Atividade citotóxica do extrato bruto etanólico de Spermacoce poaya A. Saint-Hilaire (Rubiaceae) em células normais e tumorais in vitro
Data
2011
Título da Revista
ISSN da Revista
Título de Volume
Editor
Vera Lúcia Gomes Klein
Resumo
Spermacoce poaya is a species that belongs to the family Rubiaceae, tribe Spermacoce, and
genus Borreria, popularly known in Brazil as “poaia-do-campo”, “poaia-rasteira”, and “poaia-do-arador”.
The infusion is used in cases of bronchitis, asthma, and pneumonia. So far, no phytochemical, pharmacological,
and/or toxicological studies about this species have been found in the literature. In the present
study, we investigated the cytotoxic activity of S. poaya crude extract using the colorimetric assays MTT
and trypan blue. The cellular strains HFF-1 and K-562 were exposed for 24 h to different concentrations
of the ethanolic crude extract of S. poaya leaves. The results showed that, for normal cells (HFF-1), the crude extract is antiproliferative at the concentrations of 0.001 mg.mL-¹ and 0.1 mg.mL-¹; on the
other hand, for the tumor cells (K-562), it showed no cytotoxic effect at the tested concentrations. It is
necessary to continue the studies about this plant using other tumor cell lines and, mainly, normal cells,
so that its cytotoxic effect can be testified.
Descrição
v. 8, n. 1, p. 25-32, 2011.
Palavras-chave
Azul de tripano, Citotoxicidade celular, MTT, Proliferação celular, Spermacoce poaya
Citação
MELLO, Francyelli Mariana dos Santos; PIRES, Wanessa Carvalho; PEREIRA, Flávia de Castro; LIMA, Aliny Pereira; VILANOVA-COSTA, Cesar Augusto Sam Tiago; KATO, Lucilia; SILVEIRA-LACERDA, Elisângela de Paula. Atividade citotóxica do extrato bruto etanólico de Spermacoce poaya A. Saint-Hilaire (Rubiaceae) em células normais e tumorais in vitro. Revista de Biologia Neotropical, v. 8, n. 1, p. 25-32, 2011. Disponível em: <http://www.revistas.ufg.br/index.php/RBN/article/view/8116/10051>.