Selective antibacterial activity of the cationic peptide PaDBS1R6 against Gram-negative bacteria

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dc.creatorFensterseifer, Isabel Cristina Marques
dc.creatorFelício, Mário Romão
dc.creatorAlves, Eliane Santana Fernandes
dc.creatorCardoso, Marlon Henrique e Silva
dc.creatorTorres, Marcelo Der Torossian
dc.creatorMatos, Carolina Oliveira
dc.creatorSilva, Osmar Nascimento
dc.creatorLu, Timothy K. T.
dc.creatorFreire, Maurício Vilela
dc.creatorNeves, Natan de Carvalho
dc.creatorLiao, Luciano Morais
dc.date.accessioned2024-02-21T11:33:13Z
dc.date.available2024-02-21T11:33:13Z
dc.date.issued2019
dc.description.abstractInfections caused by Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa foremost among them, constitute a major worldwide health problem. Bioinformatics methodologies are being used to rationally design new antimicrobial peptides, a potential alternative for treating these infections. One of the algorithms used to develop antimicrobial peptides is the Joker, which was used to design the peptide PaDBS1R6. This study evaluates the antibacterial activities of PaDBS1R6 in vitro and in vivo, characterizes the peptide interaction to target membranes, and investigates the PaDBS1R6 structure in contact with mimetic vesicles. Moreover, we demonstrate that PaDBS1R6 exhibits selective antimicrobial activity against Gram-negative bacteria. In the presence of negatively charged and zwitterionic lipids the structural arrangement of PaDBS1R6 transits from random coil to α-helix, as characterized by circular dichroism. The tertiary structure of PaDBS1R6 was determined by NMR in zwitterionic dodecylphosphocholine (DPC) micelles. In conclusion, PaDBS1R6 is a candidate for the treatment of nosocomial infections caused by Gram-negative bacteria, as template for producing other antimicrobial agents.
dc.identifier.citationFENSTERSEIFER, Isabel C. M. et al. Selective antibacterial activity of the cationic peptide PaDBS1R6 against Gram-negative bacteria. BBA: biomembranes, Amsterdam, v. 1861, n. 7, p. 1375-1387, 2019. DOI: 10.1016/j.bbamem.2019.03.016. Disponível em: https://www.sciencedirect.com/science/article/pii/S0005273619300744?via%3Dihub. Acesso em: 16 fev. 2024.
dc.identifier.doi10.1016/j.bbamem.2019.03.016
dc.identifier.issne- 1879-2642
dc.identifier.issn0005-2736
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/24398
dc.language.isoeng
dc.publisher.countryHolanda
dc.publisher.departmentInstituto de Química - IQ (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAntimicrobial peptide (AMP)
dc.subjectPaDBS1R6
dc.subjectEscherichia coli
dc.subjectPseudomonas aeruginosa
dc.subjectAntibiotic resistance
dc.subjectDrug design
dc.subjectJoker algorithm
dc.titleSelective antibacterial activity of the cationic peptide PaDBS1R6 against Gram-negative bacteria
dc.typeArtigo

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