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Terapias celulares com células encapsuladas para o tratamento do diabetes: uma revisão descritiva
(Universidade Federal de Goiás, 2025-12-03) Martins, Brenda Vitória Silva; Silva, Débora Eduarda Borges da; Silva, Artur Christian Garcia da; Silva, Artur Christian Garcia da; Silva, Luís Antônio Dantas; Ribeiro, Gabriel Brito
Introduction: Type 1 Diabetes Mellitus (T1DM) is a chronic autoimmune disease marked by the destruction of pancreatic beta cells (β), resulting in severe insulin deficiency and subsequent hyperglycemia. With a growing global prevalence and significant impact on public health and sector costs, traditional treatment models demonstrate pertinent limitations. Cell encapsulation emerges as a promising approach, aiming to restore physiological pancreatic function and ensure sustained glycemic control. Objective: To conduct a review on cell encapsulation applied to the treatment of Type 1 Diabetes, analyzing techniques, biomaterials, approaches, and associated challenges. Methodology: This is a descriptive literature review study conducted in the PubMed, Scopus, and Web of Science databases, utilizing the keywords: IPS Cells, Type 1 Diabetes, Encapsulation, Islet Cells and Pancreatic Beta Cells. Research articles published between 2015 and 2025 that addressed the use of encapsulated cells for Diabetes treatment were included. Results: A total of 1,158 articles were initially identified, of which 56 met the inclusion criteria. These studies were subsequently organized into four main thematic categories: fibrosis, vascularization, immunoisolation, and clinical studies. The studies indicate that the use of encapsulated cells can be a promising alternative to overcome the limitations of insulin therapy. The choice of biomaterials and the types of cells used for encapsulation are fundamental to the success of the technique. The studies demonstrated continuous insulin production and sustained long-term glycemic control. Conclusion: Encapsulation of beta cells emerges as an innovative and high-impact therapeutic approach for the treatment of T1DM. With continued advancements in clinical studies, this strategy can be consolidated as a safe, effective, and immunosuppression drug-free treatment.
Caracterização físico-química, mecânica e térmica de potenciais formulações de hidrogéis para a impressão 3D de formas farmacêuticas
(Universidade Federal de Goiás, 2025-12-02) Ferreira, Laryssa Xavier; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Pires, Jader; Fontanezi, Bianca Bueno
3D printing has emerged as a promising technology for the development of customized pharmaceutical dosage forms, enabling the production of complex systems that can be adjusted to therapeutic needs. In this context, hydrogels represent relevant materials due to their high hydration, versatility, and ease of extrusion during the printing process. This work aimed to characterize three hydrogel formulations with potential application in 3D printing of pharmaceutical dosage forms: 50% w/w PVP; PVP and PEG; PVP, PEG and alginate. To this end, chemical analyses were performed using Fourier transform infrared spectroscopy (FTIR), mechanical analyses were performed using thermogravimetry (TGA) and differential thermal analysis (DTA). Each formulation presented specific characteristics related to its composition, such as chemical interaction between components, mechanical response, and dehydration and thermal degradation curves, providing information for the selection of potentially suitable systems for the preparation of hydrogels intended for 3D printing of pharmaceutical forms.
Nanotecnologia aplicada ao desenvolvimento e inovações de produtos cosméticos
(Universidade Federal de Goiás, 2025-12-05) Souza, Débora Izaura de Almeida; Gregório, Mahevi Ferreira; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Alcanfor, Joana D'arc Ximenes; Pascoa, Henrique
Nanotechnology represents one of the most significant advances in modern science, with applications across different areas, including the cosmetic industry. The use of nanobiotechnology in cosmetics has enabled the development of innovative products with greater efficacy, safety, and stability, expanding the potential of bioactive compounds through nanostructured systems. This study aims to analyze the main advances in nanotechnology applied to cosmetics, highlighting its relevance to the development of innovative formulations, as well as its benefits and limitations in practice. The research was conducted through a literature review in recognized scientific databases, including PubMed, Scielo, ScienceDirect, and CAPES Journals, prioritizing studies between 2015 and 2025. The results show that nanotechnology has the potential to revolutionize the cosmetic field, allowing greater skin penetration, controlled release of actives, increased bioavailability, and reduction of adverse effects. However, the use of nanotechnology also raises biosafety and regulatory issues that still require broad discussion.
