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Estratégias para melhora da biodisponibilidade oral da insulina: uma descrição dos desafios e das abordagens descritas na literatura
(Universidade Federal de Goiás, 2023-08-09) Silva, Marcela Amorim da; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Pascoa, Henrique; Lemes, Érick de Oliveira
Diabetes Mellitus is a metabolism syndrome characterized by a permanent increase in plasma glucose. Conventional treatment, in addition to being painful and uncomfortable, generates psychological stress due to subcutaneous perforation, which may cause side effects of hyperinsulinemia, weight gain and risks of hypoglycemia. About 45-60% of patients abandon conventional treatment due to needle phobia, and oral insulin can minimize these statistics. The main challenges of oral insulin administration are maintaining the integrity of the peptide throughout the gastrointestinal tract, effecting absorption and increasing sufficient bioavailability for its therapeutic effect. Biopharmaceutical strategies can be employed, among which are: delivery systems, enzyme inhibitors, absorption enhancers, mucoadhesive systems and chemical modifications. Some of the strategies demonstrated a significant increase in bioavailability, as well as in structure protection and increased stability, resulting in promising approaches.
Estudo e compreensão do papel da microbiota cutânea como estratégia para o delineamento de cosméticos antienvelhecimento cutâneo
(Universidade Federal de Goiás, 2023-08-09) Pereira, Dyovanna Pazini; Silva, Shirley Souza; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Silva, Andreia Luiza Pereira; Maciel, Iara Mendes
The skin is the largest organ of the human body and plays a vital role in protecting and regulating the organism. It acts as a physical barrier that shields against injuries and external agents such as bacteria, viruses, pollutants, and solar radiation. The skin also assists in regulating body temperature by acting as thermal insulation and aiding in heat dissipation through perspiration. Additionally, it serves to harbor resident microorganisms with important biological functions, known as the microbiome. Hence, the study of the microbiome, referred to as microbiomics, is on the rise. Over the years, the skin undergoes chemical and physical processes that lead to cellular breakdown, resulting in wrinkles and skin aging. The study of omics sciences is essential for developing personalized strategies to maintain or restore the functions of aging skin. The pharmaceutical professional plays a significant role in this field through research and pharmaceutical care. The objective of this work is to discuss and present the existing relationship between skin aging and the microbiome. We will explore how the skin's microbiota can contribute to the development of strategies, such as the formulation of dermocosmetic products, aimed at mitigating or reversing the damages caused by skin aging. Furthermore, the role played by the pharmaceutical professional in this context will be discussed.
Atividade leishmanicida de três derivados de chalcona e sua associação com a rigidez da membrana avaliada pela espectroscopia de RPE
(Universidade Federal de Goiás, 2023-08-11) Borges, Ellyêssa do Nascimento; Alonso, Antonio; Alonso, Antonio; Menegatti, Ricardo; Silva, Kleber Santiago Freitas e
Leishmaniasis is a neglected tropical disease that affects the world's poorest populations. Currently, therapeutic options have high toxicity, with prolonged therapeutic regimens. Still, the emergence of resistance highlights the need to seek new therapeutic agents. Chalcones are precursors of flavonoid biosynthesis with clinical potential for the treatment of leishmaniasis. Novel chalcone-like compounds, LQFM332 (4), LQFM333 (5), and LQFM341 (6), were synthesized and their activity against Leishmania (L.) amazonensis was evaluated using electron paramagnetic resonance (EPR) spectroscopy and assays of cell viability. Compound 4 was synthesized from the molecular hybridization of vanillin (9) and butylated hydroxytoluene (BHT), forming a chalcone scaffold. BHT was replaced by another apocynin to give compound 5, while for compound 6 the Michael acceptor double bond from compound 4 was reduced. For cell viability assays, the IC 50 values for compounds 4, 5, and 6 against the Leishmania parasite were 7, 20, and 16 µM for the promastigote form, and 19, 11, and 1 µM for the amastigote form inside infected macrophages, respectively. EPR spectroscopy of a lipid spin label incorporated into the parasite plasma membrane demonstrated that treatment with these compounds in culture medium causes membrane rigidity in a concentration-dependent manner, owing to oxidative processes. From the IC 50 values with different cell concentrations, the membrane-water partition coefficient (K M/W ) of the compounds could be estimated, as well as their concentrations in the membrane (c m50 ) and in the aqueous culture medium (c w50 ) that reduce antiproliferative activity by 50%. Values for these biophysical parameters were similar to those reported for the antileishmanial drug, miltefosine. K M/W values indicated that the compounds have high affinity for the parasite membrane, with the compound containing the BHT group having the highest affinity. Compound 5, which contained a two 4-hydroxy-3-methoxyphenyl scaffold, showed the highest values of cw50 and cm50 . Compound 6 showed that the reduction of the double bond decreased the membrane affinity and the c w50 value, but maintained the c m50 , demonstrating the same activity as compound 4 for assays with higher concentration of cells. As cytotoxic activities were modulated by effects on the cell membrane, our results suggest that the primary action of the compounds is on the parasite membrane.
