Genetic diversity in natural populations of Jacaranda decurrens Cham. determined using RAPD and AFLP markers

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dc.creatorBertoni, Bianca Waleria
dc.creatorTelles, Mariana Pires de Campos
dc.creatorGaiosso, Milena Gaion
dc.creatorTorres, Simone Cristina Zampollo
dc.creatorPereira, José Odair
dc.creatorLourenço, Miriam Verginia
dc.creatorFrança, Suzelei de Castro
dc.creatorPereira, Ana Maria Soares
dc.date.accessioned2017-06-05T12:11:48Z
dc.date.available2017-06-05T12:11:48Z
dc.date.issued2010-09
dc.description.abstractJacaranda decurrens (Bignoniaceae) is an endemic species of the Cerrado with validated antitumoral activity. The genetic diversity of six populations of J. decurrens located in the State of São Paulo was determined in this study by using molecular markers for randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). Following optimization of the amplification reaction, 10 selected primers generated 78 reproducible RAPD fragments that were mostly (69.2%) polymorphic. Two hundred and five reproducible AFLP fragments were generated by using four selected primer combinations; 46.3% of these fragments were polymorphic, indicating a considerable level of genetic diversity. Analysis of molecular variance (AMOVA) using these two groups of markers indicated that variability was strongly structured amongst populations. The unweighted pair group method with arithmatic mean (UPGMA) and Pearson’s correlation coefficient (RAPD -0.16, p = 0.2082; AFLP 0.37, p = 0.1006) between genetic matrices and geographic distances suggested that the population structure followed an island model in which a single population of infinite size gave rise to the current populations of J. decurrens, independently of their spatial position. The results of this study indicate that RAPD and AFLP markers were similarly efficient in measuring the genetic variability amongst natural populations of J. decurrens. These data may be useful for developing strategies for the preservation of this medicinal species in the Cerrado.pt_BR
dc.identifier.citationBERTONI, Bianca W.; TELLES, Mariana P. de C.; MALOSSO, Milena G.; TORRES, Simone C. Z.; PEREIRA, José O.; LOURENCO, Mirian V.; FRANCA, Suzilei de C.; PEREIRA, Ana M. S. Genetic diversity in natural populations of Jacaranda decurrens Cham. determined using RAPD and AFLP markers. Genetics and Molecular Biology, Ribeirão Preto, v. 33, n. 3, p. 532-538, Sept. 2010.pt_BR
dc.identifier.doi10.1590/S1415-47572010005000068
dc.identifier.issne- 1678-4685
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/11993
dc.language.isoengpt_BR
dc.publisherSociedade Brasileira de Genéticapt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectBignoniaceaept_BR
dc.subjectCarobinhapt_BR
dc.subjectCerradopt_BR
dc.subjectGermplasm bankpt_BR
dc.subjectPreservationpt_BR
dc.titleGenetic diversity in natural populations of Jacaranda decurrens Cham. determined using RAPD and AFLP markerspt_BR
dc.typeArtigopt_BR

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