Formulação de grânulos para mascaramento de sabor amargo de fármacos hidrofílicos para o desenvolvimento de formas farmacêuticas sólidas orais de desintegração instantânea
(Universidade Federal de Goiás, 2025-11-19) Nogueira, Sophia Rogério; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Pascoa, Henrique; Gil, Eric de Souza
Palatability is one of the most critical attributes of orally disintegrating solid dosage forms. Therefore, the exploration of novel techniques to mask the aversive taste of drugs remains in continuous demand. This study focused on the development of effervescent granules for production of orally disintegrating solid dosage forms, specifically orally disintegrating tablets (ODTs), aiming to mask the bitter taste of dipyrone, a widely used drug. The strategy involved the selection of appropriate excipients and the development of effervescent granules as an intermediate step for ODT production, to reduce negative sensory impact. Eleven distinct formulations were prepared, each containing different combinations and proportions of excipients. The methodological process involved individualized weighing of components, standardized for 10 g formulations, with the exception of F1 (which followed quantities from the project reference article) and F8 (adapted due to the use of carnauba microparticles containing a smaller amount of dipyrone). The powders were pre-sieved through a 600 µm mesh to ensure uniformity, while the binders were weighed separately and incorporated using a mortar and pestle. After mixing, the granules were formed by sieving through a 1000 µm mesh, dried in an oven at 40 °C for approximately 3 hours, and stored in sealed bottles within a desiccator containing silica gel. Formulations F6, containing crospovidone and sodium starch glycolate, and F8, with carnauba microparticles, stood out due to superior processability and granule quality. In conclusion, from a pharmaceutic-technological perspective, the developed formulations demonstrated promising potential, with additional stages of physicochemical characterization and sensory evaluation required to verify the effectiveness of the taste-masking approach.
Estratégias nanobiotecnológicas para administração oral de antígenos tumorais: possibilidades e desafios na imunoterapia antitumoral
(Universidade Federal de Goiás, 2025-11-25) Reis, Gabriella Oliveira; Silva, Juliana Inácia Ferreira; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Mendonça, Reginaldo Teixeira; Paula, José Realino de
Introduction: In recent years, anti-tumor immunotherapy has emerged as one of the most promising approaches in cancer treatment, although it still faces challenges related to tumor resistance and the limitations of conventional administration routes. In this context, nanobiotechnological strategies applied to immunotherapy have gained increasing interest, particularly regarding the possibility of oral administration of antigens. Objective: To analyze the scientific and technological advances associated with the use of nanobiotechnological strategies for the development of oral immunotherapies in the oncological context. Methodology: This work consists of a descriptive, qualitative literature review based on articles published between 2015 and 2025 in the PubMed, ScienceDirect, Scopus, and Web of Science databases. The search was conducted using descriptors such as nanobiotechnology, oral administration, tumor antigens, and cancer immunotherapy, combined using the Boolean operator AND. Peer-reviewed studies addressing nanobiotechnological strategies applied to the oral administration of tumor antigens were included, while those that did not directly address this topic were excluded. Data analysis was conducted in a descriptive and interpretive Results: The reviewed literature demonstrated progress in the development of nanostructured systems, such as nanoparticles, liposomes, microspheres, and extracellular vesicles, capable of protecting antigens from enzymatic degradation, modulating the tumor microenvironment, and inducing robust immune responses. Furthermore, the integration of nanotechnology, gut microbiota, and the activation of innate pathways, such as cGAS–STING, has shown promise in overcoming tumor immunosuppression and enhancing therapeutic efficacy. Advances have also been observed in mRNA vaccines, natural peptides, and bacterial delivery systems as innovative platforms for oral immunotherapy. Final considerations: In summary, the findings indicate that the convergence of nanotechnology, immunological biology, and biomaterial engineering is redefining the field of tumor immunotherapy, highlighting the oral route as a viable, safe, and highly promising alternative for the development of more accessible, effective, and personalized cancer therapies.