Estratégias biotecnológicas para melhoria das características sensoriais e nutricionais dos alimentos
(Universidade Federal de Goiás, 2023-08-04) Silva Júnior, Rodrigo Soares da; Silva, Luís Antônio Dantas; Moraes, Mariana Cristina de; Silva, Aline Gomes de Moura e; Silva, Luís Antônio Dantas
Food biotechnology is a research area that develops improvement activities, as a strategy to increase the stability of the microbiological, nutritional and sensory aspects of food products, which includes the application of various biotechnological methods that aim to avoid changes, whether of enzymatic, physical, microbial or chemical origin, from the origin to the manufacture of the food. These technologies employed, benefit food in nutritional and sensory aspects. This study aims to understand and describe how different strategies can be used effectively and accurately to improve the sensory characteristics of foods, in order to promote a new perspective on it. This is a literature review, focusing on the period from 2013 to 2023, where an electronic search was carried out in the Google Scholar and Scielo databases to identify relevant studies and scientific articles. Through this research, it can be concluded that a healthy and balanced diet, along with maintaining the nutritional and sensory properties of food, has aroused great interest in the world population. Several studies with the use of biotechnology have been emerging around the world in order to identify and improve the nutritional and sensory quality of foods. These food technologies are mainly used in nutritional enrichment and disease prevention, aiming at food quality. Plants are genetically modified, aiming to add new properties with benefits to human health and, as a consequence, the food generated from these plants is preserved for a longer time, avoiding losses resulting from the supply system and the effects of seasonality that generate damage to the quality of the products. foods. In conclusion, biotechnology offers immense potential to improve the sensory characteristics of foods and satisfy consumer preferences, and by understanding and effectively applying biotechnological techniques, the food industry can drive innovation, creating products that are more attractive and in line with the demands of the market and, the search for continuous improvement of the sensory characteristics of foods is fundamental to offer satisfactory gastronomic experiences, conquer consumers' loyalty and remain competitive in the food sector in constant evolution.
Presença de antibióticos na água e seus efeitos tóxicos sobre os ecossistemas e a saúde humana
(Universidade Federal de Goiás, 2023-07-28) Nascimento, Letícia Alves; Oliveira, Gisele Augusto Rodrigues de; Mendonça, Reginaldo Teixeira; Alves, Virgínia Farias; Oliveira, Gisele Augusto Rodrigues de
Medicines are developed for therapeutic purposes, but the presence of these compounds in the environment has led them to be considered emerging contaminants due to their toxicity to biota. Antibiotics have been found in various water matrices in several countries. In view of this, the aim of this study was to analyze the presence of antibiotics in water resources and their toxic effects on aquatic biota and humans. To this end, a bibliographic search was carried out using the main PUBMED, MEDLINE and GOOGLE ACADEMIC databases. The descriptors used were: antibiotics and wastewater, antibiotics and water and human toxicity, antibiotics and water sources and toxicity, antibiotics in wastewater or aquatic toxicity. A total of 150 articles were found, 134 of which were excluded as only 19 met the inclusion criteria, i.e. articles presenting data on the toxicity of antibiotics present in water resources. In terms of geographical distribution, China and Europe accounted for 31.6% of the publications on this subject. It has been observed that antibiotics are present in the most diverse water matrices: lakes, wastewater, groundwater and even drinking water. Among the main classes of antibiotics found in water resources are fluoroquinolones and sulfonamides. Malformations in aquatic organisms and the selection of resistant bacteria were the main effects observed in relation to the presence of antibiotics in water. The results of this review also indicated that the detection and quantification of these compounds in water resources depends on very sensitive analytical methods, since antibiotics are present in low concentrations. Therefore, it can be concluded that the presence of antibiotics in aquatic resources constitutes a serious public and environmental health problem and that preventive and corrective actions are necessary to minimize this source of exposure and generate subsidies for the establishment of exposure limits to these compounds in water resources.
