Antimicrobial activity evaluation of Hortia oreadica Groppo, Kallunki & Pirani essentials oils (leaves and flowers) and crude ethanol extract and fractions of leaves
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2015-06
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Resumo
Hortia oreadica (Rutaceae) known as “para-tudo, quina, quina do campo”
("for- everything, quinine, quinine of the field") is used in traditional medicine
locally to treat stomach pain and fevers. The aim of this study was evaluate the
antimicrobial activity of essential oils (leaves and flowers) and the crude ethanol
extract and fractions of leaves. The samples were collected in Pirenópolis, Goiás,
Brazil. The crude ethanol extract was obtained by maceration of the leaves
powdered. The essential oils were obtained by hydro distillation using a Clevenger
type apparatus and analyzed by GC/MS. The antimicrobial activity in vitro was
performed by broth micro dilution method. The crude ethanol extract showed good
antimicrobial activity against Candida krusei (MIC=31.25 μg/ml) and Candida
tropicalis (MIC=15.62 μg/ml) and moderate activity against Staphylococcus
epidermidis ATCC 12229 (MIC=250 μg/ml). The hexane fraction had moderate
activity against S. epidermidis ATCC 12229 (MIC=250 μg/ml), Pseudomonas
aeruginosa SPM 1 (MIC=250 μg/ml) and Salmonella spp ATCC 194430 (MIC=250
μg/ml), the oils of leaves and flowers showed weak antimicrobial activity or were
inactive (MIC>500 μg/ml). This work represents the first study of antimicrobial
activity of essential oils of leaves and flowers of H. oreadica.
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Antimicrobial activity, Rutaceae, Essential oils, Hortia
Citação
FIUZA, Tatiana S. et al. Antimicrobial activity evaluation of Hortia oreadica Groppo, Kallunki & Pirani essentials oils (leaves and flowers) and crude ethanol extract and fractions of leaves. Research & Reviews: journal of pharmacognosy and phytochemistry, Hyderabad, v. 3, n. 2, p. 66-70, June 2015.