Avaliação das vias metabólicas associadas à anemia causada por malária
(Universidade Federal de Goiás, 2024-01-15) Oliveira, Geovana Alves de; Silva, Luis Miguel de Souza da; Gardinassi, Luiz Gustavo Araújo; Gardinassi, Luiz Gustavo Araújo; Almeida, Anne Cristine Gomes de; Nonato, Osvaldo Campos dos Santos
Malaria is an infectious disease triggered by exposure to protozoa of the Plasmodium genus, which are transmitted by female Anopheles mosquitoes. The global prevalence of P. falciparum and P. vivax species results in notable morbidity and mortality each year. The mechanisms of parasite-host interaction are not yet fully understood. The relationship between hemoglobin levels and systemic metabolism of patients infected by P. falciparum, P. vivax or coinfection with both species has been studied. This study aimed to investigate which metabolic pathways and metabolites are associated with hemoglobin levels during malaria. We conducted a search for public metabolomic data in the Metabolights database, whose cohorts included patients with malaria caused by P. falciparum, P. vivax or coinfection by both species. Metabolomics data acquired by liquid chromatography – mass spectrometry (LC-MS) were processed, filtered, transformed, and normalized. We used the R statistical and graphical computing environment to analyze the association between hemoglobin levels and metabolites. In the mummichog program, various metabolites and metabolic pathways were significantly associated with hemoglobin levels in each group. We verified that the linoleate metabolism was significant in infections by both Plasmodium species, suggesting an important role for this pathway for malarial anemia. These findings promote the search for biomarkers of disease severity and potential therapeutic targets for malaria.
Dissolução intrínseca: uma importante metodologia na caracterização físico-química de fármacos
(Universidade Federal de Goiás, 2024-01-18) Alves, Flávio Ricardo Bueno; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Flores, Caroline Luiza Martins; Pires, Jader
In the production process of a medicine, so that it can be made available for sale, several regulated steps are possible to be strictly followed, from research into the physicochemical properties of the medicine until its release. In the development cycle of a drug, tests and analytical trials are carried out, from the active pharmaceutical ingredient (IFA) to the finished product. Among the tests carried out with IFA there is an intrinsic dissolution, which is capable of providing results of interest for the quality control of raw materials, development of a new formulation, bioexemption studies and display of in-vitro in-vivo results. Therefore, despite not being a new methodology, it has great potential for application in research and the pharmaceutical industry.
Preparo de micropartículas lipídicas de cera de carnaúba como estratégia tecnológica visando o mascaramento de sabor desagradável de fármacos hidrossolúveis
(Universidade Federal de Goiás, 2025-06-06) Tavares, Layla Lopes; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Pascoa, Henrique; Freitas, Leticia Silva Oliveira
The bitter taste present in many drugs can compromise therapeutic adherence, especially among children and the elderly, as the unpleasant flavor can make medication administration more difficult. Therefore, taste masking becomes a viable strategy to improve treatment adherence and acceptance, and consequently, the quality of the pharmaceutical product. In this context, the present study aimed to develop microparticulate systems as a lipid-based platform for masking the unpleasant taste of water-soluble drugs. Carnauba wax was used as the lipid excipient, and the frozen emulsion method was employed as the taste-masking technique. The method was based on the preparation of two immiscible phases: a lipid phase, composed of carnauba wax and dipyrone, and an aqueous phase, consisting of Milli-Q water and the surfactant Tween 80. The aqueous phase was heated to 90 °C on a heating plate under stirring with a mechanical agitator, and once this temperature was reached, the lipid phase was melted on a separate heating plate. Subsequently, the lipid phase was poured into the aqueous phase under stirring. Two different homogenization methods were tested during this step: (i) using an Ultra-Turrax operating at 10,000 rpm for 1 minute, and (ii) a mechanical agitator A-20 operating at 3,500 rpm for 1 minute. After homogenization, cold water was added to the dispersion, which was stirred again for 1 minute. Finally, the mixture was kept under magnetic stirring for an additional 15 minutes. The microparticles obtained were characterized in terms of morphology, using stereomicroscopy and optical microscopy, and in terms of particle size distribution, using a sieve shaker. The microparticles produced with the Ultra-Turrax showed predominantly spherical morphology but exhibited a widely divergent bimodal size distribution, with higher concentrations retained in the 600 μm and 45 μm sieves. In contrast, the microparticles obtained using the A-20 mechanical agitator displayed less spherical morphology, but a more consistent bimodal size distribution, with concentrations in the 150 μm and 45 μm sieves. Thus, although carnauba wax and the frozen emulsion method proved effective in forming microparticulate systems, further testing is necessary to improve the uniformity and sphericity of the microparticles.
