Assessment of the cytotoxic, genotoxic, and antigenotoxic activities of Celtis iguanaea (Jacq.) in mice
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Data
2013
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Resumo
Ethnobotanical surveys of Cerrado native plants show that leaves of Celtis iguanaea (Jacq.) Sargent
(Cannabaceae), popularly known in Brazil as “esporão de galo”, are used in folk medicine for body pain,
asthma, cramps, poor digestion, urinary infection, kidney dysfunctions, as well as a stimulant and diuretic.
This work aimed at evaluating possible C. iguanaea aqueous leaf extract (CALE) cytotoxicity, genotoxicity,
and antigenotoxicity using the mouse bone marrow micronucleous test. To assess CALE genotoxicity, Swiss
mice were orally treated with three different extract concentrations (100, 300, and 500 mgkg -1 ). To evaluate its
antigenotoxicity, the same doses were used simultaneously with a single i.p. dose of mitomycin C (MMC,
4mg.kg -1 ). The frequencies of micronucleated polychromatic erythrocytes (MNPCE) were evaluated
24 h and 48 h after administration except for the negative control (24 h). Genotoxicity was evaluated using
the frequency of micronucleated polychromatic erythrocytes (MNPCE), whereas cytotoxicity was assessed by
the polychromatic and normochromatic erythrocytes ratio (PCE/NCE). The results showed that CALE did not
exhibit a significant reduction in the PCE/NCE ratio, neither a considerable increase in the frequency of MNPCE.
Nonetheless, CALE reduced bone marrow toxicity (increased PCE/NCE ratio) and decreased the micronuclei
frequency induced by MMC. We can conclude that CALE presented no cytotoxic and genotoxic effects, but
showed antigenotoxic and anticytotoxic actions under the experimental conditions applied in this study.
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Cytotoxicity, Medicinal plant, Genotoxicity, Mice, Medicinal plant, Citotoxicidade, Ggenotoxicidade, Camundongos, Planta medicinal
Citação
BORGES, Flávio F. V.; MACHADO, Thiago M.; CUNHA, Kênya S.; PEREIRA, Karla C.; Costa, Elson A.; PAULA, José R. de; LEE, Chen-Chen. Assessment of cytotoxic, genotoxic and antigenotoxic activities of Celtis iguanaea (Jacq) in mice. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 85, n. 3, p. 225-233, 2013.