Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
| dc.creator | Britto, Constança | |
| dc.creator | Silveira, Celeste | |
| dc.creator | Cardoso, Maria Angelica | |
| dc.creator | Marques, Patricia | |
| dc.creator | Ostermayer, Alejandro Luquetti | |
| dc.creator | Macêdo, Vanize | |
| dc.creator | Fernandes, Octavio | |
| dc.date.accessioned | 2018-03-28T11:49:41Z | |
| dc.date.available | 2018-03-28T11:49:41Z | |
| dc.date.issued | 2001-08 | |
| dc.description.resumo | Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and 48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the acute phase led to PCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%. In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which 36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals had their antibodies titers decreased to such a level that the final results were considered as doubtful and 16 of them presented negative PCR. The molecular method represents a clear advantage over conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent chemotherapy. | pt_BR |
| dc.identifier.citation | BRITTO, Constança; SILVEIRA, Celeste; CARDOSO, Maria Angelica; MARQUES, Patricia; LUQUETTI, Alejandro; MACÊDO, Vanize; FERNANDES, Octavio. Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n.6, p. 823-826, Aug. 2001. | pt_BR |
| dc.identifier.doi | 10.1590/S0074-02762001000600014 | |
| dc.identifier.issn | 0074-0276 | |
| dc.identifier.issn | e- 1678-8060 | |
| dc.identifier.uri | http://repositorio.bc.ufg.br/handle/ri/14174 | |
| dc.language.iso | eng | pt_BR |
| dc.publisher.country | Brasil | pt_BR |
| dc.publisher.department | Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.subject | Trypanosoma cruzi | pt_BR |
| dc.subject | Kinetoplast DNA | pt_BR |
| dc.subject | Chagas disease | pt_BR |
| dc.subject | PCR | pt_BR |
| dc.subject | Treatment | pt_BR |
| dc.title | Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction | pt_BR |
| dc.type | Artigo | pt_BR |