The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis
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2019
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Resumo
Angiogenesis is a fundamental physiological process with strong implications in tissue
homeostasis. Animal models helping to identify how angiogenesis is regulated are fundamental to answer many
biological questions. Chick embryo chorioallantoic membrane (CAM) assay is one of the most employed
methods to study angiogenesis. In this study we applied a scientometric approach to evaluate the employment
of CAM assay in published articles. Temporal trends indicated that CAM assay was the preferred method to
investigate angiogenesis over time. The publications had a significant number of citations and the impact factor
of journals publishing articles is relevant for the scientific community. A total of 52 different research areas
have articles published using this particular technique. Oncology is the research field in which CAM assay was
mostly used. Accordingly, tumor-derived cell lines were the most frequent sample tested on CAM. We also
identified that 73,6% of articles published used only CAM assay to answer questions concerning angiogenesis.
We concluded that although the CAM assay is a classical approach, that does not need so much infrastructure
and financial support to be performed, it is a well-accepted technique by the scientific community. In addition,
this methodology has gain attention in scientific community because no pain is experienced by the chick and
they are minor ethical concerns to employ this method. Moreover, this data can help researchers who are
unfamiliar with the CAM assay to identify if this particular method is suitable for their research.
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Alternative method, Tumor vascularization, Angiogenesis, Oncology, Bibliometric analysis, CAM assay, Métodos alternativos, Vascularização tumoral, Angiogenese, Oncologia, Análise bibliométrica, Ensaio CAM
Citação
PRADO, Angélica Daiane Lemos do; BAILÃO, Elisa Flávia Luiz Cardoso; NABOUT, João Carlos; RABACHINI, Tatiana; REIS, Paulo Roberto de; GONÇALVES, Pablo José; ALMEIDA, Luciane Madureira. The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis. Bioscience Journal, Uberlândia, v. 35, n. 4, p. 1262-1275, 2019. DOI: 10.14393/BJ-v35n4a2019-42777. Disponível em: https://seer.ufu.br/index.php/biosciencejournal/article/view/42777. Acesso em: 5 maio 2023.