Atividade leishmanicida do nerolidol e de um surfactante: associação com rigidez de membrana em Leishmania amazonensis e macrófagos infectados
(Universidade Federal de Goiás, 2025-06-06) Crosara, Nathalia Fogaça dos Santos; Borges, Ellyêssa do Nascimento; Alonso, Antônio; Alonso, Antônio; Marquezin, Cássia Alessandra; Silva, Kleber Santiago Freitas e
Leishmaniasis is a neglected parasitic disease that affects millions of people, especially in tropical regions such as Brazil. Transmitted by sand flies, currently available treatments have important limitations, such as high toxicity, high cost and increased parasite resistance. In view of this scenario, the leishmanicidal effects of nerolidol, a natural sesquiterpene, and the zwitterionic surfactant HPS (N-hexadecyl-N,N-dimethyl-3-ammonium-1-propanesulfonate) against Leishmania amazonensis were investigated. Using the electron paramagnetic resonance (EPR) spectroscopy of spin labeling, it was found that both compounds altered the fluidity of the parasite's plasma membrane, promoting an initial increase followed by rigidity after 24 h of exposure. IC50 values ??were estimated at approximately 74 ?M for nerolidol and 10 ?M for HPS. At concentrations ranging from one to three times their respective IC50 values, they induced significant membrane stiffness, attributed to the formation of reactive oxygen species (ROS), with consequent lipid peroxidation and/or protein oxidation. In uninfected J774A.1 macrophages, no membrane stiffening was observed, possibly due to the protective action of endogenous nitric oxide, capable of neutralizing oxidative stress. On the other hand, in macrophages previously infected with Leishmania, there was an increase in membrane stiffness after 72 h of treatment, suggesting that the infection sensitizes these cells to oxidative damage. To complement the evaluation of the selectivity and safety of the compounds, their cytotoxicity in macrophages derived from the THP-1 cell line, a more representative model of the human immune system in a state of differentiation, was also analyzed. In this approach, it was observed that the mean CC50 values ??were approximately nine times higher than those recorded in J774A.1 cells, indicating lower toxicity in a model of greater physiological relevance and reinforcing the selective profile of the compounds for the parasite. The data obtained indicate that the antileishmanial activity of nerolidol and HPS is strongly associated with their direct action on the parasite's plasma membrane. The initial increase in fluidity may favor ionic leakage and depolarization of the cell membrane, causing mitochondrial hyperpolarization and increased ROS production, which triggers a sequence of events, including membrane rigidification, resulting in irreversible structural damage and death of the parasite.
