Veterinary application of LAMP: a simple and visual detection tool for feline sporotrichosis

Abstract

Early diagnosis of sporotrichosis, along with the surveillance of fungal circulation in both human and domestic animal populations, is critical for global health. Although the gold standard method is microbiological culture followed by microscopic analysis of the fungus, this methodology requires a long period and skilled professionals for pathogen identification. We developed a molecular assay and conducted a preliminary evaluation using clinical samples using Loop-Mediated Isothermal Amplification (LAMP) technology that detects Sporothrix schenckii and Sporothrix brasiliensis fungi, with results in 30 min. Results are visually interpreted and do not require DNA extraction, making it a promising tool for point-of-care diagnosis in veterinary settings.