Estudo morfoanatômico, variabilidade sazonal e atividade larvicida dos óleos voláteis das folhas de Campomanesia pubescens (DC.) O. Berg (Myrtaceae)
(Universidade Federal de Goiás, 2023-08-08) Costa, Henrique Margues da; Paula, José Realino de; Fiuza, Tatiana de Sousa; Ferreira, Heleno Dias; Romano, Camila Aline; Fiuza, Tatiana de Sousa; Paula, José Realino de
The aim of this study was to perform from the morpho-anatomical study, physicochemical characterization, chemical composition, seasonal variability and larvicidal activity of the volatile oils of Campomanesia pubescens (DC.) O. Berg leaves. The botanical material was collected in Hidrolândia, Goiás. Morpho-anatomical characterization and phytochemical screening were performed by conventional methods. Pulverized samples were submitted to hydrodistillation in the Clevenger apparatus and the identification of the chemical components of the volatile oils obtained was done by GC-MS. The larvicidal activity was carried out with third-stage larvae of the Aedes aegypti. The leaf blade, the main vein, and the young stem have a uni-stratified epidermis covered by a cuticle. The mesophyll and cortical parenchyma of the main vein and young stem have secretory cavities and idioblasts with crystals. The main rib contains a bicolateral bundle in an open arch. The youngstem has a discontinuous band of sclerenchyma external to the phloem cells. Most volatile oil compounds are spathulenol, caryophyllene oxide, α-macrocarpene, and z-caryophylleneIn phytochemical analysis, tannins, digitalis, flavonoids, and total phenols were detected. The content of volatile compounds was 7.36%, that of total ash was 1.77%, and that of mucilage was 3.52 ml. The volatile oil at the concentration used was inactive against Ae. aegypti larvae.The present study contributes to the taxonomic knowledge of the species and provides parameters for quality control of the plant raw material. This work represents the first description of the chemical compounds and seasonal variability of volatile oils from C. pubescens leaves collected in Goiás state.
Sistemas lipídicos como abordagens tecnológicas para melhora das propriedades biofarmacêuticas de fármacos: levantamento das estratégias e mecanismos descritos na literatura
(Universidade Federal de Goiás, 2023-08-08) Carvalho, Gabriel Cruvinel; Gomes , Lara Ferreira; Silva, Luís Antônio Dantas; Morais, Mariana Cristina de; Alves, Guilherme Liberato; Silva, Luís Antônio Dantas
In the current situation, technological approaches have been sought to optimize the delivery of drugs and guarantee their therapeutic efficacy. The use of lipid systems to improve the bioavailability of orally administered drugs can help correct problems related to the physicochemical properties of the drug, such as low solubility and/or low permeability. The aim of this article was to address lipid systems as technological strategies for improving the biopharmaceutical properties of orally administered drugs. The methodology used was a survey of studies described in the literature over the last 20 years, describing the subject, and especially the benefits and challenges of using lipid systems for orally administered drugs. The theoretical framework therefore presented the parameters for the use of lipid systems as drug carriers, considering their challenges and classifications, highlighting strategies to overcome absorption problems through the use of lipid systems and the main mechanisms, as well as current applications. The technological development of lipid-based drug delivery systems therefore appears to be a promising strategy for improving the biopharmaceutical properties of drugs. With an in-depth understanding of the physicochemical characteristics of drugs, formulation techniques and the gastrointestinal system, it is possible to overcome the challenges of the applicability of lipid formulations and highlight their benefits, which will contribute to significant advances in the field of pharmaceutical technology.