Uso da Cannabis sativa no tratamento do mal de Parkinson
(Universidade Federal de Goiás, 2025-06-09) Silva, Kamyla Martins e; Camargo, Leandra Borges de; Menegatti, Ricardo; Menegatti, Ricardo; Paula, José Realino de; Mendonça, Reginaldo Teixeira
Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by impaired motor control and a substantial decline in patients’ quality of life. Conventional treatments, such as levodopa, are effective in alleviating motor symptoms; however, their efficacy often diminishes over time and they are frequently associated with significant side effects. In this context, the therapeutic use of medical cannabis has emerged as a potential alternative. This study aims to review the current scientific literature regarding the effects of cannabinoids in the treatment of PD, with a focus on their therapeutic potential and possible neuropsychiatric implications. Clinical trials, systematic reviews, and recent peer-reviewed publications were analyzed. The findings suggest that cannabinoids—particularly cannabidiol (CBD)—exhibit neuroprotective, anxiolytic, and anti-inflammatory properties. These effects may contribute to the alleviation of symptoms such as tremors, muscle rigidity, sleep disturbances, and anxiety. Nevertheless, the available studies often suffer from methodological limitations, and there is a pressing need for more rigorous randomized controlled trials (RCTs) to determine the long-term efficacy and safety of cannabinoid-based therapies in PD. In conclusion, while medical cannabis shows therapeutic promise in the management of Parkinson’s disease, its use should be considered with caution, grounded in robust scientific evidence and guided by specialized medical supervision.
Fast and new microbiological method for evaluating the potency of marbofloxacin based-tablets
(Universidade Federal de Goiás, 2023-12-07) Ferreira, Raissa Gabrielle Lima; Torres, Ieda Maria Sapateiro; Kogawa, Ana Carolina; Kogawa, Ana Carolina; Silva, Luís Antônio Dantas; Gil, Eric de Souza
Marbofloxacin (MAR) is an antimicrobial belonging to the fluoroquinolone class and is marketed in the form of tablets. There is no microbiological monograph in official compendia to evaluate the potency of the final product. Furthermore, the literature is scarce in this context. Objective: The objective is to develop and validate a microbiological method by turbidimetry to evaluate the potency of MAR-based tablets, based on the principles of green analytical chemistry. Methods: Purified water–ethanol (9 þ 1, v/v) was used as diluent to prepare the MAR solutions at concentrations of 0.25, 0.8, and 2.56 mg/mL, brain heart infusion (BHI) broth as culture media, E. coli American Type Culture Collection (ATCC) 25922 at 10% and incubation in a shaker at 37C for 4 h were used in the method. Results: The method was: linear in the range of 0.25 to 2.56 mg/mL; selective against the sample adjuvants; precise (intra-day RSD 2.26%, inter-day RSD 3.49%, and inter-analyst RSD 3.59%); accurate with a recovery of 100.20%; and robust against changes in culture medium volume in the tube, shaker rotation, and incubation temperature in the shaker. The potency of MAR tablets was 96.98% using the proposed method. Conclusion: The turbidimetric method developed is a new, fast, and optimized option to the routine QC of MAR in tablets, since it allows the evaluation of the drug’s potency in the final product and can be used to complement the results of the physicochemical analysis, in addition to being a green and sustainable alternative.
O uso da nanotecnologia na fotoestabilização da avobenzona
(Universidade Federal de Goiás, 2024-01-22) Feres, Juliana Rezende Vilela; Diniz, Danielle Guimarães Almeida; Diniz, Danielle Guimarães Almeida; Pascoa, Henrique; Santos, Yanka Machado de Paula
The ultraviolet light reaching the Earth's surface can cause irreparable damage to the skin, ranging from premature aging and sunburns to cancer. To prevent this from happening, formulations have been developed with the aim of blocking the penetration of radiation into the deeper layers of the skin, known as sunscreens. The active components present in these products are ultraviolet (UV) filters, which can either reflect and disperse UV rays, as is the case with inorganic filters, or absorb the rays and release energy in the form of heat or in the visible light spectrum. However, a portion of UV filters exhibits photoinstability issues, leading to a decrease in the formulation's effectiveness, evidenced by a reduction in the SPF value. The discovery of the photodegradation process has led to the incorporation of new technologies in formulation development, whether through the addition of antioxidant substances, such as omega-3 and resveratrol, or through the adoption of nanotechnology, using solid lipid nanoparticles (SLNs), liposomes, inorganic nanoparticles, and polymeric nanoparticles. One of the most widely used UV filters in sunscreens is avobenzone, which, despite its excellent potential for absorbing UV radiation, has an unstable chemical structure, requiring protective mechanisms in the formulation, thus becoming the subject of various studies. Therefore, the incorporation of nanotechnological techniques into formulations containing avobenzone is promising for the development of more effective sunscreens. The objective of this work was to compile information about the incorporation mechanisms of avobenzone in sunscreens in order to improve the stability of the molecule.
