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dc.creatorGalvão, Lúcia Maria da Cunha-
dc.creatorChiari, Egler-
dc.creatorMacedo, Andréa Mara-
dc.creatorOstermayer, Alejandro Luquetti-
dc.creatorSilva, Simonne Almeida e-
dc.creatorAndrade, Ana Lúcia Sampaio Sgambatti de-
dc.identifier.citationGALVÃO, Lúcia M. C.; CHIARI, Egler; MACEDO, Andréa M.; LUQUETTI, Alejandro O.; SILVA, Simonne A.; ANDRADE, Ana Lúcia S. S. PCR assay for monitoring Trypanosoma cruzi parasitemia in childhood after specific chemotherapy. Journal of Clinical Microbiology, Washington, v. 41, n. 11, p. 5066-5070, Nov. 2003.pt_BR
dc.identifier.issne- 1098-660X-
dc.description.abstractAssessment of cure of Trypanosoma cruzi infection by antibody seroconversion usually involves several years of follow-up. Parasitological negativity is useless for cure assessment, since even untreated patients mostly show negative results; conversely, positive tests are of great value because they indicate treatment failure. Here, PCR was used to assess the rate of specific chemotherapy failure in a well-characterized Brazilian cohort of T. cruzi-seropositive children, who were enrolled in a field trial of benznidazole (Bz) efficacy. Paired blood samples from 111 children were taken at baseline and 36 months after treatment with either Bz (n 58) or a placebo (n 53). DNA extraction and PCR amplification were carried out as previously described, and hybridization was performed with all PCR products. At the end of follow-up, PCR was positive for 39.6% of the patients in the Bz group versus 64.2% in the placebo group (P 0.01). Untreated patients had a 1.6-fold-higher chance of remaining positive by PCR than those in the Bz group (P < 0.05). We conclude that PCR is a useful tool for revealing therapeutic failure of T. cruzi infection on a short-term basis.pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectSpecific chemotherapypt_BR
dc.subjectDiagnosis of Chagas diseasept_BR
dc.subjectTrypanosoma cruzi infectionpt_BR
dc.titlePCR assay for monitoring Trypanosoma cruzi parasitemia in childhood after specific chemotherapypt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
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