An efficient Agrobacterium tumefaciens-mediated transformation method for Simplicillium subtropicum (Hypocreales: Cordycipitaceae)

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dc.creatorOliveira, Nicolau Sbaraini
dc.creatorTomazett, Mariana Vieira
dc.creatorPenteriche, Augusto Bartz
dc.creatorGonçales, Relber Aguiar
dc.creatorCamargo, Matheus da Silva
dc.creatorBailão, Alexandre Melo
dc.creatorBorges, Clayton Luiz
dc.creatorSchrank, Augusto
dc.creatorSoares, Célia Maria de Almeida
dc.creatorStaats, Charley Christian
dc.date.accessioned2024-08-16T12:56:19Z
dc.date.available2024-08-16T12:56:19Z
dc.date.issued2021
dc.description.abstractFilamentous fungi are the organisms of choice for most industrial biotechnology. Some species can produce a variety of secondary metabolites and enzymes of commercial interest, and the production of valuable molecules has been enhanced through different molecular tools. Methods for genetic manipulation and transformation have been essential for the optimization of these organisms. The genus Simplicillium has attracted increased attention given several potential biotechnological applications. The Simplicillium genus harbors several entomopathogenic species and some isolates have been explored for bioremediation of heavy metal contaminants. Furthermore, the myriad of secondary metabolites isolated from Simplicillium spp. render these organisms as ideal targets for deep exploration and further biotechnological mining possibilities. However, the lack of molecular tools hampered the exploration of this genus. Thus, an Agrobacterium tumefaciens-mediated transformation method was established for Simplicillium subtropicum, employing the far-red fluorescent protein TURBOFP635/Katushka, as a visual marker, and the selection marker SUR gene, that confers resistance to chlorimuron ethyl. Notably, one round of transformation using the established method yielded almost 400 chlorimuron resistant isolates. Furthermore, these transformants displayed mitotic stability for, at least, five generations. We anticipate that this method can be useful for deep molecular exploration and improvement of strains in the Simplicillium genus.
dc.identifier.citationSBARAINI, Nicolau et al. An efficient Agrobacterium tumefaciens-mediated transformation method for Simplicillium subtropicum (Hypocreales: Cordycipitaceae). Genetics and Molecular Biology, Ribeirão Preto, v. 44, n. 3, e20210073, 2021. DOI: 10.1590/1678-4685-GMB-2021-0073. Disponível em: https://www.scielo.br/j/gmb/a/6qhqqN3qCD3Ljdpw5VXZm4n/. Acesso em: 8 ago. 2024.
dc.identifier.doi10.1590/1678-4685-GMB-2021-0073
dc.identifier.issn1415-4757
dc.identifier.issne- 1678-4685
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/25299
dc.language.isoeng
dc.publisher.countryBrasil
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectSimplicillium
dc.subjectSimplicillium subtropicum
dc.subjectAgrobacterium tumefaciens
dc.subjectKatushka
dc.subjectAgrobacterium tumefaciens-mediated transformation
dc.titleAn efficient Agrobacterium tumefaciens-mediated transformation method for Simplicillium subtropicum (Hypocreales: Cordycipitaceae)
dc.typeArtigo

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