatiana Nunes de Azevedo; Morais, Janaina Silva Campos de; Vieira, Rafael Costa; Silva, Tamires Ataides; Borsanelli, Ana Carolina
Brucellosis is a zoonosis caused by bacteria of Brucella genus, which affects domestic and wild animals and humans. In horses, the infection can be caused by Brucella canis, Brucella suis or Brucella abortus, through the ingestion of contaminated food, contact of bacteria with skin wounds or through the respiratory tract, manifesting with signs such as fistulous withers and reduced performance. The present study aimed to evaluate the seroprevalence of Brucella abortus infection in equids in the state of Goiás. Sampling was carried out on 299 farms across 132 municipalities, with a total of 897 equids, including horses, donkeys and mules. Among the animals tested, 111 reacted to the rose bengal plate agglutination test (RBPAT), with 40 confirmed as reactive by the 2-Mercaptoethanol (2-ME) test, resulting in a seroprevalence of 4.46 %. Among the reactive animals, 87.5 % were horses, 12.5 % were mules and no donkeys were reactive in this study. The distribution of seropositive animals was dispersed throughout the state of Goiás, suggesting that brucellosis is not restricted to a specific region. Furthermore, statistical analysis revealed no significant association between the variables age, species, sex, purpose, and stratum and positivity in the 2-ME test. Of the 34 herds with reactive animals, 82.35 % had only one reactive animal, which suggests that equines are becoming infected through contact with infected cattle. Thus, the results reinforce the need for integrated disease management for both bovine and equine brucellosis, due to its zoonotic potential and the possibility of cross-infection with other species.
Clostridium septicum-associated myonecrosis in a dog: case report
(2025) Amorim, Isabele Maurer; Oliveira, Luiz Gustavo Schneider de; Boabaid, Fabiana Marques; Martins, Andressa da Silva; Gomes, Leidiane de Souza; Paixão, Fernanda Martins da; Falcão, Ingrid Carneiro de Oliveira; Martins, Danieli Brolo; Carvalho, Rosângela de Oliveira Alves; Vasconcelos, Aline Maria; Borsanelli, Ana Carolina
Necrotizing soft tissue diseases are severe, rapidly progressive infections characterized by tissue destruction and toxemia, often leading to death. Although common in livestock, myonecrosis caused by toxinogenic clostridial species is rarely reported in dogs. This study reports a case of myonecrosis in a male mongrel dog attacked by stray dogs, which subsequently developed a Clostridium septicum infection. The dog, prostrate and in contact with the ground, was brought for treatment two days post-attack, presenting multiple bite wounds, particularly on the pelvic limbs and scrotum, and a scrotal abscess. The condition progressed to toxemic shock, with extensive necrosis and emphysema in subcutaneous tissue and muscles. Due to clinical deterioration, euthanasia was performed. Postmortem findings revealed widespread edema, hemorrhage, and emphysema in the affected skeletal muscle and subcutaneous tissue of the pelvic limbs and scrotum, alongside moderate multifocal purulent exudate in the muscle fascia. Histological analysis identified myonecrosis with perimysium and subcutaneous tissue expansion due to hemorrhage, proteinaceous material, and emphysema. Suppurative infiltrates containing gram-positive, rod-shaped bacteria were present within the fascia. PCR analysis confirmed C. septicum in samples of affected skeletal muscle. This case highlights the importance of early diagnosis and management of traumatic infections in dogs, identifying bite wounds as entry points for clostridial pathogens and soil or saliva as spore sources. It contributes to understanding the pathogenesis and management of these infections in dogs, emphasizing their poor prognosis and the urgency of addressing clostridial myonecrosis.