Desenvolvimento e caracterização de carreador lipídico nanoestruturado contendo leflunomida
(Universidade Federal de Goiás, 2023-08-02) Nakamura, Juliana Midori; Silva, Luís Antônio Dantas; Salomão, Mariana Arraes; Hayasaki, Tacio Gonçalves; Silva, Luís Antônio Dantas
Rheumatoid arthritis (RA) is an autoimmune disease characterized by deformation and destruction of peripheral joints. One of the drugs indicated for its treatment is leflunomide (LEF). There are strategies that can be adopted to improve the low aqueous solubility of the drug and minimize its systemic adverse effects (gastrointestinal symptoms, allergic reactions alopecia, skin eruptions, opportunistic infections and arterial hypertension), such as nanoencapsulation and local administration. This project aimed to develop nanostructured lipid carriers (NLC) and to evaluate their physicochemical characteristics, storage stability, kinetic stability and encapsulation efficiency. The method of emulsification and solvent evaporation associated with microfluidization was used. In the physical-chemical characterization and in the stability study, the average size and polydispersity index (PdI) were evaluated through dynamic light scattering (DLS). The kinetic stability of NLC was evaluated through multiple light scattering. The encapsulation efficiency was determined using the indirect method, quantifying the concentration of free drug present in the formulation. The formation of nanometric and homogeneous particles (average size of 213 nm and PdI of 0.239) was observed. The NLC showed low backscattering variation (∆BS), less than 2%, and encapsulation efficiency of 73.3% ± 9.1. The preparation method by emulsification and solvent evaporation obtained systems with appropriate characteristics for future in vitro studies of release and cutaneous permeation of nanoencapsulated LEF.
Identificação molecular de Metarhizium sp. em adultos de Aedes aegypti infectados
(Universidade Federal de Goiás, 2023-07-28) Silva, Fernanda Cristina Soares da; Rodrigues Filho, Juscelino; Luz, Wolf Christian; Rocha, Luiz Fernando Nunes; Rodrigues Filho, Juscelino
The use of entomopathogenic fungi has been an alternative of biorational control to control Aedes aegypti. A device containing mycoinsecticide based on Metarhizium humberi IP 46 for the focal control of A. aegypti adults is in developing. The evaluation of the effectiveness of this device under field conditions can be done with the aid of sensitive methods for detecting IP 46 in diseased mosquitoes captured in the field. To standardizing a methodology to detect IP 46 DNA present in A. aegypti adults after fungal exposure, adults were exposed to IP 46 formulations under laboratory or semi-field conditions for up to 8 or 14 days, respectively. In laboratory testing, the DNA extraction buffers by Saghai-Maroof et al. (1984) (I), Raeder and Broda (1985) (II) and Doyle and Doyle (1987) (III) were evaluated. For the semi-field test, the most efficient buffer in DNA extraction was used in the treated samples. The extractions and amplifications were confirmed by electrophoresis. The TEF-intron region was amplified by PCR using primers EF1T and EF2T. For positive control, use IP 46 mycelium, and for negative control, use unexposed mosquitoes. PCR products with positive amplification were sequenced. The results of the sequencing appreciated that, with buffer I, it was possible to extract DNA from IP 46 present in A. aegypti with incubation of 0, 6 or 8 days at 75% relative humidity (RH) and on 2 or 8 days more of 98% RH. With buffer II, fungal DNA transmission occurred in mosquitoes incubated for 4, 6 or 8 days at 75% RH and for 2, 4, 6 or 8 days at more than 98% RH. IP 46 DNA was obtained from mosquitoes incubated for 2, 6 or 8 days at 75% RH and 1, 2, 4 or 8 days at more than 98% RH, using buffer III. Buffer II showed the most consistent results, so it was used in field trials. With this methodology, it was possible to detect IP 46 in live or dead mosquitoes at different chances of fungal infection. The primers used in the PCR were specific for M. humberi, with no amplification of DNA from othermicroorganisms present in the tested mosquitoes. The buffer described by Raeder and Broda (1985) (II) was the most efficient in the transmission of M. humberi IP 46 DNA in A. aegypti adults, showing potential to be used in a technique for monitoring the effectiveness of the device under conditions field. The evaluation of the protocol in semi-field samples still needs to be done in a more standardized way.
