Multiplicação “in vitro” de Cattleya x mesquitae pelo método de estiolamento de segmentos caulinares
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Data
2007-03
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Editor
João Batista Duarte
Resumo
The objective of this study was to stimulate the formation
of larger number of shoots and new explants by growth regulators
addition to culture medium of in vitro stem segment orchid
Cattleya x mesquitae. The stem segments were cut off plants
obtained from in vitro seedlings, standardized to 1.0 cm long and
grown at temperature of 25C ± 2C, in the dark, for sixty days.
The culture medium was MS with reduction of macronutrients
by half, containing three concentrations of ANA (0 mg L-1, 0.1
mg L-1, and 2.0 mg L-1) and three concentrations of BAP (0 mg L-1,
0.5 mg L-1, and 2.0 mg L-1). In general, there was a higher shoot
production on the base than on the nodes, what indicates a low
development of the axillary shoots. With the addition of 0.5
mg L-1 of BAP there was an average production of five shoots
per etiolated explant, which represented an increase of up to two
shoots as related to the absence of the growth regulator. A higher
concentration of BAP in the medium (2.0 mg L-1) revealed to be
harmful, causing a 50% reduction in new shoot production. The
absence of light was determinant for stem lengthening, whereas
the application of growth regulators was unnecessary, although a
larger formation of nodes has occurred when 2.0 mg L-1 of ANA
was used.
Descrição
Palavras-chave
Orchidaceae, Orquídea, Micropropagação, Fitorreguladores, Orchid, Micropropragation, Growth regulators
Citação
RAMOS, Tatiana Vieira; CARNEIRO, Iraídes Fernandes. Multiplicação “in vitro” de Cattleya x mesquitae pelo método de estiolamento de segmentos caulinares. Pesquisa Agropecuária Tropical, Goiânia, v. 37, n. 1, p. 10-15, jan./mar. 2007. Disponível em: <https://www.revistas.ufg.br/pat/article/view/1840/1752>.