Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin
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2020
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Paracoccidioidomycosis is a highly prevalent systemic mycosis in Latin America, caused by fungi of the genus
Paracoccidioides. Copper is essential for eukaryotes and bacteria. This micronutrient is used in many vital bio chemical processes, although metal excess levels can be toxic for organisms. Pathways underlying copper
overload are poorly understood in members of the Paracoccidioides complex. The responses of Paracoccidioides
lutzii yeast cells to copper overload were here evaluated. The results showed that under copper overload, cells
presented a dark brown pigment, identified as melanin. Proteomic analyses identified mainly the accumulation
of proteins related to amino acids metabolism, ergosterol synthesis and melanin production, suggesting that P.
lutzii responds to copper overload by changing aspects of its metabolism and also plasma membrane and cell wall
remodeling. Proteomic data were confirmed by biochemical analysis.
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Fungi, Copper overload, Cellular defense, Proteome
Citação
PORTIS, Igor Godinho et al. Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin. Microbiological Research, Amsterdam, v. 239, e126524, 2020. DOI: 10.1016/j.micres.2020.126524. Disponível em: https://www.sciencedirect.com/science/article/pii/S094450132030392X?via%3Dihub. Acesso em: 22 nov. 2024.