Revisão da literatura sobre o impacto da estimulação da microbiota intestinal com prebióticos e probióticos na integridade cutânea
(Universidade Federal de Goiás, 2023-08-10) Ribeiro, Cibele Valadares; Viana, Narielly Cristina Rezende; Silva , Luís Antônio Dantas; Campos, Geovana Batista de; Chaul, Luíza Toubas; Silva, Luís Antônio Dantas
The manifestation of skin diseases is favored by several factors, among which are: genetic, environmental, psychosocial, and immunological. Considered a key regulator of the immune system, the microbiota acts in gut homeostasis. Changes in the microbiota can contribute to the development of several disease states, including diseases of the integumentary system. Aim: To analyze and present the effects of oral probiotics and prebiotics on the intestinal microbiota and their effects on the maintenance of sq1 kin characteristics, to improve the quality of life of a relevant portion of the world's population that suffers from skin problems. Method: The PubMed, Scielo and CAPES Periodicals Portal database from 2007 to 2023 was used with the following descriptors: “probiotics”, “prebiotics” “skin”, “intestinal microbiota”, “skin care” and “Skin microbiota”. The selected articles were read in full to develop the narration. Results: A healthy balance of gut microbiota is necessary for optimal skin health, resulting in metabolic and immune homeostasis, according to this narrative review. More research has linked changes in gut microbiota composition to the worsening of inflammatory skin conditions such as eczema and psoriasis. Any alteration in the diversity of intestinal microorganisms (dysbiosis) can make the host more vulnerable and interrupt the immune tolerance of the mucosa, influencing the health of the skin. As a result, a variety of dermatological conditions have been linked to intestinal dysbiosis. In the case of dysbiosis, probiotics and prebiotics should be used. Conclusion: Oral probiotics and prebiotics are beneficial in the clinical treatment of skin diseases involving immunological, inflammatory and dysbiotic factors in most of the studies addressed. Both probiotics and prebiotics can help you achieve a healthy complexion while alleviating existing skin conditions. Try them out by incorporating them into your daily skincare routine as well as your diet.
Estudo e compreensão do papel da microbiota cutânea como estratégia para o delineamento de cosméticos antienvelhecimento cutâneo
(Universidade Federal de Goiás, 2023-08-09) Pereira, Dyovanna Pazini; Silva, Shirley Souza; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Silva, Andreia Luiza Pereira; Maciel, Iara Mendes
The skin is the largest organ of the human body and plays a vital role in protecting and regulating the organism. It acts as a physical barrier that shields against injuries and external agents such as bacteria, viruses, pollutants, and solar radiation. The skin also assists in regulating body temperature by acting as thermal insulation and aiding in heat dissipation through perspiration. Additionally, it serves to harbor resident microorganisms with important biological functions, known as the microbiome. Hence, the study of the microbiome, referred to as microbiomics, is on the rise. Over the years, the skin undergoes chemical and physical processes that lead to cellular breakdown, resulting in wrinkles and skin aging. The study of omics sciences is essential for developing personalized strategies to maintain or restore the functions of aging skin. The pharmaceutical professional plays a significant role in this field through research and pharmaceutical care. The objective of this work is to discuss and present the existing relationship between skin aging and the microbiome. We will explore how the skin's microbiota can contribute to the development of strategies, such as the formulation of dermocosmetic products, aimed at mitigating or reversing the damages caused by skin aging. Furthermore, the role played by the pharmaceutical professional in this context will be discussed.
