Endothelium-dependent vasorelaxant effect of butanolic fraction from camb. leaves in rat thoracic aorta
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2012
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Caryocar brasiliense Camb. “pequi” is a native plant from the Cerrado region of Brazil that contains bioactive components reported
to be antioxidant agents. Previous work has demonstrated that dietary supplementation with pequi decreased the arterial pressure
of volunteer athletes. We found that the crude hydroalcoholic extract (CHE) of C. brasiliense leaves relaxed, in a concentrationdependent
manner, rat aortic rings precontracted with phenylephrine, and that the butanolic fraction (BF) produced an effect
similar to that of the CHE. Aortic relaxation induced by BF was abolished by endothelium removal, by incubation of the nitric oxide
synthase inhibitor L-NAME, or the soluble guanylatecyclase inhibitor ODQ. However, incubation with atropine and pyrilamine
hadno effect on the BF-induced vasorelaxation.Moreover, this effect was not inhibited by indomethacin and tetraethylammonium.
The concentration-response curve to calcium in denuded-endothelium rings was not modified after incubation with BF, and
the vasorelaxation by BF in endothelium-intact rings precontracted with KCl was abolished after incubation with L-NAME.
In addition, administration of BF in anesthetized rats resulted in a reversible hypotension. The results reveal that C. brasiliense
possesses both in vivo and in vitro activities and that the vascular effect of BF involves stimulation of the nitric oxide/cyclic GMP
pathway.
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OLIVEIRA, Lais Moraes de et al. Endothelium-dependent vasorelaxant effect of butanolic fraction from camb. leaves in rat thoracic aorta. Evidence-Based Complementary and Alternative Medicine, New York, v. 2012, p. 1-9, 2012.