2-(Pyridin-4yl)benzothiazole and Its benzimidazole-analogue: biophysical and in silico studies on their interaction with urease and in vitro anti-Helicobacter pylori activities

Página do item simplificado
dc.creatorPereira, Camila de Paula
dc.creatorLyra, Ana Carolina Fradique de
dc.creatorOliveira, Breno Germano de Freitas
dc.creatorNascimento, Igor José dos Santos
dc.creatorSilva Júnior, Edeildo Ferreira da
dc.creatorAquino, Thiago Mendonça de
dc.creatorSisto, Francesca
dc.creatorFigueiredo, Isis Martins
dc.creatorMartins, Felipe Terra
dc.creatorModolo, Luzia Valentina
dc.creatorSantos, Josué Carinhanha Caldas
dc.creatorFátima, Ângelo de
dc.date.accessioned2023-12-05T10:48:47Z
dc.date.available2023-12-05T10:48:47Z
dc.date.issued2022
dc.description.abstractIn this study, the interaction between benzothiazole (BTA, concentration of a drug required for 50% inhibition in vitro (IC50) = 0.77 mM) and benzimidazole (BIA, IC50 = 2.14 mM) with urease was quantitatively assessed, using UV-Vis, molecular fluorescence, and circular dichroism. The results showed that both compounds interact with urease by a static fluorescence quenching mechanism with a non-fluorescent complex formation. The main forces responsible for stabilizing the supramolecular complex between BTA and urease were hydrophobic while, for BIA, van der Waals interactions and hydrogen bonds were the main ones. Urease conformation changes due to the interaction process were analyzed by circular dichroism and synchronous fluorescence. Besides, a competitive assay with substrate and inhibitors was used to evaluate the preferential urease site of interaction with BTA and BIA. Our experimental and theoretical studies supported that both, BTA and BIA, are mixed-inhibitors of ureases with a slight preference to the active site of such enzymes. Finally, both BTA and BIA showed to possess anti-Helicobacter pylori (one reference strain and six clinical isolates) activity, presenting minimal inhibitory concentration (MIC) values ranging from 38-150 and 20-164 µM, respectively. The urease inhibitors omeprazole and hydroxyurea showed MIC values in the range of 46-185 µM and 1683-> 3366 µM, respectively.
dc.identifier.citationPEREIRA, Camila P. et al. 2-(Pyridin-4yl)benzothiazole and Its benzimidazole-analogue: biophysical and in silico studies on their interaction with urease and in vitro anti-Helicobacter pylori activities. Journal of the Brazilian Chemical Society, Campinas, v. 33, n. 9, p. 1041-1057, 2022. DOI: 10.21577/0103-5053.20220020. Disponível em: https://jbcs.sbq.org.br/default.asp?ed=330. Acesso em: 30 nov. 2023.
dc.identifier.doi10.21577/0103-5053.20220020
dc.identifier.issne- 1678-4790
dc.identifier.issn0103-5053
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/23885
dc.language.isoeng
dc.publisher.countryBrasil
dc.publisher.departmentInstituto de Química - IQ (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectUrease
dc.subjectUrease inhibitor
dc.subjectBenzothiazole
dc.subjectBenzimidazole
dc.subjectDrug-protein interaction
dc.subjectSpectroscopic techniques
dc.title2-(Pyridin-4yl)benzothiazole and Its benzimidazole-analogue: biophysical and in silico studies on their interaction with urease and in vitro anti-Helicobacter pylori activities
dc.typeArtigo

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
Artigo - Camila de Paula Pereira - 2022.pdf
Tamanho:
1.89 MB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

IQ - Artigos publicados em periódicos