Sistemas lipídicos para liberação de fármacos no sistema nervoso central: estratégias de delivery baseada na estrutura e composição dos lipídios cerebrais
(Universidade Federal de Goiás, 2024-01-19) Fleury, Suzana Grzelak Borges Reis; Vieira, Thalyta Lopes; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Hayasaki , Tácio Gonçalves; Salomão, Mariana Arraes
The pharmacological treatment of neurological disorders has proven to be essential in controlling symptoms of central nervous system (CNS) diseases. Although effective, the low aqueous solubility of some lipophilic drugs that act on the CNS limits their absorption and bioavailability at the intended site. To overcome these limitations, different technological approaches have been explored for lipid-based drug delivery. These systems are designed to increase the solubility of the active compound, facilitate intestinal absorption, and avoid pre-systemic metabolism, thereby improving oral bioavailability. Advances in the development of these systems may also enable the exploration of new target-directed therapies by delivering drug molecules precisely where they are needed, which is crucial for CNS-related pathologies. This study focuses on exploring the lipid composition of the brain, with an emphasis on polyunsaturated fatty acids that show potential for interacting with nervous tissue to design lipid-based systems for brain drug delivery. Thus, the development of lipid-based drug delivery systems modeled after the brain's composition appears as a promising strategy to optimize pharmacokinetics, targeting, and efficacy in the treatment of CNS diseases.
Avanços da nanotecnologia no desenvolvimento de dermocosméticos: um enfoque nas estratégias tecnológicas de produtos antienvelhecimento
(Universidade Federal de Goiás, 2024-01-17) Silva, Millena Martins Oliveira; Silva, Luís Antônio Dantas; Oliveira, Nathalia Côrrea de Almeida; Salomão, Mariana Arraes; Silva, Luís Antônio Dantas
The skin plays a crucial role in personal image and aesthetics and is susceptible to the manifestation of signs of aging. Therefore, the search for innovative solutions to prevent and mitigate this physiological phenomenon has increased. In this context, dermocosmetic products with anti-aging action stand out for being economical, easy to apply, and for the absence of associated injuries when compared to other interventions. However, the effectiveness of dermocosmetics is compromised by the complexity of skin penetration, attributed to skin stratification and the dimensions of dermocosmetic actives. Thus, nanotechnological strategies applied to dermocosmetics assume significant relevance, exerting a substantial impact on improving their efficacy. This study will provide a detailed and focused exposition of nanotechnological systems, highlighting their multifunctional properties and their application in the effective encapsulation of dermocosmetic actives with anti-aging action. The approach aims to demonstrate how nanotechnology can enhance the effectiveness of skin treatments by overcoming obstacles related to skin penetration, stability of dermocosmetics actives, and control of their release.
Estudo da eficiência de Processos Oxidativos Avançados (POAs) para a degradação de contaminantes emergentes
(Universidade Federal de Goiás, 2023-12-18) Silva, Ludmila Lorrane Martins; Teixeira, Guilhermina Ferreira; Teixeira, Guilhermina Ferreira; Colmati Junior, Flavio; Oliveira, Gisele Augusto Rodrigues de
To explore methods to contribute to the access of potable water for the population, this work employed Advanced Oxidative Processes (AOPs) for the recovery of aquatic effluents contaminated by contaminants of emerging concern (CECs). The chosen methods were photocatalysis, photolysis, and electrolysis, and the contaminants studied were the Brilliant Blue dye and the drugs Paracetamol and Ibuprofen. Photocatalysis was conducted using an aquarium purifier equipped with an 18 W UV lamp as a photoreactor, and sodium niobate compound as a photocatalyst. Regarding the degradation of the dye, the photocatalytic process showed an efficiency of over 90%, while for the degradation of the drug Paracetamol, the efficiency was approximately 54%. When photolysis was employed at pH=9 for the degradation of Paracetamol, the efficiency was around 75%. In terms of electrolysis, when working with a Paracetamol solution with a concentration of 10 mg.L-1 and NaCl 0.05 mol.L -1 as an electrolyte, it was observed that after 5 minutes of the experiment, the process efficiency was around 90%. However, when the Paracetamol concentration increased to 20 mg.L-1 , the same degradation efficiency occurred after 15 minutes of testing. Regarding Ibuprofen, due to its low solubility in water, the results obtained were inconclusive.