Plataformas de microagulhas para liberação transdérmica de fármacos: uma revisão de literatura
(Universidade Federal de Goiás, 2023-02-10) Lima, Giovanna Almeida; Costa, Hemerson da Silva; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Fontanezi, Bianca Bueno; Cardoso, Gleidson
The skin is an attractive option for the delivery of therapeutic agents such as vaccines, biomolecules, and small molecules. However, the superficial layer of the skin, the stratum corneum, can limit the bioavailability of the pharmaceutical active. Drug penetration through the skin is limited to potent molecules with low molecular weight and moderate fat solubility. The efficacy of a drug depends not only on the properties of the active component, but also on the underlying mechanism of delivery to the body. From this perspective, it is important to consider the optimal method of drug delivery according to the drug's properties. The use of micro-needling enables minimally invasive and highly safe delivery. Microneedling, a transdermal drug delivery system, is growing rapidly due to its advantages in improving patient access to drugs, replacing other routes of administration. The purpose of this literature review was to present microdevices as a means of drug administration through microneedles, as well as the technical characteristics and limitations.
Avaliação do emprego de nanotecnologia à formulações de uso oral de aciclovir
(Universidade Federal de Goiás, 2023-02-09) Santos, Yanka Machado de Paula; Diniz, Danielle Guimarães Almeida; Diniz, Danielle Guimarães Almeida; Silva, Luís Antônio Dantas; Pascoa, Henrique
Acyclovir is an antiviral from the class of nucleoside analogues, which has selective action against Herpes Simplex virus types 1 and 2 (HSV 1 and HSV2) and against Varicella-Zoster virus (VZV), being chosen as the gold standard for treatment of these pathologies. Is used to treat primary herpes by reducing the duration of symptoms, but its low oral absorption resulting from low solubility and permeability implies the frequent use of high doses, which increases the chances of adverse effects and reduces adherence to treatment. Due to the potential characteristics of nanometric systems, this approach has been studied as a strategy for delivering, improving solubility and sustained release of Acyclovir. In view of this, this work aimed to evaluate the use of nanotechnology as a strategy to improve the oral bioavailability of the drug Acyclovir, through a bibliographic survey. A limited number of works were found when it comes to improving oral bioavailability, but the reported nanometric systems proved to be promising, such as the use of polymeric nanoparticles, mucoadhesive polymers, solid lipid nanoparticles, nanoemulsions and the association of different techniques such as the use of nanocrystals. Therefore, nanotechnology has demonstrated results that can help to offer a more effective treatment for the oral use of acyclovir.
Uso de nitratos e nitritos em alimentos e seu impacto na saúde
(Universidade Federal de Goiás, 2023-02-10) Soares, Ana Cláudia da Silva; Silva, Aline Gomes de Moura e; Silva, Aline Gomes de Moura e; Silva, Luís Antônio Dantas; Batista, Rayssa Dias
The substitution of natural foods for processed foods caused by the need to preserve and improve aroma and flavor leads to the ingestion of different types of additives, and some of them can cause great harm to our health. The objective of this work was to investigate the main health consequences of the ingestion of food additives, specifically nitrates and nitrites. This study was carried out through a bibliographical research with a descriptive aspect. Nitrate is one of the main sources of nitrite, which is responsible for preserving food, especially meat products. However, in excessive amounts, these additives tend to produce n-nitroso compounds that are considered harmful. Nitrite and nitrate additives are not strictly controlled in Brazil, and it is precisely for this reason that these additives are used indiscriminately and without the necessary precautions. As a direct alternative to the use of nitrites and nitrates, there is natural or organic curing, which refers to the use of plant extracts such as celery, cabbage, kale, lettuce, spinach and chard, naturally rich in nitrates, in the preparation of cured meat products . Thus concluding that there are several natural alternatives that can replace nitrate and nitrite, without this having any negative interference in food preservation.