In vitro evaluation of the bactericidal effect of ozonated water on multidrug-resistant shigatoxigenic Escherichia coli: a pilot study
(2025) Moraes, Isabela Silva; Queiroz, Pedro Henrique Pereira de; Carvalho, Stephani Félix; Rocha, Maria Cristina Veríssimo; Silva, Camila dos Reis e; Nascente, Eduardo de Paula; Borsanelli, Ana Carolina; Queiroz, Paulo José Bastos; Silva, Luiz Antônio Franco da
The bactericidal properties of ozone have been studied in the treatment of various diseases, including the prevention and treatment of mastitis. With the increase in cases of bacterial resistance and the risk to public health due to residues in milk, the search for new treatments is essential. Therefore, in this pilot study, we aimed to assess the antimicrobial effect of ozonated water against multidrug-resistant Shigatoxigenic Escherichia coli, a causative agent of bovine mastitis. For in vitro tests, three groups were evaluated at three contact times (30 seconds, 1 minute and 5 minutes). The tested groups included control (0.85% saline solution), 0.2% chlorhexidine gluconate (CHG) and ozonated water (OZO = 3.3 ± 0.4 µg/mL). After conducting the tests, the groups were assessed for colony-forming unit (CFU) counts to determine the effectiveness of each treatment. Ozonated water (OZO) demonstrated bactericidal activity statistically equivalent to that of chlorhexidine gluconate (CHG) against a multidrug-resistant Shigatoxigenic Escherichia coli strain. Therefore, it could serve as a potential alternative to antiseptics commonly used in pre-milking procedures for dairy cows.
Feline botulism: two decades and two reports
(2025) Braga, Ísis Assis; Urzedo, Nicoly Ferreira de; Maia, Guilherme Oliveira; Gomes, Ana Paula Carvalho; Duarte, Raiany Borges; Ribeiro, Bruna Samara Alves; Martins, Andressa da Silva; Silva, Tamires Ataides; Pires, Luiz Henrique Barbosa; Ferreira, Stéfanne Rodrigues Rezende; Borsanelli, Ana Carolina
This study describes an outbreak of type C botulism in domestic cats in central region of Brazil, two decades after the only previously reported natural case in the specie. Between August and October 2024, 21 cats presented with flaccid tetraparesis and were clinically evaluated. The course of the clinical signs varied from acute to subacute, with signs including hypotonia of all limbs, bladder and intestines, hypophonia, and flaccid paralysis of masticatory muscles. Eight cats died due to cardiorespiratory arrest. Necropsy and histopathological analyses revealed no significant macroscopic or microscopic lesions. Botulinum toxin type C was detected in five cats through standard mouse bioassay: in feces (n = 3), liver (n = 1), and intestinal contents (n = 1). Although the toxin was not found in the remaining animals, botulism was not excluded based on clinical and epidemiological findings, absence of spinal or other neurological lesions, and the exclusion of other infectious agents. All cats tested negative for FeLV, Coronavirus, Toxoplasma gondii, and Neospora caninum, and were also screened for intestinal parasites. Despite the strong epidemiological evidence, including common exposure to commercial diets, hunting behavior, and outdoor access, the source of intoxication was not identified. This study highlights a rare and severe outbreak of feline botulism and reinforces the importance of considering this diagnosis in similar clinical scenarios.
Spatial distribution of equid exposure to Rickettsia spp. in Goiás state, midwestern Brazil
(2025) Pádua, Gracielle Teles; Tavares, Mariana Avelar; Lima, Nicolas Jalowitzki de; Paula, Warley Vieira de Freitas; Santos, Gabriel Cândido dos; Neves, Lucianne Cardoso; Bittencourt, Raphaela Bueno Mendes; Paludo, Raquel Loren dos Reis; Cardoso, Ennya Rafaella Neves; Silva, Bianca Barbara Fonseca da; Borsanelli, Ana Carolina
This study sought to investigate the presence of anti-Rickettsia spp. antibodies in georeferenced serum samples from equids across all regions of the state of Goiás, while also presenting variables that indicate risk factors for the circulation of rickettsiae, and evaluating the presence of rickettsial DNA in ticks collected from equids and the surrounding environment in the municipalities of Uruaçu and Porangatu, located in the northern region of the state. A total of 1156 equid serum samples provided by the Goiás Agricultural Defense Agency (Agrodefesa) were analyzed for antibodies against 4 Rickettsia antigens. Additionally, 39 blood samples were collected from equids during a 3-day field expedition in January 2024, alongside 938 ticks collected from both animals and the environment. In total, 297 adult ticks were randomly selected for molecular analysis and tested by qPCR targeting the rickettsial gltA gene. Positive samples were further analyzed using cPCR to target the ompA and gltA genes. Results revealed that 9.6% (111/1156) of the serum samples were seroreactive to at least one Rickettsia