Synadenium umbellatum Pax. promotes cell cycle arrest and induces apoptosis in K-562 leukemia cells
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Data
2012-09
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Resumo
Chronic myeloid leukemia (CML) is a clonal myeloproliferative disease that shows apoptosis resistance.
The introduction of imatinib mesylate has revolutionized the treatment of CML, but imatinib resistance
may develop at any time and inevitably leads to disease progression. Synadenium umbellatum Pax. belongs
to the Euphorbiaceae family and is popularly used in Brazil for the treatment of cancer. The cytotoxicity
of Euphorbiaceae is associated with the ability of these plants and their bioactive compounds to induce
apoptotic tumor cell death. Therefore, we aimed to investigate the cytotoxicity and the mechanisms of
death induced by S. umbellatum extract in leukemic cells. S. umbellatum cytotoxicity was evaluated by
trypan blue exclusion assay and flow cytometric analysis of the cell cycle; the mechanisms involved in
K-562 cell death were investigated by light microscopy and flow cytometry. The results demonstrate that
S. umbellatum is cytotoxic to leukemic cells in a concentration-dependent manner. Morphological analysis
revealed that S. umbellatum treatment induced K-562 cell death by an apoptotic pathway. Furthermore,
data indicate ROS overproduction, alterations in mitochondrial membrane potential, phosphatidylserine
externalization and activation of caspase 9. Taken together, the results demonstrate that S. umbellatum
extract arrested the cell cycle and triggered apoptosis at several levels in K-562 cells.
Descrição
Palavras-chave
Synadenium umbellatum/pharmacognosy, K-562 cells/cycle arrest, Synadenium umbellatum/cytotoxicity/evaluation, K-562 cells/apoptosis, Synadenium umbellatum/farmacognosia, Célula K-562/retenção do ciclo, Synadenium umbellatum/citotoxicidade/avaliação, Célula K-562/apoptose, Synadenium umbellatum/farmacognosia, Synadenium umbellatum/citotoxicidade/avaliação, Célula K-562/apoptose, Célula K-562/retenção do ciclo
Citação
MOTA, Mariana Flavia da; BENFICA, Polyana Lopes; VALADARES, Marize Campos. Synadenium umbellatum Pax. promotes cell cycle arrest and induces apoptosis in K-562 leukemia cells. Brazilian Journal of Pharmaceutical Sciences, São Paulo, v. 48, n. 3, p. 10-20, July/Sept. 2012.