Noninvasive intratumoral thermal dose determination during in vivo magnetic nanoparticle hyperthermia: combining surface temperature measurements and computer simulations
Nenhuma Miniatura disponível
Data
2020
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Purpose: Noninvasive thermometry during magnetic nanoparticle hyperthermia (MNH) remains a challenge.
Our pilot study proposes a methodology to determine the noninvasive intratumoral thermal
dose during MNH in the subcutaneous tumor model.
Methods: Two groups of Ehrlich bearing-mice with solid and subcutaneous carcinoma, a control
group (n¼6), and a MNH treated group (n¼4) were investigated. Histopathology was used to evaluate
the percentage of non-viable lesions in the tumor. MNH was performed at 301 kHz and
17.5 kA.m 1, using a multifunctional nanocarrier. Surface temperature measurements were obtained
using an infrared camera, where an ROI with 750 pixels was used for comparison with computer simulations.
Realistic simulations of the bioheat equation were obtained by combining histopathology
intratumoral lesion information and surface temperature agreement of at least 50% of the pixel’s temperature
data calculated and measured at the surface.
Results: One animal of the MNH group showed tumor recurrence, while two others showed complete
tumor remission (monitored for 585 days). Sensitivity analysis of the simulation parameters indicated
low tumor blood perfusion. Numerical simulations indicated, for the animals with complete remission,
an irreversible tissue injury of 91 ± 5% and 100%, while the one with recurrence had a lower value,
56 ± 7%. The computer simulations also revealed the in vivo heat efficiency of the nanocarrier.
Conclusion: A new methodology for determining noninvasively the three-dimensional intratumoral
thermal dose during MNH was developed. The method demonstrates the potential for predicting the
long-term preclinical outcome of animals treated with MNH.
Descrição
Palavras-chave
Thermal nanomedicine, Cancer, Fluorescence molecular tomography, Treatment planning, Photogrammetry
Citação
CAPISTRANO, Gustavo; RODRIGUES, Harley F.; ZUFELATO, Nicholas; GONCALVES, Cristhiane; CARDOSO, Clever G.; SILVEIRA-LACERDA, Elisangela P.; BAKUZIS, Andris F. Noninvasive intratumoral thermal dose determination during in vivo magnetic nanoparticle hyperthermia: combining surface temperature measurements and computer simulations. International Journal of Hyperthermia, London, v. 37, n. 3, p. 120-140, 2020. DOI: 10.1080/02656736.2020.1826583. Disponível em: https://www-tandfonline.ez49.periodicos.capes.gov.br/doi/full/10.1080/02656736.2020.1826583. Acesso em: 11 abr. 2023.