Interaction with Pantoea agglomerans modulates growth and melanization of Sporothrix brasiliensis and Sporothrix schenckii

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dc.creatorPaes, Rodrigo Almeida
dc.creatorSantos, Fabio Brito dos
dc.creatorOliveira, Manoel Marques Evangelista
dc.creatorBailão, Alexandre Melo
dc.creatorBorges, Clayton Luiz
dc.creatorAraújo, Glauber Ribeiro de Sousa
dc.creatorFrases Carvajal, Susana
dc.creatorSoares, Célia Maria de Almeida
dc.creatorOliveira, Rosely Maria Zancopé
dc.date.accessioned2024-08-16T12:09:18Z
dc.date.available2024-08-16T12:09:18Z
dc.date.issued2019
dc.description.abstractSporothrix brasiliensis and Sporothrix schenckii stand as the most virulent agents of sporotrichosis, a worldwide-distributed subcutaneous mycosis. The origin of Sporothrix virulence seems to be associated with fungal interactions with organisms living in the same environment. To assess this hypothesis, the growth of these two species in association with Pantoea agglomerans, a bacterium with a habitat similar to Sporothrix spp., was evalu ated. Growth, melanization, and gene expression of the fungus were compared in the presence or absence of the bacterium in the same culture medium. Both S. brasiliensis and S. schenckii grew in contact with P. agglomerans yielding heavily melanized conidia after 5 days of incubation at 30 C in Sabouraud agar. This increased melanin production occurred around bacte rial colonies, suggesting that fungal melanization is triggered by a diffusible bacterial product, which is also supported by a similar pattern of melanin production during Sporothrix spp. growth in contact with heat-killed P. agglomerans. Growth of P. agglomerans was similar in the presence or absence of the fungus. However, the growth of S. brasiliensis and S. schenckii was initially inhibited, but further enhanced when these species were co-cultured with P. agglomerans. Moreover, fungi were able to use killed bacteria as both carbon and nitrogen sources for growth. Representational difference analysis identi- fied overexpressed genes related to membrane trans port when S. brasiliensis was co-cultured with the bacteria. The down-regulation of metabolism-related genes appears to be related to nutrient availability during bacterial exploitation. These findings can lead to a better knowledge on Sporothrix ecology and virulence.
dc.identifier.citationALMEIDA-PAES, Rodrigo et al. Interaction with Pantoea agglomerans modulates growth and melanization of Sporothrix brasiliensis and Sporothrix schenckii. Mycopathologia, Berlin, v. 184, n. 3, p. 367-381, 2019. DOI: 10.1007/s11046-019-00350-x. Disponível em: https://link.springer.com/article/10.1007/s11046-019-00350-x. Acesso em: 8 ago. 2024.
dc.identifier.doi10.1007/s11046-019-00350-x
dc.identifier.issn0301-486X
dc.identifier.issne- 1573-0832
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/25292
dc.language.isoeng
dc.publisher.countryAlemanha
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPantoea agglomerans
dc.subjectSporothrix brasiliensis
dc.subjectSporothrix schenckii
dc.subjectMicrobial interaction
dc.titleInteraction with Pantoea agglomerans modulates growth and melanization of Sporothrix brasiliensis and Sporothrix schenckii
dc.typeArtigo

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