The effect of carbon dioxide pneumoperitoneum on the healing colonic anastomosis in rats
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Data
2013
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Resumo
PURPOSE: To investigate the effects of pneumoperitoneum on colonic anastomosis healing.
METHODS: Colonic anastomosis was performed in 120 rats divided into four groups: Group I - pneumoperitoneum before laparotomy,
Group II - pneumoperitoneum after laparorrhaphy, Group III - pneumoperitoneum before laparotomy and after laparorrhaphy, Group
IV – no pneumoperitoneum (control group). Pneumoperitoneum pressure was 5 mmHg. Animals were killed on the 3 rd , 7 th and 14 th
postoperative day. Hhistopathological features, anastomosis breaking strength, collagen histomorphometry and hydroxyproline
concentration were assessed.
RESULTS: Breaking strength between groups: (day 3, p = 0.165; day 7, p = 0.219; day 14, p = 0.539). Histopathology revealed that
group II had, on day 7, less infiltration of mononuclear cells (p = 0.006), greater infiltration of polymorphonuclear cells (p = 0.001)
and greater necrosis (p = 0.001); and on day 14, less fibrosis. Histomorphometry revealed a decrease in collagen in groups I and III
(p < 0.001) on day 7 and an increase in groups I and II on day 14 (p < 0.001). Hydroxyproline concentration was similar for groups on
days 3 (p = 0.152), 7 (p = 0.913) or 14 (p = 0.981).
CONCLUSION: Carbon dioxide does not impair the healing of colonic anastomosis in rats.
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Pneumoperitoneum, Colon, Rats, Artificial, Carbon dioxide, Surgical, Anastomosis, Wound healing
Citação
DURÃES, Leonardo de Castro; FARIAS, Igor Eduardo Caetano de; MORAIS, Pedro Henrique Alves de; OLIVEIRA, Paulo Gonçalves de; CAVALCANTI NETO, Florêncio Figueiredo; LINO JÚNIOR, Ruy de Souza; FERES, Omar; SOUSA, João Batista de. The effect of carbon dioxide pneumoperitoneum on the healing colonic anastomosis in rats. Acta Cirúrgica Brasileira, São Paulo, v. 28, n. 9, p. 670-677, 2013.