Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction

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dc.creatorBritto, Constança
dc.creatorSilveira, Celeste
dc.creatorCardoso, Maria Angelica
dc.creatorMarques, Patricia
dc.creatorOstermayer, Alejandro Luquetti
dc.creatorMacêdo, Vanize
dc.creatorFernandes, Octavio
dc.date.accessioned2018-03-28T11:49:41Z
dc.date.available2018-03-28T11:49:41Z
dc.date.issued2001-08
dc.description.resumoPolymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and 48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the acute phase led to PCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%. In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which 36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals had their antibodies titers decreased to such a level that the final results were considered as doubtful and 16 of them presented negative PCR. The molecular method represents a clear advantage over conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent chemotherapy.pt_BR
dc.identifier.citationBRITTO, Constança; SILVEIRA, Celeste; CARDOSO, Maria Angelica; MARQUES, Patricia; LUQUETTI, Alejandro; MACÊDO, Vanize; FERNANDES, Octavio. Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n.6, p. 823-826, Aug. 2001.pt_BR
dc.identifier.doi10.1590/S0074-02762001000600014
dc.identifier.issn0074-0276
dc.identifier.issne- 1678-8060
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/14174
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectTrypanosoma cruzipt_BR
dc.subjectKinetoplast DNApt_BR
dc.subjectChagas diseasept_BR
dc.subjectPCRpt_BR
dc.subjectTreatmentpt_BR
dc.titleParasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reactionpt_BR
dc.typeArtigopt_BR

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