Extrato etanólico de cascas recicladas de pequi (Caryocar brasiliense, Camb.) induz apoptose em células de osteossarcoma canino

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Data

2022-03-15

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Universidade Federal de Goiás

Resumo

Osteosarcoma is a mesenchymal cancer associated with high mortality in dogs and in humans. The biodiversity-rich Cerrado, the predominant biome in the Midwest region of Brazil, is native to locally appreciated fruits such as pequi (Caryocar brasiliense, Camb). Although this plant has been frequently used in folk medicine, the pharmacological properties of pequi fruit shells have not been fully evaluated. Thus, this study aimed to determine the cytotoxic activity of ethanol extract of recycled pequi shells on canine osteosarcoma cells in vitro. Cells were cultured and treated with final extract concentrations of 0, 0.029 µg/µL, 0.29 µg/µL, and 2.91 µg/µL for 24, 48, or 72 hours. Cell viability assay using trypan blue exclusion method and tetrazolium reduction method, cell survival assay, and double labeling with annexin V and propidium iodide were performed in the treated osteosarcoma cells. These allowed the determination of IC50, survival fraction, and type of cell death, respectively. Pequi shell ethanol extract at a concentration of 2.91 µg/µL showed the greatest inhibition of osteosarcoma cell growth in vitro, resulting in a 71.80% decrease in growth compared to the control. The mean IC50 was 155.2 μg/mL at 72 hours. The calculated survival fractions showed that cell growth at 72 hours was 3.33% lower in cells treated with 2.91 µg/µL extract. Results from the double labeling experiment suggest that apoptosis was the predominant type of cell death in cells treated with 2.91 µg/µL extract. These results demonstrate that ethanol extract of recycled pequi shells promotes apoptosis in canine osteosarcoma cells.

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Palavras-chave

Antineoplásico, Cerrado, D-17, Osteossarcoma canino, Pequi, Sustentabilidade, Taninos, Antineoplastic, Canine osteosarcoma, Cerrado biome, Sustainability, tannins

Citação

BRAGA, Karla Márcia da Silva; CRUZ, Vanessa de Sousa; ARNHOLD, Emmanuel; ARAÚJO, Eugênio Gonçalves de. Extrato etanólico de cascas recicladas de pequi (Caryocar brasiliense, Camb.) induz apoptose em células de osteossarcoma canino. Ciência Animal Brasileira, Goiânia, v. 23, n. 1, e71198, 2022. Disponível em: https://www.revistas.ufg.br/vet/article/view/71198.