Investigação de potencial citotóxico e mecanismos de morte celular de e-líquidos

Abstract

The cigarette is a product of tobacco since 2007 overwhelmingly reaching more young people. The refill this product is e-juice, your composition is propylene glycol and or glycerol, nicotine, flavoring, that through of battery coupled produces aerosols that are inhaled. As WHO, the ANVISA, not consent with the sale and import of that product due to the lack of studies that prove safety for users. The aim of this study was evaluated the product e-liquid (commercial product) of two flavors, two brands and different concentrations of nicotine, propylene glycol 70 and glycerol 30 and flavor of vanilla, which in the literature presented with significant toxicity. Innovative method was used with exposure through the Vitrocell® Cloud 12 system in the epithelial alveolar model 3D. For the evaluation of cell viability used the MTT assay. The e-liquid of menthol with nicotine concentration 0 mg, 3 mg, 5 mg the IC50 was: 2,482%, 2,046% e 0,55% respectively. While the e-líquid of vanilla with nicotine concentration 0 mg, 3 mg, the IC50 was greater than that tested and 0,4160% respectively and the flavor of vanilla was 5 mg/ mL. The concentration 3,54 mg/mL of flavor vanilla was tested on epithelial alveolar model 3D and reduced the viability in 30%. The histological changes observed by coloring HE, showed a tissue inconstancy, more number greater number of cell layers and peeling of the outermost for the tissue treated with flavor of vanilla in concentration the 3,54 mg/mL. The results founded allow the amplification of the view on the commercial products and its components, however it is necessary to standardize the product.