Desenvolvimento de método analítico de cromatografia líquida de alta eficiencia (CLAE) para quantificação de lupeol em nanocápsulas poliméricas
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Data
2014
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Resumo
A simple, precise and accurate high-performance liquid
chromatographic method has been developed for the
determination of lupeol in polymeric nanocapsules.
Chromatographic separation was performed on
a Varian C8 column (250 mm x 4.6 mm x 5 mm)
maintained at 35°C, with a mobile phase composed of
acetonitrile and methanol (95:5 v/v) acidified with 0.1%
acetic acid, flowing at 1.2 mL/min, with an injected
sample volume of 20 μL, UV detection at 210 nm and
a run time of 6.2 min. The proposed method was linear
over the concentration range 10-250 μg/mL, with R2
= 0.9996. Analyses of accuracy and precision showed
low values of relative standard deviation (<4.2%). The
methodology was specific, linear, accurate, precise and
robust and proved to be adequate for the quantitative
analysis of lupeol in polymeric nanocapsules.
Descrição
Palavras-chave
Lupeol, Nanocápsulas, CLAE, Lupeol, HPLC, Nanocapsules
Citação
NAVES, Letícia Nasser et al. Desenvolvimento de método analítico de cromatografia líquida de alta eficiencia (CLAE) para quantificação de lupeol em nanocápsulas poliméricas. Revista de Ciências Farmacêuticas Básica e Aplicada, Araraquara, v. 35, n. 3, p. 1473-478, 2014.