Evaluation of the antibacterial activity of Crotalus durissus terrificus crude venom
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Data
2018-10-20
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Editor
Universidade Federal de Goiás
Resumo
Snake venoms are recognized as a promising source of pharmacologically active substances and are
potentially useful for the development of new antimicrobial drugs. This study aimed to investigate
the antimicrobial activity of the venom from the rattlesnake Crotalus durissus terrificus against
several bacteria. Antibacterial activity was determined by using the plate microdilution method and
the activity on the bacterial envelope structure was screened by using the crystal violet assay. The
proteins in crude venom were separated by electrophoresis and characterized regarding their
proteolytic activity. C. d. terrificus venom exhibited antimicrobial action against gram-positive and
gram-negative bacteria. MIC values were defined for Pseudomonas aeruginosa ATCC 27853 (62.5
μg/mL), Staphylococcus aureus ATCC 25923 (125 μg/mL), and Micrococcus luteus ATCC 9341
(≤500 μg/mL). For Salmonella enterica serovar typhimurium ATCC 14028 and Corynebacterium
glutamicum ATCC 13032, the decrease in bacterial growth was not detected visually, but was
statistically significant. The crystal violet assay demonstrated that the crude venom increased
bacterial cell permeability and the secreted protein profile agreed with previous reports. The results
suggest that the proteins with lytic activity against bacteria in C. d. terrificus venom deserve further
characterization as they may offer reinforcements to the weak therapeutic arsenal used to fight
microbial multidrug resistance.
Descrição
Palavras-chave
Rattlesnake venom, Antimicrobial action, Cellular envelope, Proteolytic activity, Veneno de cascavel, Ação antimicrobiana, Envelope celular, Atividade proteolítica
Citação
DIETZ, Jefferson do Carmo; ALMEIDA, Daniela Andrade de; CINTRA, Lorena Cardoso; OLIVEIRA, Bruno Francesco Rodrigues de; MAGALHÃES, Marta Regina; JESUÍNO,Rosália Santos Amorim. Evaluation of the antibacterial activity of Crotalus durissus terrificus crude venom. Ciência Animal Brasileira, Goiânia, v. 19, e-51322, 2018. Disponível em: https://revistas.ufg.br/vet/article/view/e-51322. Acesso em: 6 out. 2022.