Study on the interaction of 4'-hydroxychalcones and their Mannich derivatives with calf thymus DNA by TLC and spectroscopic methods, a DNA cleavage study
| dc.creator | Rozmer, Zsuzsanna | |
| dc.creator | Valeze, Aline Bernardes | |
| dc.creator | Noda Pérez, Caridad | |
| dc.creator | Perjési, Pál | |
| dc.date.accessioned | 2023-08-25T15:37:02Z | |
| dc.date.available | 2023-08-25T15:37:02Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Background: Phenolic Mannich bases derived from hydroxychalcones show remarkable cytotoxic potencies towards cancer cell lines. However, the exact mechanism of action is still partially uncleared. Objective: Interaction of two hydroxychalcones and their Mannich derivatives with calf thymus DNA (ctDNA) has been investigated. Methods: Thin-layer chromatography and UV-Vis spectroscopic method were used for studying the interaction. The binding constant has been determined by UV-Vis spectrophotometric titration. The DNA cleavage activity of the compounds was studied by agarose gel electrophoresis. Results: Interaction of the compounds with ctDNA exhibited relatively high intrinsic binding constant (4-5x104 M-1). The results indicate existence of weak, non-covalent interactions between the investigated derivatives with ctDNA. Some compounds showed a slight DNA cleavage activity with pBR322. Conclusion: The obtained results provide additional knowledge on the previously documented cytotoxicity against tumor cell lines of the hydroxychalcones and their Mannich-derivatives. | pt_BR |
| dc.identifier.citation | ROZMER, Zsuzsanna; BERNARDES, Aline; PÉREZ, Caridad N.; PERJÉSI, Pal. Study on the interaction of 4'-hydroxychalcones and their Mannich derivatives with calf thymus DNA by TLC and spectroscopic methods, a DNA cleavage study. The Open Medicinal Chemistry Journal, [s. l.], v. 14, p. 122-131, 2020. DOI: 10.2174/1874104502014010122. Disponível em: https://openmedicinalchemistryjournal.com/VOLUME/14/PAGE/122/. Acesso em: 24 ago. 2023. | pt_BR |
| dc.identifier.doi | 10.2174/1874104502014010122 | |
| dc.identifier.issn | e- 1874-1045 | |
| dc.identifier.uri | http://repositorio.bc.ufg.br/handle/ri/23438 | |
| dc.language.iso | eng | pt_BR |
| dc.publisher.country | Holanda | pt_BR |
| dc.publisher.department | Instituto de Química - IQ (RMG) | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.subject | Chalcones | pt_BR |
| dc.subject | Hydroxychalcones | pt_BR |
| dc.subject | Mannich bases | pt_BR |
| dc.subject | DNA binding | pt_BR |
| dc.subject | UV-Vis spectroscopy | pt_BR |
| dc.subject | DNA cleavage | pt_BR |
| dc.title | Study on the interaction of 4'-hydroxychalcones and their Mannich derivatives with calf thymus DNA by TLC and spectroscopic methods, a DNA cleavage study | pt_BR |
| dc.type | Artigo | pt_BR |