Avaliação preliminar da influência do método de preparo e da composição da formulação nas características físico-químicas e estabilidade cinética de carreadores lipídicos nanoestruturados delineados como sistemas para liberação cutânea da leflunomida
(Universidade Federal de Goiás, 2022-08-15) Souza, Wayane Karuena Gomes de; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Mello, Bianca Bueno Fontanezi Dias; Diniz, Danielle Guimarães Almeida
Rheumatoid arthritis (RA) is an autoimmune disease that causes joint degeneration and leads to loss of function in the area. One of the drugs associated with its treatment is leflunomide, a drug that belongs to the isoxazole class and inhibits the enzyme dihydroorotate dehydrogenase. However, it has adverse events that can make its use unfeasible. Thus, topical administration is a promising alternative and for this, the development of nanoformulations is an option. Lipid nanosystems have the advantage of accommodating the drug in their structure and can be developed by different methods. The objective of this work was to evaluate the influence of preparation methods and composition of nanostructured lipid carriers (NCL). Emulsification and solvent evaporation and emulsification melting methods were used, associated with the use of a microfluidizer or sonicator. Parameters were evaluated in relation to size and polydispersity index (PDI), by DLS method, and stability for 7 days was evaluated. The average size of 163.4 nm was obtained, with a PDI of 0.301, but there was a tendency for these parameters to increase during stability, being significant after four days, which may be associated with the nanocarrier formulation. More studies should be carried out in order to optimize production and formulation parameters.
Modelos de aprendizado profundo para avaliação de toxicidade aguda de compostos químicos em aves
(Universidade Federal de Goiás, 2022-08-24) Ramos, Gabrielle Santos; Neves, Bruno Junior; Neves, Bruno Junior; Pereira, Eufrásia de Sousa; Costa, Vinicius Alexandre Fiaia
The modernization of agriculture has provided economic growth as a result of increased productivity. However, this sector has the intense use of pesticides as a practice, which presents a potential risk to the environment and organisms that provide ecological services such as avian species. Considering that birds are seed-dispersing organisms, in vivo acute toxicity studies have been widely used as regulatory criteria for the registration of new pesticides. However, these studies are usually time consuming, expensive and involve ethical issues. Therefore, this work aimed to develop Quantitative Structure Activity Relationship (QSAR) models, based on machine learning, to predict the acute toxicity of chemical compounds in several bird species. Initially, the compilation, integration and preparation of the largest datasets of compounds with data on experimental toxicological properties were performed for the following avian species: A. platyrhynchos, C.virginianus, C. japonica and P. colchicus. Then, a chemical space analysis showed that the prepared datasets share chemical information with each other, and the correlation of toxicological data between species proved to be moderate (with 'r' around 0.68). At the end of this process, QSAR models for regression tasks were generated using Deep Learning methods. Among them, a multitask model based on Feed-Forward Neural Networks (FFNN), capable of predicting the acute toxicity (pDL50) of several bird species simultaneously, was the most predictive, obtaining r values between 0.59 – 0.80 for the test set. The results demonstrate that the multitasking model was able to promote inductive transfer of learning between tasks, that is, between bioassays of each species. Therefore, the generated model represents a new alternative method to the use of animals for the evaluation of acute avian toxicity.
Avaliação da capacidade antioxidante em amostras comerciais de canela por métodos voltamétricos e espectrofotométricos
(Universidade Federal de Goiás, 2022-08-16) Vasconcelos, Igor André Alves; Gil, Eric de Souza; Moreno, Emily Kussmaul Gonçalves; Moreno, Emily Kussmaul Gonçalves; Silva, Giovanna Nascimento de Mello e; Machado, Fábio Bahls
This study aimed to analyze the antioxidant capacity in commercial cinnamon (Cinnamomum sp.), given the growing concern about cellular damage from reactive oxygen species, as well as the desire for healthy and natural food, aiming to compare traditional and innovative methods for the determination of antioxidant capacity. The traditional methods performed were the scavenging of the free radical DPPH (1,1-diphenyl-2-picrylhydrazyl) and the capture of the cation of the radical ABTS (2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate), measured by the spectrophotometry technique. For electrochemical studies, voltammetric techniques such as CV, DPV and SWV were performed and the Electrochemical Index (IE) was calculated. The results of ABTS, DPPH and IE indicated significant differences between the 17 samples. A matrix was calculated, comparing the methods, where it was possible to observe a high statistical correlation (c.a 0.6) between them. It was concluded that the cinnamon samples have high antioxidant capacity and the methodologies used are equivalent.