antigen. Among these, 36% had antibodies against Rickettsia rickettsii, 18.9% against Rickettsia parkeri, 69.4% against Rickettsia amblyommatis, and 14.4% against Rickettsia bellii. Furthermore, the possible antigen responsible for a homologous reaction was found in 3.6% of equids for R. rickettsii, and in the same proportion for R. bellii, while 23.4% of animals showed antibodies for R. amblyommatis, and none exhibited a homologous reaction to R. parkeri. Meanwhile, 28.2% of the serum samples collected during the field expedition had antibodies against R. amblyommatis, with 72.7% identifying R. amblyommatis as the antigen involved in the homologous reaction. In the logistic regression analysis, the variables of education level, altitude below 500 m, and presence of female mules demonstrated a positive effect on seropositivity. Ticks from animals were identified as Amblyomma cajennense sensu lato, Amblyomma sculptum, Dermacentor nitens, and Rhipicephalus microplus, while environmental ticks were identified as A. cajennense s.l., A. sculptum, and Amblyomma nodosum. In the qPCR assays, two specimens of A. cajennense s.l., one of A. sculptum, and one of D. nitens amplified a fragment of the gltA gene. Of these, one A. cajennense s.l. specimen amplified a fragment of both the ompA and gltA genes, and one A. sculptum specimen amplified a fragment of the gltA gene through conventional PCR. Sequencing confirmed the detection of R. amblyommatis. These findings highlight the presence of anti-Rickettsia spp. antibodies in equid serum samples from all regions of the state of Goiás, emphasizing the important role of equids as sentinels for Rickettsia spp. To our knowledge, this study represents the largest effort to detect anti-Rickettsia spp. antibodies in equid serum samples in Brazil. Additionally, it is the first nationwide investigation of its kind conducted in collaboration with the Agricultural Defense Agency (Agrodefesa), serving as a significant example of the One Health approach.
Botulism outbreak in cattle due to ingestion of accidental deteriorated feed
(2025) Paz, Milena Carolina; Moraes, José Tiago Roza de; Molossi, Franciéli Adriane; Martins, Andressa da Silva; Silva, Tamires Ataides; Marques, Luciane Oliveira; Kowalski, Ananda Paula; Borsanelli, Ana Carolina; Panziera, Welden; Driemeier, David
Botulism is a condition caused by the ingestion of pre-formed toxins produced by the bacterium Clostridium botulinum. This study described an outbreak of type D botulism with a high mortality rate (67%) in a cattle herd, caused by the consumption of fermented silage. The affected cattle presented with a hyperacute to acute clinical course with progressive flaccid paralysis. Five animals underwent necropsy with no significant macroscopic and microscopic alterations. A most probable source of contamination was deemed to be an inadequately stored silage; although, the toxin was not detected in the feed. The diagnosis of botulism was based on both clinical and epidemiological findings and the detection of botulinum toxin in the intestinal contents of two of the affected cattle. The present study emphasized the high number of fatally affected cattle, an unusual source of botulinum toxin and the importance of proper management of animal feed supply.
Simultaneous occurrence of type C botulism in poultry and dogs
(2025) Martins, Andressa da Silva; Alves, Lisandra Rodrigues; Silva, Tamires Ataides; Santos, Ronald M.; Almeida, Ana Maria de Souza; Saraiva, Júlia Rebecca; Dutra, Iveraldo dos Santos; Borsanelli, Ana Carolina
Botulism is a serious disease caused by neurotoxins produced by Clostridium botulinum, an anaerobic spore-forming bacterium commonly found in the environment and the intestinal tract of animals. The disease occurs through the ingestion of food or water contaminated with preformed toxins, leading to progressive flaccid paralysis across various susceptible species. This study reports the clinical, epidemiological, and laboratory aspects of the simultaneous occurrence of type C botulism in domestic birds (chickens and ducks) and dogs on a farm in Rio Pomba, Minas Gerais, Brazil. The farm housed 47 chickens, two ducks, and seven dogs. Over approximately 15 days, 40 chickens (85.1%) and one duck (50%) fell ill and died. Among the seven dogs, five (71.4%) exhibited clinical signs, and two (28.6%) ultimately died. Diagnosis of botulism was confirmed through bioassay technique and neutralization using homologous antitoxin in mice, which identified botulinum toxin type C in serum samples from two chickens with clinical signs, in samples from two necropsied chickens and in one water sample. The most probable transmission route was likely the remains of a decomposing bovine carcass, and the water that had accumulated around it, to which the chickens, ducks and dogs had access, highlighting the importance of proper carcass disposal to prevent disease outbreaks.
