Genotipagem com base em sequenciamento de regiões microssatélites para a identificação de clones em Eugenia klotzschiana O. Berg. (Myrtaceae)
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Data
2024-12-05
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Universidade Federal de Goiás
Resumo
Eugenia klotzschiana O. Berg (Myrtaceae) occurs mainly in Cerrado regions, described by its
shrubby size and edible fruits, which have high nutritional value. Studies for this species are
scarce, with limited knowledge about its reproductive habits, although they are an influencing
factor for genetic diversity patterns. Microsatellite markers are quite efficient for analyses of
individual discrimination and genetic diversity. Furthermore, by using the genotyping
technique by sequencing these markers (SSR-GBS), it is possible to obtain information not
only about the size of the alleles, but also about their sequences, with a greater amount of
data compared to the traditional method. The objective of this work was to investigate the
sequences of the amplified fragments and investigate the presence of clones in a population of
Eugenia klotzschiana. For this purpose, sequence data from 22 SSR-Seq loci, previously
obtained with the SSR-GBS pipeline, were used for 37 individuals from a natural population.
Point mutations were observed in 60% of the loci and all loci were polymorphic. The average
number of alleles was 3.1, ranging from 2 to 5 alleles per locus. Nei's genetic diversity (He)
was 0.58, the observed heterozygosity (Ho) was 0.88 and the intrapopulation fixation index
(f) was -0.537, reducing excess heterozygotes. The combined probability of paternity
exclusion (Q) analysis was 0.9998 and the combined probability of identity (Pi) was
(1.09×10-14 ), indicating that a battery of locomotives is adequate for conducting individual
discrimination analyses. The presence of clones in the population was confirmed, forming
eight clonal lineages, with a clonal range of more than 300 meters, offering asexual
reproduction over long distances.
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Pêra do cerrado, Diversidade genética, Reprodução assexuada, Sequenciamento de nova geração, Cerrado pear, Genetic diversity, Asexual reproduction, Next generation sequencing
Citação
BATISTA, Noelia Vitória de Jesus. Genotipagem com base em
sequenciamento de regiões microssatélites para a identificação
de clones em Eugenia klotzschiana O. Berg. (Myrtaceae).
2024. 31 f. Trabalho de Conclusão de Curso (Bacharelado em
Ciências Biológicas) - Instituto de Ciências Biológicas,
Universidade Federal de Goiás, Goiânia, 2024