Dengue diagnosis on laser printed microzones using smartphone-based detection and multivariate image analysis

Oliveira, Karoliny Almeida; Damasceno, Deangelis; Teixeira, Cristina Rodrigues de Oliveira; Silveira, Lucimeire Antonelli da; Oliveira, Anselmo Elcana de; Coltro, Wendell Karlos Tomazelli

Dengue diagnosis on laser printed microzones using smartphone-based detection and multivariate image analysis

Data

2016

Autores

Oliveira, Karoliny Almeida

Damasceno, Deangelis

Teixeira, Cristina Rodrigues de Oliveira

Silveira, Lucimeire Antonelli da

Oliveira, Anselmo Elcana de

Coltro, Wendell Karlos Tomazelli

Resumo

This study describes the use of smartphone-based detection and multivariate image analysis (MIA) to perform dengue diagnosis on laser printed microzones. Toner-based microplates containing 96 microzones were fabricated on a transparency film surface through a single laser printing step. Enzyme-linked-immunosorbent assay (ELISA) experiments were then carried out to detect both immunoglobulin M (IgM) and G (IgG) antibodies in serum samples collected from patients infected and not infected with dengue virus. Principal component analysis (PCA) of ELISA digital image data acquired with a smartphone revealed good discrimination between samples from infected and non-infected patients.

Citação

OLIVEIRA, Karoliny A. et al. Dengue diagnosis on laser printed microzones using smartphone-based detection and multivariate image analysis. Analytical Methods, London, v. 8, n. 35, p. 6506-6511, 2016. DOI: 10.1039/C6AY01262K. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2016/AY/C6AY01262K. Acesso em: 14 jun. 2023.