Inovação no mercado de produtos pet care: revisão da literatura sobre o uso da nanotecnologia em produtos veterinários
(Universidade Federal de Goiás, 2022-08-11) Amorim, Nayra Cristina Melo; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Cappelli, Steffani; Lopes, Angela Ferreira
The veterinary pharmaceutical market has developed in recent years and nanotechnology has been used to optimize and increase the quality of these products. Despite being very promising, it is relevant to understand the perception of consumers about these products. In addition to identifying what benefits nanotechnology can cause when used to develop pet items. The objective of this work was to describe the innovation in the cosmetic segment of products for veterinary care containing nanotechnology, evaluating the perception of caregivers about this market trend. The methodology used was the bibliographic review, through an advanced search in online libraries. The results showed that consumers are willing to invest in products with higher quality and obvious benefits, even at a higher cost. However, the literature review showed a scarcity of published texts on the subject, which shows the little exploration of the sector. In short, it is concluded that the market has much to be explored and developed, it is necessary to invest time and research to produce veterinary articles that meet the requirements of tutors.
Desenvolvimento e validação de método eco-friendly por UV para avaliação de marbofloxacino em comprimidos
(Universidade Federal de Goiás, 2022-08-09) Silva, Thayanara Lorrane Alves da; Kogawa, Ana Carolina; Kogawa, Ana Carolina; Gil, Eric de Souza; Chorilli, Marlus
Fluoroquinolones are a class of antimicrobials, which stand out as an important alternative for the treatment of infections caused by bacteria with a broad spectrum of action. Analytical methods found in the literature related to marbofloxacin (MAR, second-generation fluoroquinolone) show a predominance in the use of toxic organic solvents, which promote the generation of waste, potentially harmful to the environment and the operator. Therefore, na analytical method was proposed for the quantitative analysis of MAR in tablets, by means of spectrophotometry in the ultraviolet (UV) region. Thus, an eco-friendly method was developed and validated aiming speed, low cost, use of less toxic solvents, reducing harmful actions to operators, as well as waste to the environment, in accordance with the principles of Green Analytical Chemistry. The method was developed and validated for the quantification of MAR in tablets by spectrophotometry in the UV region at 296 nm, using quartz cuvettes and purified water: ethanol (80:20, v/v) as a diluent. The method was linear at the concentration of 3-9 μg/mL, with a correlation coefficient of 0.9994 (standard), accurate with a relative standard deviation (RSD) of 1.20 % for the intraday level, 1.68 % for the interday level is 3.26 % for the interanalyst level, exact with an average recovery of 100.39 %, selective in the comparison of standard and sample MAR and robust against changes in wavelength and in the proportion of ethanol used in the preparation of stock solutions. The average content of MAR in tablets, using the validated method, was 103.67 %. Thus, the method can be applied for quantitative assessment of MAR in tablets in an ecologically sustainable, reliable and effective way, being a green analytical option for chemical-pharmaceutical laboratories.
Vigilância das arboviroses nas unidades de saúde de Goiânia: um relato de experiência
(Universidade Federal de Goiás, 2022-04-01) Lima, Camila Rodrigues; Feres, Valeria Christina de Rezende; Feres, Valeria Christina de Rezende; Garcia, Telma Alves; Mendonça, Reginaldo Teixeira
Arboviruses are characterized by a group of vector-borne viral diseases. Arboviruses are a growing public health problem in the world, and to continuously monitor the temporal evolution of these diseases, epidemiological surveillance is responsible for a series of activities, including: notification and timely investigation of suspected cases of Dengue, Chikungunya and Zika. Objective: to report the experience of understanding procedures and protocols for the flow of clinical and laboratory care of patients with suspected infection by: dengue, chikungunya and zika in the basic health units of the city of Goiânia Goiás Methodology: Screening through the flow of clinical care and laboratory tests of patients with suspected dengue, Zika and chikungunya infections in basic health units and referred for molecular testing for the different arboviruses. Results: Through these activities you can observe negative and positive points as well as the result of the research where the 220 samples collected from patients in the health units between 2018 and 2019, 30% were positive for DENV and ZIKV in 2018 and 28.2% of DENV in 2019. Final considerations: This experience report aimed to describe the experience lived as a pharmacy student in the knowledge and application of dengue, Zika and chikungunya care protocols, the reported action develops academic skills whose experience offered in the curriculum of the course is not able to provide, as they are off-campus activities, involve people of different age groups and require direct contact with the population.

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