Immobilized horseradish peroxidase on discs of polyvinyl alcohol-glutaraldehyde coated with polyaniline

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dc.creatorCaramori, Samantha Salomão
dc.creatorFernandes, Kátia Flávia
dc.creatorCarvalho Junior, Luiz Bezerra de
dc.date.accessioned2018-10-03T14:54:34Z
dc.date.available2018-10-03T14:54:34Z
dc.date.issued2012
dc.description.abstractDiscs of network polyvinyl alcohol-glutaraldehyde (PVAG) were synthesized and coated with polyaniline (PANI) using glutaraldehyde as a chemical arm (PVAG-PANIG-HRP disc). The best conditions for the immobilization were established as about 1.0 mg mL − 1 of protein, for 60 min and pH 5.5. The soluble enzyme lost all of its activity after incubation at 70 ◦ C for 15 min, whereas the PVAG-PANIG-HRP disc retained about half of the initial activity for pyrogallol. The same PVAG-PANIG- HRP disc was used consecutively three times without any activity lossbut presented 25% of the initial activity after the 7th use. PVAG-PANIG-HRP disc retained approximately 80% and 60% of its initial activity after 60 and 80 days of storage, respectively. Resorcinol, m-cresol, catechol, pyrogallol, α-naphthol, βnaphthol, and 4, 4 -diaminodiphenyl benzidine were efficiently oxidized by the PVAG-PANIG-HRP disc (from about 70% to 90%), and it was less efficient towards aniline, phenol, and 2-nitrosonaphthol.pt_BR
dc.identifier.citationCARAMORI, Samantha Salomão; FERNANDES, Kátia Flávia; CARVALHO JUNIOR, Luiz Bezerra de. Immobilized horseradish peroxidase on discs of polyvinyl alcohol-glutaraldehyde coated with polyaniline. The Scientific World Journal, London, v. 2012, p. 1-8, 2012.pt_BR
dc.identifier.doi10.1100/2012/129706
dc.identifier.issn2356-6140
dc.identifier.issne- 1537-744X
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/16114
dc.language.isoengpt_BR
dc.publisher.countryGra-bretanhapt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleImmobilized horseradish peroxidase on discs of polyvinyl alcohol-glutaraldehyde coated with polyanilinept_BR
dc.typeArtigopt_BR

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