Estratégias nanobiotecnológicas para tratamento da artrite reumatoide: uma revisão integrativa
(Universidade Federal de Goiás, 2024-01-17) Freitas, Leticia Silva Oliveira; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Nascimento, Thais Leite; Hayasaki, Tacio Gonçalves
Rheumatoid arthritis (RA) is a systemic autoimmune pathology characterized by a chronic inflammatory process that affects the synovial tissue of the joints, significantly compromising the quality and life expectancy of patients. Conventional treatment, composed of nonsteroidal anti-inflammatory drugs (NSAIDs), glucocorticoids (GCs), conventional synthetic disease-modifying antirheumatic drugs (csDMARDs), targeted synthetic DMARDs, and biological DMARDs, faces considerable challenges. These include the high cost associated with the development of biological agents, frequent administration and high doses, severe adverse events due to lack of specificity and non-selective immunosuppression, low efficacy in some patients, short half-life of drugs, and limited understanding of the mechanisms of action of DMARDs. In this scenario, nanotechnology emerges as a promising approach to address the limitations of conventional treatment. It offers new perspectives by providing specificity to the joints through active and passive targeting strategies, overcoming challenges such as increased dose, administration frequency, and associated adverse effects. Therefore, this review explores different approaches of nanoparticle delivery systems, including polymeric nanoparticles, lipid-based nanoparticles, and metallic nanoparticles, highlighting their strategies and outcomes in rheumatoid arthritis models.
Nanobiotecnologia no desenvolvimento e inovação de biossensores para aplicação na área biomédica: uma revisão da literatura
(Universidade Federal de Goiás, 2024-01-22) Marinho, Millena de Sousa; Vieira, Jessika Chiarelli; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Costa, Sérgio Henrique Nascente; Pereira, Jhon Kennedy Alves
Nanobiotechnology is an interface area between nanotechnology and biotechnology that has shown constant growth in different areas. Among these areas of application is the biomedical area, notably diagnostic medicine, with the development of rapid and accurate diagnostic systems, known as biosensors. These devices present an advance in the diagnosis of diseases, as they are devices that can diagnose the disease from the earliest stages, presenting selectivity, specificity and sensitivity comparable to conventional methods. Furthermore, they have several advantages, particularly highlighting their agility in response time. The purpose of this work was to analyze and describe the use of biosensors in the biomedical area in light of the growing development of new diagnostic and screening tools through a narrative review of the literature. Eight research articles were selected that met the acceptance criteria and were subdivided into four topics, being the biosensor used for glucose monitoring, cancer detection, detection of infectious diseases and detection of hepatotoxicity. It was concluded that biosensor systems will be increasingly present in the routine of pharmacies and clinical analysis laboratories in light of the new RDC 786, of May 5, 2023.
