Immunoproteomic approach of extracellular antigens from Paracoccidioides species reveals exclusive B-cell epitopes

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dc.creatorMoreira, André Luís Elias
dc.creatorOliveira, Milton Adriano Pelli de
dc.creatorSilva, Lana OHara Souza
dc.creatorInácio, Moisés Morais
dc.creatorBailão, Alexandre Melo
dc.creatorRocha, Juliana Alves Parente
dc.creatorLeite, Vanessa Rafaela Milhomem Cruz
dc.creatorPaccez, Juliano Domiraci
dc.creatorSoares, Célia Maria de Almeida
dc.creatorWeber, Simone Schneider
dc.creatorBorges, Clayton Luiz
dc.date.accessioned2024-08-16T13:46:01Z
dc.date.available2024-08-16T13:46:01Z
dc.date.issued2020
dc.description.abstractFungi of the Paracoccidioides genus are the etiological agents of paracoccidioidomycosis (PCM), a systemic mycosis restricted to the countries of Latin America. Currently, the Paracoccidioides complex is represented by Paracoccidioides lutzii, Paracoccidioides americana, Paracoccidioides brasiliensis, Paracoccidioides restrepiensis, and Paracoccidioides venezuelensis. Even with advances in techniques used for diagnosing fungal diseases, high rates of false-positive results for PCM are still presented. Additionally, there is no efficient antigen that can be used to follow up the efficiency of patient treatment. The immunoproteomic is considered a powerful tool for the identification of antigens. In addition, antigens are molecules recognized by the immune system, which make them excellent targets for diagnostic testing of diseases caused by microorganisms. In this vein, we investigated which antigens are secreted by species representing Paracoccidioides complex to increase the spectrum of molecules that could be used for future diagnostic tests, patient follow-up, or PCM therapy. To identify the profile of antigens secreted by Paracoccidioides spp., immunoproteomic approaches were used combining immunoprecipitation, followed by antigen identification by nanoUPLC-MSE -based proteomics. Consequently, it was possible to verify differences in the exoantigen profiles present among the studied species. Through a mass spectrometry approach, it was possible to identify 79 exoantigens in Paracoccidioides species. Using bioinformatics tools, two unique exoantigens in P. lutzii species were identified, as well as 44 epitopes exclusive to the Paracoccidioides complex and 12 unique antigenic sequences that can differentiate between Paracoccidioides species. Therefore, these results demonstrate that Paracoccidioides species have a range of B-cell epitopes exclusive to the complex as well as specific to each Paracoccidioides species. In addition, these analyses allowed us the identification of excellent biomarker candidates for epidemiology screening, diagnosis, patient follow-up, as well as new candidates for PCM therapy.
dc.identifier.citationMOREIRA, André Luís Elias Moreira et al. Immunoproteomic approach of extracellular antigens from Paracoccidioides species reveals exclusive B-cell epitopes. Frontiers in Microbiology, Lausanne, v. 10, e2968, 2020. DOI: 10.3389/fmicb.2019.02968. Disponível em: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02968/full. Acesso em: 8 ago. 2024.
dc.identifier.doi10.3389/fmicb.2019.02968
dc.identifier.issn1664-302X
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/25307
dc.language.isoeng
dc.publisher.countrySuica
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectParacoccidioides spp.
dc.subjectAntigens secreted
dc.subjectEpitopes
dc.subjectDiagnostic
dc.subjectMass spectrometry
dc.titleImmunoproteomic approach of extracellular antigens from Paracoccidioides species reveals exclusive B-cell epitopes
dc.typeArtigo

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