Microbiological quality of sugarcane juice sold in Goiânia/GO and risks to public health
(Universidade Federal de Goiás, 2025-01-23) Messias, Aline Cristine Magalhães da Costa; Paixão, Eric Vinicius Resende; Marques, Leandro Túlio Santana; Rosa, Leandro Marcos da Silva; Queiroz, Pedro Henrique Pereira de; Silva, Carla Afonso da
Sugarcane juice has a pleasant flavor, is easy to process, and is well-accepted by the general population. However, microbiological quality is not always assured, which can result in risks for consumption. The objective of this study was to evaluate the microbiological quality of sugarcane juice obtained from commercial establishments in the city of Goiânia. Sugarcane juice samples were collected from 21 locations between February and July 2018. The analysis was performed using the multiple tube technique, and quantification by the most probable number (MPN) of microorganisms present in each sample. The microbiological analysis investigated total and thermotolerant coliforms, in addition to Salmonella, as recommended by current legislation. Total coliforms were found in 100% of the samples, with a count >1600 MPN/mL in 57.1%. For thermotolerant coliforms, 100% of the samples were positive, with 33.3% being above the legal limit and 19.1% being positive for Escherichia coli. Among the 21 samples evaluated, one was positive for Salmonella ser. Typhimurium. The quantity and quality of microorganisms isolated in the sugarcane juice samples sold in the city of Goiânia indicate the need to improve the hygienic and sanitary quality of the establishments. There is an urgent need to establish training courses for traders in this sector to provide guidance on the correct way to process, store, and transport food and raw materials, which will guarantee the quality of the food and protect the health of the consumers.
Síndrome da malformação occipital caudal
(2017) Torres, Bruno Benetti Giunta
Trauma crânio-encefálico: parte 1
(2017) Torres, Bruno Benetti Giunta
Epilepsia em cães e gatos
(2017) Torres, Bruno Benetti Giunta
Síndrome da hiperestesia felina
(2017) Torres, Bruno Benetti Giunta
Association of riluzole and dantrolene improves significant recovery after acute spinal cord injury in rats
(2018) Martins, Bernardo De Caro; Torres, Bruno Benetti Giunta; Oliveira, Karen Maciel de; Lavor, Mario Sergio; Osório, Carla Maria; Fukushima, Fabiola Bono; Rosado, Isabel Rodrigues; Melo, Eliane Gonçalves de
Background Context Damage to the spinal cord can result in irreversible impairment or complete loss of motor, sensory, and autonomic functions. Riluzole and dantrolene have been shown to provide neuroprotection by reducing neuronal apoptosis after brain and spinal cord injury (SCI) in several animal models of neurologic disorders. As these drugs protect the injured spinal cord through different mechanisms, we investigated the cumulative effects of riluzole and dantrolene. Purpose This study aimed to investigate the neuroprotective efficacy of the combined administration of riluzole and dantrolene in experimental thoracic SCI. Study Design Twenty-nine Wistar rats were laminectomized at T12 and divided in five groups. Rats in GI (n=6) underwent laminectomy alone and were treated with placebo. Rats in GII (n=6) underwent laminectomy followed by SCI and were treated with placebo. Rats in GIII (n=5) underwent laminectomy followed by SCI and were treated with riluzole and placebo 15 minutes and 1 hour after laminectomy, respectively. Rats in GIV (n=6) underwent laminectomy followed by SCI and were treated with placebo and dantrolene 15 minutes and 1 hour after laminectomy, respectively. Rats in GV (n=6) underwent laminectomy followed by SCI and were treated with riluzole and dantrolene 15 minutes and 1 hour after laminectomy, respectively. A compressive trauma was performed to induce SCI. Methods Behavioral testing of hind limb function was performed using the Basso Beattie Bresnahan locomotor rating scale, which revealed significant recovery in the group treated with the association of riluzole and dantrolene compared with other groups. After euthanasia, the spinal cord was evaluated using light microscopy and immunochemistry with anti-NeuN and transferase dUTP nick-end-labeling (TUNEL) staining. Results Animals treated with the association of riluzole and dantrolene showed a larger number of NeuN-positive neurons adjacent to the epicenter of injury (p≤.05). Furthermore, the TUNEL staining was similar between animals treated with riluzole and dantrolene and those that did not receive spinal cord trauma (p>.05). Conclusions These results showed that riluzole and dantrolene have a synergistic effect in neuroprotection after traumatic SCI by decreasing apoptotic cell death.

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