Uso de probióticos em dermocosméticos destinados ao tratamento de pele acneica
(Universidade Federal de Goiás, 2024-01-18) Szikszay, Gabriela de Angelis; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Marreto, Ricardo Neves; Gonçalves, Priscila Bianca Rodrigues da Rocha
Despite being a very prevalent and common skin condition, acne is still a disease whose pathophysiological mechanisms are not very well defined and fully understood. Its conventional therapeutic line presents challenges in its effectiveness and efficiency, in addition to promoting many adverse effects, directly impacting the quality of life of affected individuals. Therefore, new alternatives have been investigated. One of the areas that has been explored to better understand the complexity of acne involves the microbiota and its dysbiosis process. Recent studies point to the importance of maintaining homeostasis of the skin's microbiota for better management of the clinical manifestations of the disease. Among the new therapeutic alternatives that act on the microbiota are topical probiotics. Evidence shows that these have inhibitory activity on acne mechanisms and present a clinical-therapeutic alternative in the management of acne by preventing the growth of pathogenic bacteria and the development of inflammatory processes. However, the therapeutic scenario of topical probiotics is still evolving, requiring greater exploration and clarification on the subject through the promotion of studies that demonstrate better clinical-therapeutic capacity.
Dissolução intrínseca: uma importante metodologia na caracterização físico-química de fármacos
(Universidade Federal de Goiás, 2024-01-18) Alves, Flávio Ricardo Bueno; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Flores, Caroline Luiza Martins; Pires, Jader
In the production process of a medicine, so that it can be made available for sale, several regulated steps are possible to be strictly followed, from research into the physicochemical properties of the medicine until its release. In the development cycle of a drug, tests and analytical trials are carried out, from the active pharmaceutical ingredient (IFA) to the finished product. Among the tests carried out with IFA there is an intrinsic dissolution, which is capable of providing results of interest for the quality control of raw materials, development of a new formulation, bioexemption studies and display of in-vitro in-vivo results. Therefore, despite not being a new methodology, it has great potential for application in research and the pharmaceutical industry.
Prescrição farmacêutica de fitoterápicos: aspectos regulatórios
(Universidade Federal de Goiás, 2024-11-27) Parreira, Ana Paula Gomes; Silva, Luana Inácia Ferreira; Dewulf, Nathalie de Lourdes Souza; Dewulf, Nathalie de Lourdes Souza; Santos, Pierre Alexandre dos; Cardoso, Thaissa Costa
According to The Collegiate Board Resolution (RDC) N°26/2014 of the Brazilian Health Regulatory Agency (Anvisa), herbal medicines are industrialized products made exclusively from the active raw materials of plants, whose efficacy and safety are supported by clinical evidence, ensuring consistent product quality. In Brazil, phytotherapy was integrated into the Brazilian National Health System (SUS) in 2006 through the National Policy on Integrative and Complementary Practices (PNPIC). The development of phytotherapy has favored the regulation of pharmaceutical prescription by the Brazilian Federal Pharmacy Council (CFF) through the resolutions CFF 546/2011, 585/2013, and 586/2013. The study aimed to analyze the regulatory aspects related to the pharmaceutical prescription of herbal medicines. In order to analyze these aspects, an integrative review, a gray literature search and a document analysis were carried out. The databases used for the integrative review were Medline, Lilacs and SciELO, covering the period from January/2008 to December/2023. The gray literature search was undertaken on Google Academic with different combinations of keywords, but only “prescription”, “pharmaceutical” and “herbal medicines” provided pertinent results. The document analysis was conducted on websites of institutions relevant to the pharmaceutical practice: Brazilian National Association of Magistral Pharmacists (Anfarmag), Anvisa, Brazilian Ministry of Health (MS), CFF, Goiás State Health Department, Goiânia City Council, General Secretariat of Government (SGG), Superintendence of Health Surveillance (Suvisa) and Goiânia Health Surveillance Department. The integrative review identified 55 studies, but after reading the title and abstract, none of the results answered the research question. The gray literature search resulted in 13 articles, but only two focused on the regulatory aspects. Sixteen regulations were identified, including one federal law, one state law, one Brazilian Ministry of Health ordinance, five CFF resolutions, five Anvisa RDCs, and three normative instructions. No specific regulations were found in other institutions analyzed, such as SGG, Suvisa, and the Health Surveillance Department of Goiânia. Although the pharmaceutical prescription of herbal medicines requires specific training, the regulation allows the pharmacist to dispense these medicines, highlighting a normative discrepancy that raises concerns about safety and effectivity, as dispensing also affects the patient.

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